BioVector® EndoC-βH1 人胰腺β细胞系 / Human Pancreatic Beta Cell Line BioVector NTCC典型培养物保藏中心

BioVector® EndoC-βH1 人胰腺β细胞系 / Human Pancreatic Beta Cell Line 数据说明书

产品名称: BioVector® EndoC-βH1 细胞
同义词: BioVector® EndoC-β-H1; BioVector® EndoC‑βH1
中文名称: BioVector® EndoC-βH1 人胰腺β细胞系
英文名称: BioVector® EndoC-βH1 Human Pancreatic Beta Cell Line
产品类别: 胰腺细胞系；条件永生化细胞系
物种来源: 人 (Homo sapiens)
组织来源: 胰腺；胰岛
细胞类型: 胰腺β细胞
构建方法: 采用慢病毒转导技术，将人端粒酶逆转录酶和SV40大T抗原导入从孕早期(8-10周)胎儿胰腺组织分离的胰岛细胞，经克隆筛选和条件永生化技术建立
永生化机制: 携带loxP位点包围的永生化基因，可在Cre重组酶介导下切除，恢复细胞正常表型
形态特性: 上皮细胞样；聚集生长形成胰岛样细胞团
生长特性: 贴壁生长；依赖细胞外基质包被培养板
特性特征:

1. 胰岛素分泌: 高表达人胰岛素，葡萄糖刺激后胰岛素分泌增加2-3倍；EC50约6.8mM葡萄糖

2. β细胞标志物: 表达成熟β细胞标志物，包括PDX1、NKX6.1、MAFA、GLUT2、GCK等

3. 胰岛激素: 表达胰岛素，不表达胰高血糖素、生长抑素、胰多肽

4. 转录组特征: 转录组谱与人类原代胰岛β细胞高度相似

5. 分泌颗粒: 具有典型的β细胞分泌颗粒超微结构

6. 增殖特性: 需在含胰岛素、转铁蛋白、硒、β-巯基乙醇等因子的专用培养基中培养

7. 研究应用: 1型和2型糖尿病发病机制研究、β细胞功能研究、胰岛素分泌调控机制研究、药物筛选、毒性测试、β细胞替代治疗研究
   培养基: BioVector® EndoC-βH1 细胞专用完全培养基（推荐基础成分：DMEM低糖培养基，添加2% BSA、50 μM β-巯基乙醇、10 μg/mL 转铁蛋白、6.7 ng/mL 亚硒酸钠、100单位/mL 青霉素和100 μg/mL 链霉素）
   培养板包被: 培养板需预先用 BioVector® 细胞外基质包被液（含1.5 μg/cm² 纤维连接蛋白和0.2 μg/cm² 胶原蛋白IV）在37°C包被1小时
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C
   传代方法: 当细胞融合度达到80-90%时，推荐使用 BioVector® 专用传代试剂（如含EDTA的胰蛋白酶替代物）进行温和消化，按1:2至1:4比例进行传代；传代后需在包被板上培养；每3-4天更换新鲜培养基
   冻存液: BioVector® 细胞冻存液（推荐配方：70%完全培养基 + 20%胎牛血清 + 10% DMSO）
   质量控制: 支原体检测：阴性；无菌检测：阴性；胰岛素分泌功能验证：葡萄糖刺激响应阳性；β细胞标志物表达：阳性
   储存与运输: 液氮或干冰运输与保存
   生物安全等级: 1（需在二级生物安全柜内操作）
   仅供科研使用

English:
BioVector® EndoC-βH1 Human Pancreatic Beta Cell Line Datasheet

Product Name: BioVector® EndoC-βH1 Cells
Synonyms: BioVector® EndoC-β-H1; BioVector® EndoC‑βH1
Chinese Name: BioVector® EndoC-βH1 人胰腺β细胞系
English Name: BioVector® EndoC-βH1 Human Pancreatic Beta Cell Line
Product Category: Pancreatic Cell Line; Conditionally Immortalized Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Pancreas; Islets of Langerhans
Cell Type: Pancreatic Beta Cell
Derivation: Established from islet cells isolated from first trimester (8-10 weeks) fetal pancreatic tissue by lentiviral transduction with human telomerase reverse transcriptase and SV40 large T antigen, followed by clonal selection and conditional immortalization technology
Immortalization Mechanism: Carries loxP-flanked immortalizing genes that can be excised by Cre recombinase to restore normal cell phenotype
Morphology: Epithelial-like; grows in clusters forming islet-like cell aggregates
Growth Characteristics: Adherent; requires extracellular matrix-coated culture plates
Characteristics:

