K231 BioVector®人弥漫大B细胞淋巴瘤细胞系 / Human Diffuse Large B-cell Lymphoma Cell Line

BioVector® K231 人弥漫大B细胞淋巴瘤细胞系 / Human Diffuse Large B-cell Lymphoma Cell Line 数据说明书

产品名称: BioVector® K231 细胞
同义词: BioVector® KARPAS231; BioVector® KARPAS-231; BioVector® K-231
中文名称: BioVector® K231 人弥漫性大B细胞淋巴瘤细胞系
英文名称: BioVector® K231 Human Diffuse Large B-cell Lymphoma Cell Line
产品类别: 血液肿瘤细胞系
物种来源: 人 (Homo sapiens)
组织来源: 外周血
疾病: 弥漫性大B细胞淋巴瘤 (DLBCL)
患者信息: 71岁女性
形态特性: 悬浮生长，淋巴母细胞样
生长特性: 悬浮生长
构建方法: 从弥漫性大B细胞淋巴瘤患者的外周血样本中建立
特性特征:

1. 染色体易位: 携带 t(14;18)(q32;q21) 和 t(3;11)(q27;q23.1) 易位

2. EB病毒状态: 未报道，通常DLBCL细胞系多为EBV阴性

3. 基因表达特征: 与OCI-LY8等其他DLBCL细胞系相比，K231细胞系中miR-28表达水平较高；BCL-6和LPP蛋白表达水平较低

4. bcl-6基因状态: 由于未涉及Ig基因的易位，bcl-6 mRNA在该细胞系中未见表达

5. 研究应用: 弥漫性大B细胞淋巴瘤(DLBCL)的生物学特性研究、基因表达调控研究、药物筛选的重要体外模型
   培养基: BioVector® K231 细胞专用完全培养基（推荐基础成分：RPMI-1640培养基，添加10-20%胎牛血清和1%青霉素-链霉素）
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C
   传代方法: 维持细胞密度在2×10⁵ - 1×10⁶ cells/mL之间，每2-3天传代或更换新鲜培养基
   冻存液: BioVector® 细胞冻存液（推荐配方：90%胎牛血清 + 10% DMSO）
   质控载体: BioVector® pUC19 质粒
   宿主菌: BioVector® DH5α 化学感受态细胞
   质量控制: 支原体检测：阴性；无菌检测：阴性；STR分型：已验证（参考KARPAS231）
   储存与运输: 液氮或干冰运输与保存（-150°C至-190°C）
   生物安全等级: 1（需在二级生物安全柜内操作）
   仅供科研使用

English:
BioVector® K231 Human Diffuse Large B-cell Lymphoma Cell Line Datasheet

Product Name: BioVector® K231 Cells
Synonyms: BioVector® KARPAS231; BioVector® KARPAS-231; BioVector® K-231
Chinese Name: BioVector® K231 人弥漫性大B细胞淋巴瘤细胞系
English Name: BioVector® K231 Human Diffuse Large B-cell Lymphoma Cell Line
Product Category: Hematologic Cancer Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Peripheral blood
Disease: Diffuse Large B-cell Lymphoma (DLBCL)
Patient Information: 71-year-old female
Morphology: Suspension growth, lymphoblast-like
Growth Characteristics: Suspension
Derivation: Established from peripheral blood sample of a patient with Diffuse Large B-cell Lymphoma
Characteristics:

1. Chromosomal Translocations: Carries t(14;18)(q32;q21) and t(3;11)(q27;q23.1) translocations

2. EBV Status: Not reported; typically DLBCL cell lines are EBV negative

3. Gene Expression Profile: Compared to other DLBCL cell lines such as OCI-LY8, K231 cells show relatively high miR-28 expression levels and low BCL-6 and LPP protein expression levels

4. bcl-6 Gene Status: Due to the absence of Ig gene involvement in translocations, bcl-6 mRNA expression is not detected in this cell line

5. Research Applications: Important in vitro model for studying DLBCL biology, gene expression regulation, and drug screening
   Culture Medium: BioVector® Complete Medium for K231 Cells (Recommended composition: RPMI-1640 medium supplemented with 10-20% fetal bovine serum and 1% penicillin-streptomycin)
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C
   Subculturing Method: Maintain cell density between 2×10⁵ - 1×10⁶ cells/mL; subculture or replenish with fresh medium every 2-3 days
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Control Vector: BioVector® pUC19 Plasmid
   Host Strain: BioVector® DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative; STR Profiling: Authenticated (as KARPAS231)
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice (-150°C to -190°C)
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

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