MD901 BioVector®人弥漫大B细胞淋巴瘤细胞系 / Human Diffuse Large B-cell Lymphoma Cell Line BioVector NTCC典型培养物保藏中心

BioVector® MD901 人弥漫大B细胞淋巴瘤细胞系 / Human Diffuse Large B-cell Lymphoma Cell Line 数据说明书

产品名称: BioVector® MD901 细胞
同义词: BioVector® MD-901
中文名称: BioVector® MD901 人弥漫性大B细胞淋巴瘤细胞系
英文名称: BioVector® MD901 Human Diffuse Large B-cell Lymphoma Cell Line
产品类别: 血液肿瘤细胞系
物种来源: 人 (Homo sapiens)
组织来源: 不确定，可能为外周血或淋巴结
疾病: 弥漫性大B细胞淋巴瘤 (DLBCL)
患者信息: 未明确报道，基于细胞系建立时间推测为成人
形态特性: 悬浮生长，淋巴母细胞样
生长特性: 悬浮生长
构建方法: 从弥漫性大B细胞淋巴瘤患者样本中建立
分子特征:

1. 肿瘤亚型: 属于活化B细胞样（ABC）亚型

2. EB病毒状态: 未报道，通常DLBCL细胞系多为EBV阴性

3. CREBBP状态: CREBBP基因野生型，常用于CREBBP功能缺失研究

4. 基因表达特征: bcl-6 mRNA表达较强（涉及Ig基因易位）；miR-28有表达；LPP mRNA表达阳性

5. 蛋白表达: BCL-6蛋白表达水平较高；LPP蛋白表达阳性
   研究应用:

6. 淋巴瘤生物学: 弥漫性大B细胞淋巴瘤(DLBCL)的分子机制研究

7. 表观遗传学: CREBBP功能及其对增强子组蛋白乙酰化(H3K27ac)调控的研究

8. 药物筛选: 针对CREBBP突变淋巴瘤的HDAC3抑制剂等靶向治疗研究

9. 基因功能: SECISBP2等基因在淋巴瘤细胞增殖和凋亡中的作用研究
   培养基: BioVector® MD901 细胞专用完全培养基（推荐基础成分：RPMI-1640培养基或IMDM培养基，添加10%胎牛血清和1%青霉素-链霉素）
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C
   传代方法: 维持细胞密度在2×10⁵ - 1×10⁶ cells/mL之间，每2-3天传代或更换新鲜培养基
   冻存液: BioVector® 细胞冻存液（推荐配方：90%胎牛血清 + 10% DMSO）
   质控载体: BioVector® pUC19 质粒
   宿主菌: BioVector® DH5α 化学感受态细胞
   质量控制: 支原体检测：阴性；无菌检测：阴性；STR分型：信息缺失，建议使用者自行验证
   储存与运输: 液氮或干冰运输与保存（-150°C至-190°C）
   生物安全等级: 1（需在二级生物安全柜内操作）
   仅供科研使用

English:
BioVector® MD901 Human Diffuse Large B-cell Lymphoma Cell Line Datasheet

Product Name: BioVector® MD901 Cells
Synonyms: BioVector® MD-901
Chinese Name: BioVector® MD901 人弥漫性大B细胞淋巴瘤细胞系
English Name: BioVector® MD901 Human Diffuse Large B-cell Lymphoma Cell Line
Product Category: Hematologic Cancer Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Not specified, likely peripheral blood or lymph node
Disease: Diffuse Large B-cell Lymphoma (DLBCL)
Patient Information: Not reported, presumed adult based on cell line establishment period
Morphology: Suspension growth, lymphoblast-like
Growth Characteristics: Suspension
Derivation: Established from a patient sample with Diffuse Large B-cell Lymphoma
Molecular Characteristics:

1. Tumor Subtype: Activated B-cell-like (ABC) subtype

2. EBV Status: Not reported; typically DLBCL cell lines are EBV negative

3. CREBBP Status: CREBBP wild-type; commonly used for CREBBP loss-of-function studies

4. Gene Expression Profile: High bcl-6 mRNA expression (due to Ig gene translocation); miR-28 expression positive; LPP mRNA expression positive

5. Protein Expression: High BCL-6 protein expression level; LPP protein expression positive
   Research Applications:

6. Lymphoma Biology: Molecular mechanism studies of Diffuse Large B-cell Lymphoma (DLBCL)

7. Epigenetics: Studies on CREBBP function and its regulation of enhancer histone acetylation (H3K27ac)

8. Drug Screening: Targeted therapy research including HDAC3 inhibitors for CREBBP-mutant lymphomas

9. Gene Function: Studies on the role of genes such as SECISBP2 in lymphoma cell proliferation and apoptosis
   Culture Medium: BioVector® Complete Medium for MD901 Cells (Recommended composition: RPMI-1640 medium or IMDM medium supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin)
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C
   Subculturing Method: Maintain cell density between 2×10⁵ - 1×10⁶ cells/mL; subculture or replenish with fresh medium every 2-3 days
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 90% FBS + 10% DMSO)
   Control Vector: BioVector® pUC19 Plasmid
   Host Strain: BioVector® DH5α Chemically Competent Cells
   Quality Control: Mycoplasma: Negative; Sterility: Negative; STR Profiling: Information missing, recommended user authentication
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice (-150°C to -190°C)
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址

http://www.biovector.net