H9-CAG-ChR2-EYFP; H9-AAVS-Puro-CAG-ChR2-eYFP #11CB3; WISCe010-A-29细胞Cell Line BioVector NTCC®细胞保藏中心

[Designation名称] NTCC® Synonyms: H9-CAG-ChR2-EYFP; H9-AAVS-Puro-CAG-ChR2-eYFP #11CB3; WISCe010-A-29 Cell Line细胞株
BioVector NTCC Inc.

Synonyms细胞别名: Cell Line Name: WAe009-A-29
Cross-references: WiCell; h9-cag-chr2-eyfp
NTCC® Accession Number: CVCL_ZB43
Cross-references: hPSCreg; WAe009-A-29
Cross-references: Wikidata; Q98134755
References identifiers: PubMed=31981924;
Comments: From: University of Wisconsin; Madison; USA.
Comments: Registration: Swiss research registry; BAG-hES-IMP-0102.
Comments: Characteristics: Using CRISPR/Cas9 a PuroR-CAG-ChR2(H134R)-eYFP construct was introduced in the AAVS1 safe harbor locus (PubMed=31981924).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 8DNLG; YFP (Note=EYFP, yellow fluorescent protein derived from GFP).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; Q8RUT8; Chlamydomonas reinhardtii channelrhodopsin-2 (cop4) (Note=With p.His134Arg).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Comments: Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Comments: Cell type: Embryonic stem cell; CL=CL_0002322.
Species of origin: NCBI_TaxID=9606; ! Homo sapiens (Human)
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-NTCC® Accession Number: CVCL_ZB43
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法：
Medium培养基：NTCC® Synonyms: H9-CAG-ChR2-EYFP; H9-AAVS-Puro-CAG-ChR2-eYFP #11CB3; WISCe010-A-29 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net