H1-ASCL1-mCherry; GZLe014-A-2E细胞Cell Line BioVector NTCC®细胞保藏中心

[Designation名称] NTCC® Synonyms: H1-ASCL1-mCherry; GZLe014-A-2E Cell Line细胞株
BioVector NTCC Inc.

Synonyms细胞别名: Cell Line Name: WAe001-A-2E
Cross-references: hPSCreg; WAe001-A-2E
NTCC® Accession Number: CVCL_E7AS
Cross-references: BioSamples; SAMEA115345313
References identifiers: PubMed=39059080;
Comments: From: Guangzhou Laboratory; Guangzhou; China.
Comments: Characteristics: Using CRISPR/Cas9 a P2A-mCherry-LoxP-PGK-Puro-LoxP construct was inserted at the C-terminus of both alleles of ASCL1 (PubMed=39059080).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Comments: Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Comments: Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin: NCBI_TaxID=9606; ! Homo sapiens (Human)
Hierarchy: CVCL_9771 ! WA01
Sex of cell: Male
Age of donor at sampling: Blastocyst stage
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-NTCC® Accession Number: CVCL_E7AS
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法：
Medium培养基：NTCC® Synonyms: H1-ASCL1-mCherry; GZLe014-A-2E Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net