2P24 Pseudomonas fluorescens BioVector®丁香假单胞菌植物促生根际细菌PGPR BioVector NTCC保藏中心

BioVector® NTCC® 2P24 Pseudomonas fluorescens BioVector®丁香假单胞菌植物促生根际细菌PGPR


BioVector® 2P24 菌株是一种被充分表征的植物促生根际细菌（PGPR），具体鉴定为丁香假单胞菌（Pseudomonas fluorescens）2P24。该菌株从中国的带菌小麦土壤中分离得到，广泛应用于科学研究，特别是在植物病害生防、细菌调控以及废水处理等领域。

主要特性与研究应用
生防制剂：P. fluorescens 2P24 是一种有效的生防制剂，能够对抗多种土壤传播的植物病原体，例如立枯丝核菌（Rhizoctonia solani）和尖孢镰刀菌（Fusarium oxysporum）。
次级代谢产物：其主要的抑病机制涉及产生对抗多种病原体有毒性的抗生素2,4-二乙酰基间苯三酚（2,4-DAPG）。
调控研究：该菌株被用于研究复杂的调控网络，包括控制 2,4-DAPG 产生、运动性和生物膜形成等的 Gac/Rsm 系统、群体感应（QS）以及 III 型和 VI 型分泌系统（T3SS 和 T6SS）。
废水处理：研究表明，P. fluorescens 2P24 在从合成废水和生活废水中去除无机氮化合物（如铵和硝酸盐）方面表现出高效率，表明其在环境修复中具有潜在应用。
菌株变异体研究
科学家们已经从野生型 P. fluorescens 2P24 中生成并研究了各种突变菌株，以了解特定的基因功能：
rpoZ 突变体 (2P24-ΔrpoZ)：该突变体显示 2,4-DAPG 的产量显著减少，并且丧失了对抗立枯丝核菌的拮抗作用，表明 rpoZ 基因是生防性状的重要调节因子。
hfq 突变体 (PM107)：hfq 基因的缺失导致 2,4-DAPG 产量下降、生物膜形成减少，并削弱了在小麦根际定殖的能力。
rsmA rsmE 双突变体：该突变体产生高水平的 2,4-DAPG，并且由于高浓度抗生素的毒性而生长速率降低。
SPI-1 T3SS 突变体：invF 或 hilA 等基因发生突变的菌株显示出对某些真菌抵抗性的改变、对葡萄糖的趋化反应改变以及生物膜形成的变化，揭示了该分泌系统的多样化功能。
NTCC® Pseudomonas fluorescens 2P24, a beneficial bacterium used as a biocontrol agent to suppress soil-borne plant diseases. Its main mechanism of action is the production of the antibiotic compound 2,4-diacetylphloroglucinol (2,4-DAPG). The production of 2,4-DAPG is tightly regulated by a complex network of genes and proteins, including the Gac/Rsm pathway, quorum sensing, and other regulatory proteins like GidA and TrmE.
Key features of Pseudomonas fluorescens 2P24
Biocontrol agent: It is used to control root diseases in plants.
Antibiotic production: Its primary biocontrol activity comes from producing the antibiotic 2,4-DAPG.
Complex gene regulation: The production of 2,4-DAPG is controlled by a sophisticated regulatory network involving various factors.
Quorum sensing: Strain 2P24 uses a quorum-sensing system to regulate its biocontrol activity.
Other abilities: It can also form biofilms, colonize wheat roots, and has been studied for its ability to remove ammonium and nitrate from water.
Regulation of 2,4-DAPG production
The production of this crucial antibiotic is managed through a fine-tuned regulatory system:
Gac/Rsm pathway: A two-component system (GacS-GacA) activates this pathway, which in turn regulates the production of small non-coding RNAs (sRNAs) that are involved in controlling 2,4-DAPG synthesis.
RsmA and RsmE proteins: These proteins act as repressors by binding to the messenger RNA (mRNA) of genes needed to produce 2,4-DAPG.
Small non-coding RNAs (sRNAs): These sRNAs can fine-tune the levels of RsmA and RsmE, allowing the bacteria to precisely control 2,4-DAPG production.
Other regulatory factors: Other proteins, such as GidA, TrmE, and GrxD, have also been found to play roles in positively or negatively regulating 2,4-DAPG production through various mechanisms, such as modifying tRNAs or interacting with other proteins in the pathway.

Classification: Pseudomonas fluorescens, a plant growth-promoting rhizobacterium (PGPR).
Source: Isolated from wheat take-all decline soil in Shandong Province, China.
Function: Biocontrol agent against various soil-borne pathogens like Rhizoctonia solani and Fusarium oxysporum.
Key Metabolite: Produces the antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) as a primary mechanism of action.
Regulatory Systems: Activity is regulated by complex systems, including the GacA/GacS two-component system and quorum sensing mechanisms.
Environmental Role: Exhibits high efficiency in removing inorganic nitrogen from wastewater.
Research Use: Widely used in studies concerning bacterial regulation, plant-microbe interactions, and environmental applications.

Supplier来源:BioVector NTCC Inc.
Email: biovector@163.com
Website网址: http://www.biovector.net