RUES2-GLR细胞Cell Line BioVector NTCC®细胞保藏中心

[Designation名称] NTCC® Synonyms: RUES2-GLR Cell Line细胞株
BioVector NTCC Inc.

Synonyms细胞别名: Cell Line Name: RUESe002-A-6
Cross-references: hPSCreg; RUESe002-A-6
NTCC® Accession Number: CVCL_E9CV
Cross-references: BioSamples; SAMEA112765832
References identifiers: PubMed=29795348;
Comments: Registration: Swiss research registry; BAG-hES-IMP-0058.
Comments: Characteristics: Using CRISPR/Cas9 three independent fate reporters SOX2-mCitrine, SOX17-tdTomato and TBXT-mCerulean were produced. First a P2A-H2B-tdTomato construct was inserted at the C-terminus of SOX17, then a PA2-mCerulean was inserted at the C-terminus of TBXT (called BRA) and finally a mCitrine was fused to the C-terminus of SOX2 (PubMed=29795348).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; J2JWA; mCerulean (Note=Bright cyan fluorescent protein derived from GFP).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; 3Q37R; mCitrine (Note=Yellow fluorescent protein derived from GFP).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P33967; Bacillus cereus Bsr.
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Comments: Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P13249; Streptomyces alboniger pac (PuroR).
Comments: Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
Comments: Cell type: Embryonic stem cell; CL=CL_0002322.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-NTCC® Accession Number: CVCL_E9CV
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[Reference参考文献]
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培养方法：
Medium培养基：NTCC® Synonyms: RUES2-GLR Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net