BioVector® PEO1 人卵巢癌细胞系 / BioVector® PEO1 Human Ovarian Cancer Cell Line

通用定义 / General Definition:BioVector® PEO1 是一种源自人类卵巢浆液性腺癌 (Serous Ovarian Adenocarcinoma) 的细胞系。该细胞系取自一名对顺铂 (Cisplatin) 治疗表现出临床敏感性的患者的腹水。PEO1 最关键的生物学特征是携带 BRCA2 基因突变 (5193delA)，导致 BRCA2 蛋白功能缺失。这使得 PEO1 细胞对 DNA 损伤剂（如铂类药物）和 PARP 抑制剂 表现出极高的敏感性。它是研究同源重组修复 (HR) 缺陷、卵巢癌耐药演变（通常与其配对的耐药细胞系 PEO4 对比使用）以及合成致死疗法的核心模型。

BioVector® PEO1 is a human cell line derived from Serous Ovarian Adenocarcinoma. It was established from the ascitic fluid of a patient collected during a phase of clinical sensitivity to cisplatin-based chemotherapy. The most critical biological hallmark of PEO1 is the presence of a BRCA2 mutation (5193delA), resulting in the loss of functional BRCA2 protein. Consequently, PEO1 cells exhibit profound sensitivity to DNA-damaging agents (such as platinum compounds) and PARP inhibitors. It serves as a cornerstone model for studying Homologous Recombination (HR) deficiency, the evolution of drug resistance in ovarian cancer (often used in tandem with its resistant counterpart, PEO4), and synthetic lethality.

BioVector® PEO1 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® PEO1 人卵巢浆液性癌细胞

• 组织来源： 腹水 (Ascites)

• 分子特征：BRCA2 突变 (c.4965delA)；雌激素受体 (ER) 阳性；p53 突变。

• 生长特性： 贴壁生长。

• 细胞形态： 上皮样，通常呈鹅卵石状排列，倾向于形成紧密的细胞岛。

2. 培养条件

• 基础培养基： BioVector® RPMI 1640。

• 血清添加： 10% BioVector® 优质胎牛血清 (FBS)。

• 补充因子： 建议添加 2 mM L-谷氨酰胺 和 1 mM 丙酮酸钠；部分实验室建议添加 10 μg/mL 胰岛素以增强生长。

• 培养环境： 37 摄氏度，5% CO2。

• 传代比例： 1:2 至 1:4；该细胞系生长速度适中。

3. 细胞应用

• PARP 抑制剂筛选： 作为 HR 缺陷型阳性模型，测试 Olaparib、Niraparib 等药物的杀伤效力。

• 耐药机制研究： 与 PEO4（同一患者耐药后的细胞系，BRCA2 功能恢复）对比，研究回复突变引起的耐药。

• 内分泌研究： 由于其 ER 阳性特征，可用于研究激素对卵巢癌进展的影响。

4. 注意事项

• 基因稳定性： PEO1 具有发生回复突变（Reversion Mutation）的风险，从而获得铂类耐药。建议在低代数时使用，并定期验证其对顺铂的敏感性。

• 汇合度： 避免细胞过度生长，汇合度达到 80%-90% 时应及时传代，以防止细胞团块堆叠导致表型改变。

English Datasheet

1. General Product Information

• Product Name: BioVector® PEO1 Human Ovarian Serous Adenocarcinoma Cell Line

• Source: Ascites

• Molecular Profile:BRCA2 mutated (c.4965delA); Estrogen Receptor (ER) positive; p53 mutated.

• Growth Properties: Adherent.

• Morphology: Epithelial; cells display a cobblestone morphology and tend to grow in discrete islands.

2. Culture Conditions

• Basal Medium: BioVector® RPMI 1640.

• Serum Supplement: 10% BioVector® Fetal Bovine Serum (FBS).

• Supplements: 2 mM L-Glutamine and 1 mM Sodium Pyruvate are recommended; 10 μg/mL Insulin can be added to support optimal growth.

• Incubation: 37°C, 5% CO2.

• Subculturing: 1:2 to 1:4 ratio; exhibits a moderate doubling time.

3. Applications

• PARP Inhibitor Screening: Serving as a primary HR-deficient model to evaluate the efficacy of PARP inhibitors via synthetic lethality.

• Drug Resistance Research: Often paired with PEO4 (a resistant line from the same patient with a BRCA2 reversion mutation) to study molecular mechanisms of acquired chemoresistance.

• Hormonal Signaling: Utilizing the ER-positive status to investigate the role of estrogen in gynecological oncology.

4. Key Usage Notes

• Reversion Risk: PEO1 cells can spontaneously acquire reversion mutations in BRCA2 that restore DNA repair capacity. BioVector® recommends working with low-passage aliquots and periodically confirming platinum sensitivity.

• Morphology Management: Maintain the culture in the exponential growth phase. Allowing the cells to become over-confluent can lead to the formation of multi-layered aggregates which may alter experimental reproducibility.

注意 / Note: BioVector® PEO1 应在生物安全二级 (BSL-2) 条件下操作。在进行 DNA 修复相关研究时，建议使用无酚红培养基配合活性炭处理的血清，以消除内源性激素对 ER 阳性细胞的影响。BioVector® PEO1 should be handled under Biosafety Level 2 (BSL-2) conditions. For DNA repair and hormonal signaling assays, BioVector® suggests using phenol red-free media and charcoal-stripped FBS to minimize interference from exogenous estrogens.

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