BioVector® Pantoea ananatis PA13 欧文氏菌科泛菌属菌株 / BioVector® Pantoea ananatis PA13 Strain

通用定义 / General Definition:BioVector® Pantoea ananatis PA13 是一种多功能的革兰氏阴性、非芽孢杆菌。该菌株属于欧文氏菌科（Erwiniaceae），最初因其在多种农作物（如菠萝、洋葱、水稻）中作为致病菌或共生菌而被广泛研究。然而，PA13 菌株在合成生物学和代谢工程领域表现出极大的潜力，特别是其具有高效的生物质转化能力和对多种环境压力（如高渗透压和温差）的强耐受性。PA13 常被用作生产高附加值化学品（如类胡萝卜素、氨基酸）的底盘细胞，以及研究植物-微生物相互作用的遗传学模型。

BioVector® Pantoea ananatis PA13 is a versatile Gram-negative, non-spore-forming bacterium. Belonging to the family Erwiniaceae, this strain was initially studied for its role as either a pathogen or a symbiont in various crops (e.g., pineapple, onion, and rice). However, strain PA13 has shown immense potential in synthetic biology and metabolic engineering, particularly due to its efficient biomass conversion capabilities and robust tolerance to environmental stressors like high osmolarity and temperature fluctuations. PA13 is frequently utilized as a chassis for producing high-value chemicals (such as carotenoids and amino acids) and as a genetic model to study plant-microbe interactions.

BioVector® PA13 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® Pantoea ananatis PA13 菌株

• 生物分类： 泛菌属 (Pantoea)

• 基因组特征： 含有单圆染色体，部分变体携带质粒；具有完整的类胡萝卜素合成基因簇。

• 主要特性： 宽底物利用谱（可利用木糖、葡萄糖等）；极强的遗传操纵抗性稳定性。

2. 培养条件

• 培养基： BioVector® LB 培养基或 M9 最小培养基（用于代谢分析）。

• 培养温度： 最适生长温度为 30 - 37 摄氏度。

• 培养环境： 好氧培养，建议摇床转速为 200-220 rpm。

• 筛选抗性： 天然对部分抗生素具有抗性，实验室改造通常使用卡那霉素或氨苄青霉素。

3. 菌株应用

• 类胡萝卜素生产： 利用其内源性类胡萝卜素途径，通过代谢调控生产番茄红素、玉米黄质。

• 生物燃油研究： 作为底盘细胞转化农业废弃物（木质纤维素）为生物乙醇。

• 植物病理研究： 研究群体感应（Quorum Sensing）系统如何调节其在寄主植物中的定殖和致病性。

4. 注意事项

• 致病性级别： 虽然 PA13 主要用于科研，但鉴于其对某些植物的潜在致病性，应在生物安全一级（BSL-1）或二级（BSL-2，取决于当地规定）下操作。

• 遗传稳定性： 在大规模发酵过程中，建议定期通过 PCR 验证插入基因片的完整性。

English Datasheet

1. General Product Information

• Product Name: BioVector® Pantoea ananatis PA13 Strain

• Biological Classification: Genus Pantoea

• Genomic Features: Single circular chromosome; some variants carry plasmids; contains a complete endogenous carotenoid biosynthetic gene cluster.

• Key Characteristics: Broad substrate utilization profile (e.g., xylose, glucose); highly amenable to genetic manipulation with stable transgene maintenance.

2. Culture Conditions

• Media: BioVector® LB Medium or M9 Minimal Medium (for metabolic profiling).

• Incubation Temperature: Optimal growth at 30°C - 37°C.

• Culture Environment: Aerobic; recommended shaking speed at 200-220 rpm.

• Selection Markers: Exhibits natural resistance to certain antibiotics; laboratory modifications typically utilize Kanamycin or Ampicillin.

3. Applications

• Carotenoid Production: Leveraging endogenous pathways for the metabolic engineering of lycopene, zeaxanthin, and other pigments.

• Biofuel Research: Functioning as a chassis for converting lignocellulosic biomass (agricultural waste) into bioethanol or organic acids.

• Plant-Microbe Interactions: Investigating how Quorum Sensing systems regulate colonization and pathogenicity in host plant tissues.

4. Key Usage Notes

• Biosafety Level: While PA13 is a research-grade strain, due to its potential phytopathogenicity in specific crops, it should be handled under BSL-1 or BSL-2 conditions depending on regional agricultural regulations.

• Metabolic Flux: PA13 has a unique metabolic network that favors the non-mevalonate (MEP) pathway, which is critical for isoprenoid-derived product yields.

注意 / Note: BioVector® PA13 具有极强的环境适应性。在进行基因编辑实验（如 CRISPR/Cas9）时，建议针对 Pantoea 属特有的密码子偏好性进行载体优化。BioVector® PA13 exhibits exceptional environmental adaptability. For gene editing experiments (e.g., CRISPR/Cas9), BioVector® recommends optimizing vector sequences for the specific codon usage bias of the Pantoea genus.

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