1. Insulin Secretion: High expression of human insulin; glucose-stimulated insulin secretion increased 2-3 fold; EC50 approximately 6.8 mM glucose

2. Beta Cell Markers: Expresses mature beta cell markers including PDX1, NKX6.1, MAFA, GLUT2, GCK, etc.

3. Islet Hormones: Expresses insulin; does not express glucagon, somatostatin, or pancreatic polypeptide

4. Transcriptome Profile: Transcriptome profile highly similar to human primary islet beta cells

5. Secretory Granules: Possesses typical ultrastructure of beta cell secretory granules

6. Proliferation Characteristics: Requires specialized medium containing insulin, transferrin, selenium, β-mercaptoethanol, and other factors

7. Research Applications: Type 1 and Type 2 diabetes pathogenesis studies, beta cell function research, insulin secretion regulation mechanism studies, drug screening, toxicity testing, beta cell replacement therapy research
   Culture Medium: BioVector® Complete Medium for EndoC-βH1 Cells (Recommended composition: DMEM low glucose medium supplemented with 2% BSA, 50 μM β-mercaptoethanol, 10 μg/mL transferrin, 6.7 ng/mL sodium selenite, 100 units/mL penicillin, and 100 μg/mL streptomycin)
   Plate Coating: Culture plates must be pre-coated with BioVector® Extracellular Matrix Coating Solution (containing 1.5 μg/cm² fibronectin and 0.2 μg/cm² collagen IV) at 37°C for 1 hour
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C
   Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector® Subculture Reagent (e.g., EDTA-containing trypsin substitute) for gentle dissociation. Subculture at a split ratio of 1:2 to 1:4. Culture on coated plates after subculture. Fresh medium renewal every 3-4 days
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 70% complete medium + 20% FBS + 10% DMSO)
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Insulin Secretion Function Validation: Glucose-stimulated response positive; Beta Cell Marker Expression: Positive
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

Chinese:
BioVector® EndoC-βH5 人胰腺β细胞系 / Human Pancreatic Beta Cell Line 数据说明书

产品名称: BioVector® EndoC-βH5 细胞
同义词: BioVector® EndoC-β-H5; BioVector® EndoC‑βH5
中文名称: BioVector® EndoC-βH5 人胰腺β细胞系
英文名称: BioVector® EndoC-βH5 Human Pancreatic Beta Cell Line
产品类别: 胰腺细胞系；条件永生化细胞系
物种来源: 人 (Homo sapiens)
组织来源: 胰腺；胰岛
细胞类型: 胰腺β细胞
构建方法: 从EndoC-βH1细胞系基础上进一步优化构建，通过基因修饰增强β细胞功能和成熟度
永生化机制: 携带loxP位点包围的永生化基因，可在Cre重组酶介导下切除，恢复细胞正常表型
形态特性: 上皮细胞样；聚集生长形成胰岛样细胞团
生长特性: 贴壁生长；依赖细胞外基质包被培养板
特性特征:

