S2R+ Pex19KO细胞Cell Line BioVector NTCC®细胞保藏中心

[Designation名称] NTCC® Synonyms: S2R+ Pex19KO Cell Line细胞株
BioVector NTCC Inc.

Synonyms细胞别名: Cell Line Name: S2R+-Pex19-KO-A1
Cross-references: FlyBase_Cell_line; FBtc0000306
NTCC® Accession Number: CVCL_A5UI
Cross-references: DGRC; 306
Cross-references: Wikidata; Q107116823
Comments: Group: Insect cell line.
Comments: Characteristics: Transfected with a pMT-FLAG-Cas9 construct where the expression of N-terminally FLAG-tagged Cas9 is driven by a copper-inducible metallothionein promoter (from parent cell line).
Comments: Knockout cell: Method=CRISPR/Cas9; FlyBase_Gene; FBgn0032407; Pex19.
Comments: Genetic integration: Method=Protein trap; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With a N-termial FLAG tag).
Comments: Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
Comments: Breed/subspecies: Oregon-R; FlyBase_Strain=FBsn0000276.
Species of origin: NCBI_TaxID=7227; ! Drosophila melanogaster (Fruit fly)
Hierarchy: CVCL_UD30 ! S2R+-MT::Cas9
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-NTCC® Accession Number: CVCL_A5UI
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Hierarchy]
培养方法：
Medium培养基：NTCC® Synonyms: S2R+ Pex19KO Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net