pSAK277 农杆菌介导转化植物瞬时表达载体BioVector® plant transient expression vector BioVector NTCC保藏中心

BioVector® NTCC® pSAK277 农杆菌介导转化植物瞬时表达载体BioVector® plant transient expression vector

BioVector® pSAK277 载体主要用作植物研究中农杆菌介导转化方法的瞬时表达载体，特别是用于水果（桃子、草莓、梨）和烟草叶片中目标基因的过表达。
主要特性和信息
特性 描述
载体类型 农杆菌二元载体；植物瞬时表达载体
宿主生物 主要用于农杆菌菌株 GV3101 进行植物侵染
启动子 插入基因的表达由 CaMV35S 启动子驱动
植物筛选标记 在一般搜索中没有明确定义，但典型的植物载体使用卡那霉素或潮霉素抗性等标记
大肠杆菌筛选标记 包含细菌复制起点（ori），可能还有用于在大肠杆菌中筛选的抗生素抗性基因
用途 植物基因的过表达和功能分析，特别是与花青素生物合成相关的基因
对照 空 pSAK277 载体通常在实验中用作阴性对照

研究中的应用
研究人员使用标准克隆技术（例如，同源重组、EcoRI 和 XhoI 限制性位点）将感兴趣的基因插入 pSAK277 载体中，然后将其转化到根癌农杆菌中。转化后的农杆菌随后用于侵染植物组织进行瞬时表达测定。
基因克隆：用于引入各种转录因子（如 MYB、bHLH、WRKY 家族基因）的全长编码序列（CDS）。
功能分析：通过观察产生的表型（例如，色素沉着或糖积累的变化）来证明基因功能。

有关详细的载体图谱或序列，请联系BioVector NTCC典型培养物保藏中心订购。

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net

The BioVector® pSAK277 vector is primarily used as a transient expression vector for the Agrobacterium-mediated transformation method in plant studies, particularly for the overexpression of genes of interest in fruits (peach, strawberry, pear) and tobacco leaves.
Key Features and Information
Feature  Description
Vector Type Agrobacterium binary vector; transient expression vector for plants
Host Organism Primarily used in Agrobacterium tumefaciens strain GV3101 for plant infiltration
Promoter Expression of the inserted gene is driven by the CaMV35S promoter
Selection Marker (Plants) Not explicitly defined in general searches, but typical plant vectors use markers like kanamycin or hygromycin resistance.
Selection Marker (E. coli) Contains a bacterial origin of replication (ori) and likely an antibiotic resistance gene for selection in E. coli.
Purpose Overexpression and functional analysis of plant genes, especially those related to anthocyanin biosynthesis.
Control The empty pSAK277 vector is frequently used as a negative control in experiments.
Phylogenetic analysis of cyclin-dependent kinase (CDK) genes ...
Phylogenetic analysis of cyclin-dependent kinase (CDK) genes ...
Phylogenetic analysis of cyclin-dependent kinase (CDK) genes ...
Usage in Research
Researchers insert their gene of interest into the pSAK277 vector using standard cloning techniques (e.g., homologous recombination, EcoRI and XhoI restriction sites) and then transform it into Agrobacterium tumefaciens. The transformed Agrobacterium is then used to infiltrate plant tissues for transient expression assays.
Gene Cloning: Used to introduce full-length coding sequences (CDS) of various transcription factors (like MYB, bHLH, WRKY family genes).
Functional Analysis: Demonstrates gene function by observing the resulting phenotype (e.g., changes in pigmentation or sugar accumulation).

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心
电话:400-800-2947
工作QQ/微信同号:1843439339
网址 www.biovector.net