1. 胰岛素分泌: 高表达人胰岛素，葡萄糖刺激后胰岛素分泌显著增加；对葡萄糖刺激的敏感性高于EndoC-βH1细胞

2. β细胞标志物: 表达成熟β细胞标志物，包括PDX1、NKX6.1、MAFA、GLUT2、GCK等；表达水平高于EndoC-βH1

3. 成熟度: 细胞成熟度更高，更接近成人原代β细胞表型

4. 增殖特性: 需在含胰岛素、转铁蛋白、硒、β-巯基乙醇等因子的专用培养基中培养

5. 研究应用: 1型和2型糖尿病发病机制研究、β细胞功能研究、胰岛素分泌调控机制研究、药物筛选、毒性测试、β细胞成熟机制研究、β细胞替代治疗研究
   培养基: BioVector® EndoC-βH5 细胞专用完全培养基（推荐基础成分：DMEM低糖培养基，添加2% BSA、50 μM β-巯基乙醇、10 μg/mL 转铁蛋白、6.7 ng/mL 亚硒酸钠、100单位/mL 青霉素和100 μg/mL 链霉素）
   培养板包被: 培养板需预先用 BioVector® 细胞外基质包被液（含1.5 μg/cm² 纤维连接蛋白和0.2 μg/cm² 胶原蛋白IV）在37°C包被1小时
   培养条件: 气相：95%空气，5%二氧化碳；温度：37°C
   传代方法: 当细胞融合度达到80-90%时，推荐使用 BioVector® 专用传代试剂（如含EDTA的胰蛋白酶替代物）进行温和消化，按1:2至1:4比例进行传代；传代后需在包被板上培养；每3-4天更换新鲜培养基
   冻存液: BioVector® 细胞冻存液（推荐配方：70%完全培养基 + 20%胎牛血清 + 10% DMSO）
   质量控制: 支原体检测：阴性；无菌检测：阴性；胰岛素分泌功能验证：葡萄糖刺激响应阳性；β细胞标志物表达：阳性
   储存与运输: 液氮或干冰运输与保存
   生物安全等级: 1（需在二级生物安全柜内操作）
   仅供科研使用

English:
BioVector® EndoC-βH5 Human Pancreatic Beta Cell Line Datasheet

Product Name: BioVector® EndoC-βH5 Cells
Synonyms: BioVector® EndoC-β-H5; BioVector® EndoC‑βH5
Chinese Name: BioVector® EndoC-βH5 人胰腺β细胞系
English Name: BioVector® EndoC-βH5 Human Pancreatic Beta Cell Line
Product Category: Pancreatic Cell Line; Conditionally Immortalized Cell Line
Species of Origin: Human (Homo sapiens)
Tissue of Origin: Pancreas; Islets of Langerhans
Cell Type: Pancreatic Beta Cell
Derivation: Optimized and constructed based on the EndoC-βH1 cell line, with enhanced beta cell function and maturity through genetic modifications
Immortalization Mechanism: Carries loxP-flanked immortalizing genes that can be excised by Cre recombinase to restore normal cell phenotype
Morphology: Epithelial-like; grows in clusters forming islet-like cell aggregates
Growth Characteristics: Adherent; requires extracellular matrix-coated culture plates
Characteristics:

1. Insulin Secretion: High expression of human insulin; significantly increased glucose-stimulated insulin secretion; higher sensitivity to glucose stimulation compared to EndoC-βH1 cells

2. Beta Cell Markers: Expresses mature beta cell markers including PDX1, NKX6.1, MAFA, GLUT2, GCK, etc.; expression levels higher than EndoC-βH1

3. Maturity: Higher degree of cellular maturity, more closely resembling adult primary beta cell phenotype

4. Proliferation Characteristics: Requires specialized medium containing insulin, transferrin, selenium, β-mercaptoethanol, and other factors

5. Research Applications: Type 1 and Type 2 diabetes pathogenesis studies, beta cell function research, insulin secretion regulation mechanism studies, drug screening, toxicity testing, beta cell maturation mechanism studies, beta cell replacement therapy research
   Culture Medium: BioVector® Complete Medium for EndoC-βH5 Cells (Recommended composition: DMEM low glucose medium supplemented with 2% BSA, 50 μM β-mercaptoethanol, 10 μg/mL transferrin, 6.7 ng/mL sodium selenite, 100 units/mL penicillin, and 100 μg/mL streptomycin)
   Plate Coating: Culture plates must be pre-coated with BioVector® Extracellular Matrix Coating Solution (containing 1.5 μg/cm² fibronectin and 0.2 μg/cm² collagen IV) at 37°C for 1 hour
   Culture Conditions: Atmosphere: 95% air, 5% CO₂; Temperature: 37°C
   Subculturing Method: When cells reach 80-90% confluence, recommended to use BioVector® Subculture Reagent (e.g., EDTA-containing trypsin substitute) for gentle dissociation. Subculture at a split ratio of 1:2 to 1:4. Culture on coated plates after subculture. Fresh medium renewal every 3-4 days
   Freeze Medium: BioVector® Cell Freeze Medium (Recommended composition: 70% complete medium + 20% FBS + 10% DMSO)
   Quality Control: Mycoplasma: Negative; Sterility: Negative; Insulin Secretion Function Validation: Glucose-stimulated response positive; Beta Cell Marker Expression: Positive
   Storage and Shipment: Stored and shipped in liquid nitrogen or on dry ice
   Biosafety Level: 1 (Operate in a Class II biological safety cabinet)
   For Research Use Only

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

电话:400-800-2947

工作QQ/微信同号:1843439339

网址

http://www.biovector.net