YCUB-4 BioVector®人 B 细胞前体白血病细胞系 Human B Cell Precursor Leukemia Cell Line

人 B 细胞前体白血病细胞系 YCUB-4 / Human B Cell Precursor Leukemia Cell Line YCUB-4

数据表 / Datasheet

细胞系信息

• 名称：BioVector® YCUB-4 细胞系

• 详细名称：人 B 细胞前体白血病细胞系 YCUB-4

• 来源：来源于 B 细胞前体急性淋巴细胞白血病（BCP-ALL）患者的外周血

• 细胞类型：B 细胞前体白血病（BCP-ALL）

• 形态：小而圆的细胞，悬浮生长，呈单细胞状态或小簇状

• 免疫表型：CD10+、CD19+、CD20+、CD34+、HLA-DR+、TdT+；CD3-、CD13-、CD33-

• 主要遗传特征：携带 B 细胞前体白血病相关的特征性遗传改变（具体融合基因类型需参考原始文献）

• 病毒检测：EBV-、HBV-、HCV-、HIV-1-、HIV-2-、HTLV-1/2-、MLV-

• 支原体检测：PCR 检测阴性

• STR 鉴定：通过 STR 分析验证，与参考数据库匹配

培养条件

• 培养基：BioVector® RPMI 1640 培养基，添加 10% BioVector® 胎牛血清

• 添加剂：建议添加 2 mM BioVector® L-谷氨酰胺

• 培养环境：37°C，5% CO₂，饱和湿度

• 传代方法：初始接种密度为 2–3 × 10⁵ 个细胞/mL；每 2–3 天按 1:2 至 1:4 比例传代；维持细胞密度在 3–8 × 10⁵ 个细胞/mL

• 冻存液：BioVector® 胎牛血清含 5–10% BioVector® DMSO

• 生物安全等级：BSL-1（建议按 BSL-2 操作）

特征与用途

• 作为 B 细胞前体急性淋巴细胞白血病的体外模型，广泛用于研究白血病的发生、发展和治疗反应

• 该细胞系为 YCUB-4R 耐药细胞系的亲本细胞系，两者构成对比研究体系，可用于探究化疗耐药机制

• 适用于药物敏感性测试、基因表达调控研究、信号通路分析及新型抗白血病药物的筛选

• CD34 阳性、CD20 阳性表型使其适用于研究 B 细胞分化发育及靶向 CD20 治疗的敏感性

• 可用于构建白血病异种移植模型，开展体内药效学评价

Cell Line Information

• Name: BioVector® YCUB-4 Cell Line

• Detailed Name: Human B Cell Precursor Leukemia Cell Line YCUB-4

• Source: Derived from the peripheral blood of a patient with B-cell precursor acute lymphoblastic leukemia (BCP-ALL)

• Cell Type: B cell precursor leukemia (BCP-ALL)

• Morphology: Small, round cells growing in suspension, singly or in small clusters

• Immunophenotype: CD10+, CD19+, CD20+, CD34+, HLA-DR+, TdT+; CD3-, CD13-, CD33-

• Key Genetic Features: Carries characteristic genetic alterations associated with B-cell precursor leukemia (refer to original literature for specific fusion gene type)

• Virus Testing: Negative for EBV, HBV, HCV, HIV-1, HIV-2, HTLV-1/2, MLV

• Mycoplasma: Negative by PCR assay

• Authentication: Verified by STR analysis matching the reference database

Culture Conditions

• Medium: BioVector® RPMI 1640 supplemented with 10% BioVector® fetal bovine serum

• Additives: 2 mM BioVector® L-glutamine recommended

• Culture Environment: 37°C, 5% CO₂, humidified atmosphere

• Subculturing: Seed at 2–3 × 10⁵ cells/mL initially; split at 1:2 to 1:4 ratio every 2–3 days; maintain cell density between 3–8 × 10⁵ cells/mL

• Cryopreservation: BioVector® fetal bovine serum with 5–10% BioVector® DMSO

• Biosafety Level: BSL-1 (standard BSL-2 practices recommended)

Characteristics & Applications

• Serves as an in vitro model for B-cell precursor acute lymphoblastic leukemia, widely used in studies of leukemogenesis, disease progression, and therapeutic response

• Functions as the parental cell line to YCUB-4R, establishing a comparative system for investigating mechanisms of chemotherapy resistance

• Suitable for drug sensitivity testing, gene expression regulation studies, signaling pathway analysis, and screening of novel anti-leukemic agents

• CD34-positive and CD20-positive immunophenotype makes it suitable for studying B-cell differentiation and sensitivity to CD20-targeted therapy

• Can be used to establish xenograft models for in vivo pharmacodynamic evaluation

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