Lact-C2-GFP可视化活细胞中磷脂酰丝氨酸（PS）生物传感器质粒BioVector®

BioVector® Lact-C2-GFP质粒是一种表达载体，用作可视化活细胞中磷脂酰丝氨酸（PS）的生物传感器。它表达一个融合蛋白，该融合蛋白由牛源性乳粘连蛋白（Lactadherin）的C2结构域（Lact-C2）连接到绿色荧光蛋白（GFP）组成。

• 磷脂酰丝氨酸（PS）检测： 该质粒的主要功能是定位并追踪PS。PS是一种重要的阴离子磷脂，在健康细胞中通常位于质膜的内（胞质）小叶。它暴露在外小叶通常是细胞凋亡（程序性细胞死亡）和其他生物过程的信号。

• GFP融合蛋白： 表达的GFP-Lact-C2融合蛋白以钙非依赖性方式高亲和力、特异性地结合PS。当在细胞中表达时，GFP标签允许研究人员使用荧光显微镜观察细胞内PS的位置。

• 定位： 该探针主要标记质膜，但也可以装饰内体等内部细胞器，反映了PS在这些不同细胞隔室中的存在。

• 特异性： C2结构域特异性地与PS相互作用，并对不同膜环境中的PS具有选择性，使其成为区分PS与其他磷脂的可靠工具。

主要特点

• 插入基因： 该质粒包含牛源性乳粘连蛋白（MFGE8基因）的C2结构域和绿色荧光蛋白（GFP）的编码序列。

• 应用： 它广泛应用于细胞生物学研究，特别是活细胞成像，用于研究膜动力学、细胞凋亡和蛋白质定位。

• 表达： 它可用于哺乳动物细胞中的瞬时表达，或在DH5alpha等大肠杆菌菌株中扩增。

• 标记物： 根据具体的载体骨架（例如，p416或pEGFP-C2），它通常赋予细菌抗生素抗性（如氨苄青霉素或卡那霉素）以供筛选，并可能具有用于真核细胞稳定表达的选择标记物（如G418抗性）。

• Phosphatidylserine (PS) Detection: The primary function of the plasmid is to localize and track PS, an important anionic phospholipid that is typically found on the inner (cytosolic) leaflet of the plasma membrane in healthy cells. Its exposure on the outer leaflet often serves as a signal for apoptosis (programmed cell death) and other biological processes.

• GFP Fusion Protein: The expressed GFP-Lact-C2 fusion protein binds with high affinity and specificity to PS in a calcium-independent manner. When expressed in cells, the GFP tag allows researchers to visualize the location of PS within the cell using fluorescence microscopy.

• Localization: The probe primarily labels the plasma membrane, but it can also decorate internal organelles like endosomes, reflecting the presence of PS in these various cellular compartments.

• Specificity: The C2 domain specifically interacts with PS and is selective for PS in different membrane environments, making it a reliable tool for distinguishing PS from other phospholipids.

Key Features

• Insert Gene: The plasmid contains the coding sequences for the C2 domain of bovine lactadherin (MFGE8 gene) and the Green Fluorescent Protein (GFP).

• Applications: It is widely used in cell biology research, particularly for live-cell imaging, to study membrane dynamics, apoptosis, and protein localization.

• Expression: It can be used for transient expression in mammalian cells or propagated in E. coli strains like DH5alpha.

• Markers: Depending on the specific vector backbone (e.g., p416 or pEGFP-C2), it typically confers antibiotic resistance (like ampicillin or kanamycin) for selection in bacteria and may have selection markers (like G418 resistance) for stable expression in eukaryotic cells.

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

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