BioVector® HASTR/ci35 人类星形胶质细胞永生化细胞系 / HASTR/ci35 Human Immortalized Astrocyte Cell Line

通用定义 / General Definition:

BioVector® HASTR/ci35 是一种源自人类胎儿脑组织的星形胶质细胞永生化细胞系。该细胞系通过转导**温度敏感型 SV40 大 T 抗原（tsA58）和人端粒酶逆转录酶（hTERT）**基因建立。其独特之处在于其“条件永生化”特性：在 33°C 下持续增殖，而在 37°-39°C 下则停止分裂并表现出更成熟的星形胶质细胞分化表型。它是研究神经炎症、血脑屏障（BBB）动力学及神经退行性疾病中星形胶质细胞功能的卓越工具。

BioVector® HASTR/ci35 is an immortalized human astrocyte cell line derived from fetal brain tissue. The line was established via transduction of the temperature-sensitive SV40 large T-antigen (tsA58) and human telomerase reverse transcriptase (hTERT). Its defining feature is "conditional immortalization": cells proliferate continuously at 33°C, while at 37°-39°C, they cease division and exhibit a more mature, differentiated astrocyte phenotype. It is a premier tool for studying neuroinflammation, blood-brain barrier (BBB) dynamics, and astrocyte function in neurodegenerative diseases.

BioVector® HASTR/ci35 细胞系技术说明书 (Cell Line Datasheet)

中文版说明书

1. 产品基本信息

• 产品名称： BioVector® HASTR/ci35 人星形胶质永生化细胞

• 细胞来源： 人类 (Human)

• 组织来源： 脑/星形胶质细胞 (Brain/Astrocyte)

• 生长特性： 贴壁生长

• 细胞形态： 典型的星形或梭形

2. 培养条件

• 基础培养基： BioVector® DMEM 培养基 或 BioVector® DMEM/F12 (1:1)

• 血清添加： 10% BioVector® 胎牛血清 (FBS)

• 培养环境： * 扩增阶段： 33°C，5% $CO_2$（激活大 T 抗原以促进增殖）

  • 实验阶段： 37°C - 39°C，5% $CO_2$（灭活大 T 抗原以诱导分化）

3. 细胞应用

• 神经毒性研究： 评估药物或化学物质对人类星形胶质细胞的影响。

• 血脑屏障模型： 与内皮细胞共培养构建体外 BBB 模型。

• 病毒学研究： 作为研究嗜神经病毒（如 HIV-1, Zika）感染的宿主细胞。

4. 注意事项

• 温度敏感性： 严格控制孵育温度。若需细胞增殖，切勿长时间放置在 37°C 以上环境中。

• 标记物表达： 细胞表达星形胶质细胞标志物，如胶质纤维酸性蛋白 (GFAP) 和 S100B。建议在分化状态下（37°C）检测。

English Datasheet

1. General Product Information

• Product Name: BioVector® HASTR/ci35 Human Immortalized Astrocyte Cell Line

• Organism: Human

• Tissue Source: Brain / Astrocyte

• Growth Properties: Adherent

• Morphology: Typically stellate or spindle-shaped

2. Culture Conditions

• Basal Medium: BioVector® DMEM Medium or BioVector® DMEM/F12 (1:1)

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS)

• Incubation: * Expansion: 33°C, 5% $CO_2$ (Active Large T-antigen for proliferation)

  • Experimental/Differentiation: 37°C - 39°C, 5% $CO_2$ (Inactive Large T-antigen for maturation)

3. Applications

• Neurotoxicity Studies: Assessing the impact of drugs or chemicals on human astrocytes.

• BBB Modeling: Co-culture with endothelial cells to establish in vitro Blood-Brain Barrier models.

• Virology: Serving as a host cell for studying neurotropic viral infections (e.g., HIV-1, Zika).

4. Key Usage Notes

• Temperature Sensitivity: Precise temperature control is mandatory. To maintain proliferation, avoid prolonged exposure to temperatures above 37°C.

• Marker Profile: The cells express astrocyte markers such as Glial Fibrillary Acidic Protein (GFAP) and S100B. Marker expression is optimally analyzed under differentiated conditions (37°C).

Note: Handle BioVector® HASTR/ci35 cells under Biosafety Level 2 (BSL-2) conditions. To ensure the unique temperature-sensitive phenotype remains stable, always use BioVector® DMEM Medium and BioVector® Fetal Bovine Serum.

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

E-mail: BioVector@163.com

http://www.biovector.net