NPCs cells BioVector® 肝非实质细胞 (Liver Non-Parenchymal Cells, NPCs)

竭诚为您服务！

BioVector NTCC典型培养物保藏中心: 联系人：Dr.Xu, Biovector NTCC Inc.; 电话：400-800-2947 工作微信:1843439339 (QQ同号); 邮件：Biovector@163.com; 手机：18901268599; 地址：北京; 已注册

BioVector® 肝非实质细胞 (Liver Non-Parenchymal Cells, NPCs)

BioVector® 肝非实质细胞 (NPCs)

物种：人 (Homo sapiens) 或啮齿类 (大鼠/小鼠)
细胞组成：肝脏中除肝细胞外的复杂细胞群体，主要包括：
- 肝窦内皮细胞 (LSECs): 构成肝血窦壁。
- 库普弗细胞 (Kupffer Cells): 肝脏驻留巨噬细胞。
- 肝星状细胞 (HSCs): 储存维生素 A，参与肝纤维化。
- 肝内胆管上皮细胞 (Cholangiocytes): 构成胆管系统。
主要特征：
- 免疫与代谢调节： NPCs 是肝脏免疫监视、炎症反应和细胞外基质沉积的核心。
- 相互作用：在生理状态下维持肝细胞功能，在病理状态下介导肝损伤。
培养指南：
- 培养基：推荐使用专门配制的 NPC 维持培养基（通常含有高质量 FBS、胰岛素和特定细胞因子）。
- 基质支持：通常需要涂覆 I 型胶原蛋白 (Collagen I) 或纤连蛋白。
- 培养模式：推荐使用 3D 肝微组织 (Liver Microtissues) 或肝芯片系统，以维持细胞间的复杂信号传递。
主要应用：肝毒性评估、非酒精性脂肪肝 (NAFLD/NASH) 建模、肝纤维化研究及药物诱导性肝损伤 (DILI) 监测。

BioVector® Liver Non-Parenchymal Cells (NPCs)

Species: Human (Homo sapiens) or Rodent (Rat/Mouse)
Cell Composition: A heterogeneous population of cells in the liver excluding hepatocytes, primarily consisting of:
- Liver Sinusoidal Endothelial Cells (LSECs): Lining the hepatic sinusoids.
- Kupffer Cells: Resident hepatic macrophages.
- Hepatic Stellate Cells (HSCs): Vitamin A-storing cells, central to fibrosis.
- Cholangiocytes: Epithelial cells lining the bile ducts.
Key Characteristics:
- Immune and Metabolic Regulation: NPCs are essential for hepatic immunosurveillance, inflammatory response, and extracellular matrix deposition.
- Cell-Cell Crosstalk: They maintain hepatocyte functionality under physiological conditions and mediate liver injury during pathogenesis.
Cell Culture Data:
- Medium: Use specialized NPC Maintenance Media (typically supplemented with high-quality FBS, insulin, and specific cytokines).
- Substrate: Plates often require coating with Collagen Type I or Fibronectin.
- Culture Formats: Highly recommended for 3D Liver Microtissues or Organ-on-a-Chip systems to preserve physiological cell-cell interactions.
Applications: Predicting drug-induced liver injury (DILI), modeling Non-Alcoholic Steatohepatitis (NASH), and studying hepatic fibrosis and inflammation pathways.