OCI-LY8 BioVector®人弥漫大B细胞淋巴瘤细胞系 / Human Diffuse Large B-cell Lymphoma Cell Line BioVector® NTCC典型培养物保藏中心

BioVector® OCI-LY8 人弥漫大B细胞淋巴瘤细胞系 / Human Diffuse Large B-cell Lymphoma Cell Line 数据说明书

产品名称: BioVector® OCI-LY8 细胞
同义词: BioVector® OCI-Ly8; BioVector® OCILY8; BioVector® OCI-LY08; BioVector® LY8
中文名称: BioVector® OCI-LY8 人弥漫性大B细胞淋巴瘤细胞系
英文名称: BioVector® OCI-LY8 Human Diffuse Large B-cell Lymphoma Cell Line
产品类别: 血液肿瘤细胞系
物种来源: 人 (Homo sapiens)
组织来源: 外周血
疾病: 弥漫性大B细胞淋巴瘤（DLBCL）；免疫母细胞性B细胞淋巴瘤
患者信息: 未知
构建时间: 1990年左右建立
形态特性: 悬浮生长，呈圆形或椭圆形，以中心母细胞形态为主

生长特性: 悬浮生长

构建方法: 从弥漫性大B细胞淋巴瘤患者的外周血样本中建立
特性特征:

1. EB病毒状态: EBV阴性

2. 分子亚类: 根据基因表达谱分析，属于活化B细胞样（ABC）亚型

3. “双重打击”淋巴瘤: 被归类为“双重打击”淋巴瘤衍生的细胞系

4. 染色体易位: 携带染色体8p23.1易位，涉及MASL1癌基因；该易位产生MASL1外显子1与14q21区域隐性外显子的嵌合转录本

5. 基因突变: TP53基因突变

6. 免疫表型: CD20+， CD10+， CD3-；Bcl-6阴性，MUM-1阴性

7. FTO表达: FTO（脂肪量和肥胖相关蛋白）表达水平较高，与利妥昔单抗耐药相关

8. 研究应用: 弥漫性大B细胞淋巴瘤（DLBCL）的生物学特性研究、药物筛选（特别是利妥昔单抗耐药机制研究）、治疗策略开发的重要体外模型
   培养基: BioVector® OCI-LY8 细胞专用完全培养基（推荐基础成分：RPMI-1640培养基，添加10%胎牛血清和1%青霉素-链霉素） -3-4
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C -3-4
   传代方法: 维持细胞密度在2×10⁵ - 1×10⁶ cells/mL之间，每2-3天传代或更换新鲜培养基
   冻存液: BioVector® 细胞冻存液（推荐配方：90%胎牛血清 + 10% DMSO）
   质控载体: BioVector® pUC19 质粒
   宿主菌: BioVector® DH5α 化学感受态细胞
   质量控制: 支原体检测：阴性；无菌检测：阴性；STR分型：已验证

9. 

10. STR分型数据:
    Amelogenin: X
    CSF1PO: 10,12
    D13S317: 11
    D16S539: 11,12
    D5S818: 11,12
    D7S820: 9,10
    TH01: 8,9
    TPOX: 8,11
    vWA: 14,18
    储存与运输: 液氮或干冰运输与保存（-150°C至-190°C）
    生物安全等级: 1（需在二级生物安全柜内操作）
    仅供科研使用

English:
BioVector® OCI-LY8 Human Diffuse Large B-cell Lymphoma Cell Line Datasheet

Product Name: BioVector® OCI-LY8 Cells
Synonyms: BioVector® OCI-Ly8; BioVector® OCILY8; BioVector® OCI-LY08; BioVector® LY8
Chinese Name: BioVector® OCI-LY8 人弥漫性大B细胞淋巴瘤细胞系
English Name: BioVector® OCI-LY8 Human Diffuse Large B-cell Lymphoma Cell Line
Product Category: Hematologic Cancer Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Peripheral blood
Disease: Diffuse Large B-cell Lymphoma (DLBCL); Immunoblastic B-cell Lymphoma
Patient Information: Unknown
Year Established: Approximately 1990
Morphology: Suspension growth, round to oval cells, predominantly centroblastic morphology -2
Growth Characteristics: Suspension
Derivation: Established from peripheral blood sample of a patient with Diffuse Large B-cell Lymphoma
Characteristics:

1. EBV Status: Negative -5

2. Molecular Subtype: Classified as Activated B-cell-like (ABC) subtype based on gene expression profiling

3. "Double Hit" Lymphoma: Categorized as a cell line derived from "double hit" lymphoma -9

4. Chromosomal Translocation: Carries a translocation at chromosome 8p23.1 involving the MASL1 oncogene; this translocation produces a chimeric transcript of MASL1 exon 1 with a cryptic exon from the 14q21 genomic region -8

5. Gene Mutation: TP53 gene mutation -7

6. Immunophenotype: CD20+, CD10+, CD3-; Bcl-6 negative, MUM-1 negative -2

7. FTO Expression: High expression of FTO (Fat mass and obesity-associated protein), associated with Rituximab resistance -9

8. Research Applications: Important in vitro model for studying DLBCL biology, drug screening (particularly Rituximab resistance mechanisms), and therapeutic strategy development -9
   Culture Medium: BioVector® Complete Medium for OCI-LY8 Cells (Recommended composition: RPMI-1640 medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin) -3-4
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C -3-4
   Subculturing Method: Maintain cell density between 2×10⁵ - 1×10⁶ cells/mL; subculture or replenish with fresh medium every 2-3 days
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Control Vector: BioVector® pUC19 Plasmid
   Host Strain: BioVector® DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative; STR Profiling: Authenticated
   STR Profile Data:
   Amelogenin: X
   CSF1PO: 10,12
   D13S317: 11
   D16S539: 11,12
   D5S818: 11,12
   D7S820: 9,10
   TH01: 8,9
   TPOX: 8,11
   vWA: 14,18
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice (-150°C to -190°C)
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

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