NIH3T3 Halo-GLI2 and KIF7-Snap细胞Cell Line BioVector NTCC®细胞保藏中心
- 价 格：¥99850
- 货 号：NIH3T3 Halo-GLI2 and KIF7-Snap
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
[Designation名称] NTCC® Synonyms: NIH3T3 Halo-GLI2 and KIF7-Snap Cell Line细胞株
BioVector NTCC Inc.Synonyms细胞别名: Cell Line Name: NIH3T3 Halo-GLI2+KIF7-SnapComments: Characteristics: Transduced with a Kif7-SNAP tag construct (PubMed=40073114).NTCC® Accession Number: CVCL_E7UJReferences identifiers: PubMed=40073114;Comments: Characteristics: Transfected with both a pEF5-FRT-DEST-3XHA-Halo-mGLI2 and a pOG44 constructs (from parent cell line).Comments: Characteristics: Transfected with a pFRT/lacZeo construct that contains a FRT site for subsequent targeted integration of a Flp-In expression vector (from parent cell line).Comments: Genetic integration: Method=Transduction; Gene=HGNC; HGNC:7059; MGMT (Note=With p.Gly180_Asn207del and 19 mutations = SNAP-tag).Comments: Genetic integration: Method=Transduction; Gene=MGI; MGI:1098239; Kif7.Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).Comments: Genetic integration: Method=Transfection; Gene=MGI; MGI:95728; Gli2.Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (Note=Mutated = HaloTag).Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).Comments: Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-NTCC® Accession Number: CVCL_E7UJ
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法：
Medium培养基：NTCC® Synonyms: NIH3T3 Halo-GLI2 and KIF7-Snap Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net
BioVector NTCC Inc.Synonyms细胞别名: Cell Line Name: NIH3T3 Halo-GLI2+KIF7-SnapComments: Characteristics: Transduced with a Kif7-SNAP tag construct (PubMed=40073114).NTCC® Accession Number: CVCL_E7UJReferences identifiers: PubMed=40073114;Comments: Characteristics: Transfected with both a pEF5-FRT-DEST-3XHA-Halo-mGLI2 and a pOG44 constructs (from parent cell line).Comments: Characteristics: Transfected with a pFRT/lacZeo construct that contains a FRT site for subsequent targeted integration of a Flp-In expression vector (from parent cell line).Comments: Genetic integration: Method=Transduction; Gene=HGNC; HGNC:7059; MGMT (Note=With p.Gly180_Asn207del and 19 mutations = SNAP-tag).Comments: Genetic integration: Method=Transduction; Gene=MGI; MGI:1098239; Kif7.Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).Comments: Genetic integration: Method=Transfection; Gene=MGI; MGI:95728; Gli2.Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00722; Escherichia coli beta-galactosidase (lacZ).Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P0A3G2; Rhodococcus rhodochrous dhaA (Note=Mutated = HaloTag).Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P17493; Streptoalloteichus hindustanus ble (BleoR).Comments: Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-NTCC® Accession Number: CVCL_E7UJ
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法：
Medium培养基：NTCC® Synonyms: NIH3T3 Halo-GLI2 and KIF7-Snap Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net
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