YCUB-5 BioVector®人 B 细胞前体白血病细胞系/ Human B Cell Precursor Leukemia Cell Line

人 B 细胞前体白血病细胞系 YCUB-5 / Human B Cell Precursor Leukemia Cell Line YCUB-5

数据表 / Datasheet

细胞系信息

• 名称：BioVector® YCUB-5 细胞系

• 详细名称：人 B 细胞前体白血病细胞系 YCUB-5（携带 ETV6::RUNX1 融合基因）

• 来源：来源于一名 9 岁男孩 B 细胞前体急性淋巴细胞白血病（BCP-ALL）患者的外周血

• 细胞类型：B 细胞前体白血病（BCP-ALL）

• 形态：小而圆的细胞，悬浮生长，呈单细胞状态

• 免疫表型：CD10+、CD19+、CD20+、CD34+、CD38+、HLA-DR+、TdT+；CD3-、CD13-、CD33-、CD45RA+、CD58+

• 主要遗传特征：携带 ETV6::RUNX1（TEL::AML1）融合基因，为 t(12;21)(p13;q22) 易位的产物

• 其他特征：CD34 阳性提示该细胞系代表较早期的 B 细胞前体阶段；对化疗药物具有特定的敏感性谱

• 病毒检测：EBV-、HBV-、HCV-、HIV-1-、HIV-2-、HTLV-1/2-、MLV-

• 支原体检测：PCR 检测阴性

• STR 鉴定：通过 STR 分析验证，与参考数据库匹配

培养条件

• 培养基：BioVector® RPMI 1640 培养基，添加 10% BioVector® 胎牛血清

• 添加剂：建议添加 2 mM BioVector® L-谷氨酰胺

• 培养环境：37°C，5% CO₂，饱和湿度

• 传代方法：初始接种密度为 2–3 × 10⁵ 个细胞/mL；每 2–3 天按 1:2 至 1:4 比例传代；维持细胞密度在 3–8 × 10⁵ 个细胞/mL

• 冻存液：BioVector® 胎牛血清含 5–10% BioVector® DMSO

• 生物安全等级：BSL-1（建议按 BSL-2 操作）

特征与用途

• 携带 ETV6::RUNX1 融合基因，是儿童急性淋巴细胞白血病中最常见的染色体易位类型，广泛用于研究该融合基因在白血病发生、维持及预后中的作用机制

• 作为 ETV6::RUNX1 阳性细胞系模型，可用于研究该融合基因对细胞增殖、分化、凋亡及化疗敏感性的调控

• 在药物筛选研究中，用于评估新型靶向药物对该亚型白血病的疗效

• CD34 阳性、CD20 阳性表型使其适用于研究 B 细胞分化发育过程中 CD20 的表达调控及抗 CD20 靶向治疗的敏感性

Cell Line Information

• Name: BioVector® YCUB-5 Cell Line

• Detailed Name: Human B Cell Precursor Leukemia Cell Line YCUB-5 (carrying ETV6::RUNX1 fusion)

• Source: Derived from the peripheral blood of a 9-year-old boy with B-cell precursor acute lymphoblastic leukemia (BCP-ALL)

• Cell Type: B cell precursor leukemia (BCP-ALL)

• Morphology: Small, round cells growing singly in suspension

• Immunophenotype: CD10+, CD19+, CD20+, CD34+, CD38+, HLA-DR+, TdT+; CD3-, CD13-, CD33-, CD45RA+, CD58+

• Key Genetic Features: Carries the ETV6::RUNX1 (TEL::AML1) fusion gene resulting from t(12;21)(p13;q22) translocation

• Other Features: CD34 positivity indicates an earlier B-cell precursor stage; exhibits a specific sensitivity profile to chemotherapeutic agents

• Virus Testing: Negative for EBV, HBV, HCV, HIV-1, HIV-2, HTLV-1/2, MLV

• Mycoplasma: Negative by PCR assay

• Authentication: Verified by STR analysis matching the reference database

Culture Conditions

• Medium: BioVector® RPMI 1640 supplemented with 10% BioVector® fetal bovine serum

• Additives: 2 mM BioVector® L-glutamine recommended

• Culture Environment: 37°C, 5% CO₂, humidified atmosphere

• Subculturing: Seed at 2–3 × 10⁵ cells/mL initially; split at 1:2 to 1:4 ratio every 2–3 days; maintain cell density between 3–8 × 10⁵ cells/mL

• Cryopreservation: BioVector® fetal bovine serum with 5–10% BioVector® DMSO

• Biosafety Level: BSL-1 (standard BSL-2 practices recommended)

Characteristics & Applications

• Carries the ETV6::RUNX1 fusion gene, the most common chromosomal translocation in pediatric acute lymphoblastic leukemia; widely used to study the pathogenic mechanisms of this fusion in leukemogenesis, maintenance, and prognosis

• Serves as an ETV6::RUNX1-positive cell line model for investigating the regulatory roles of this fusion gene in cell proliferation, differentiation, apoptosis, and chemotherapy sensitivity

• Used in drug screening studies to evaluate the efficacy of novel targeted therapies against this leukemia subtype

• CD34-positive and CD20-positive immunophenotype makes it suitable for studying CD20 expression regulation during B-cell differentiation and sensitivity to CD20-targeted therapy

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