BioVector® NK3.3 人源自然杀伤细胞系 / BioVector® NK3.3 Human Natural Killer Cell Line

通用定义 / General Definition:BioVector® NK3.3 是一种具有高度细胞毒性的人源非转化自然杀伤 (NK) 细胞系。该细胞系最初由 Jacki Kornbluth 实验室从一名健康志愿者的外周血中分离并建立。与常见的 NK 样肿瘤细胞系（如 NK-92）不同，NK3.3 被认为是非恶性转化的，且在生长过程中严格依赖白细胞介素-2 (IL-2)。NK3.3 保留了原代 NK 细胞的许多关键特征，包括对多种肿瘤靶细胞的自发杀伤活性，以及介导抗体依赖性细胞毒性 (ADCC) 的能力。它是研究 NK 细胞激活、抑制受体信号传导以及开发新型免疫疗法的极佳体外模型。

BioVector® NK3.3 is a highly cytotoxic, non-transformed human Natural Killer (NK) cell line. Originally isolated from the peripheral blood of a healthy donor by Jacki Kornbluth's laboratory, NK3.3 is distinct from common malignant NK cell lines (like NK-92) as it is considered non-transformed and exhibits a strict dependency on Interleukin-2 (IL-2) for growth. NK3.3 retains the key functional hallmarks of primary NK cells, including potent spontaneous cytotoxicity against various tumor targets and the ability to mediate Antibody-Dependent Cellular Cytotoxicity (ADCC). It serves as an excellent in vitro model for studying NK cell activation, inhibitory receptor signaling, and the development of novel immunotherapies.

BioVector® NK3.3 技术说明书 (Technical Datasheet)

中文版说明书 (Chinese Datasheet)

1. 产品基本信息

• 产品名称： BioVector® NK3.3 人自然杀伤细胞

• 组织来源： 人外周血 (Peripheral Blood)

• 细胞特性： 非转化、IL-2 依赖性、高度细胞毒性

• 表面标志物： CD56+, CD16+, CD3- (典型的 NK 细胞表型)

• 主要用途： 细胞毒性实验、ADCC 研究、NK 细胞受体生物学

2. 培养条件

• 基础培养基： BioVector® RPMI 1640。

• 血清添加： 10%-15% BioVector® 优质胎牛血清 (FBS)。

• 关键添加剂： 必须添加重组人 IL-2 (通常为 100-200 U/mL)。

• 补充因子： 2 mM L-谷氨酰胺、1% 非必需氨基酸、50 uM beta-巯基乙醇。

• 培养环境： 37 摄氏度，5% CO2，悬浮培养。


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3. 细胞应用

• 杀伤效率评价： 用于测试 NK 细胞对 K562（对 NK 敏感）等靶细胞的裂解能力。

• ADCC 实验： 评估治疗性抗体（如利妥昔单抗）结合靶细胞后诱导 NK3.3 杀伤的效果。

• 细胞因子产生： 刺激后产生 IFN-gamma 和 TNF-alpha 的能力。

4. 注意事项

• IL-2 敏感性： 缺乏 IL-2 会迅速导致细胞凋亡。建议每 2-3 天更换含新鲜 IL-2 的培养基。

• 细胞密度： 维持在 200,000 到 800,000 cells/mL 之间；密度过低会影响增殖，密度过高会导致活力下降。

English Datasheet

1. General Product Information

• Product Name: BioVector® NK3.3 Human Natural Killer Cell Line

• Source: Human peripheral blood

• Characteristics: Non-transformed, strictly IL-2 dependent, potent effector functions.

• Phenotype: CD56 positive, CD16 positive, CD3 negative.

• Primary Use: Assessing innate cytotoxicity, ADCC, and NK receptor signaling cascades.

2. Culture Conditions

• Basal Medium: BioVector® RPMI 1640.

• Serum Supplement: 10%-15% BioVector® Fetal Bovine Serum (FBS).

• Critical Factor: Supplemented with recombinant human IL-2 (100-200 U/mL).

• Supplements: 2 mM L-Glutamine, 1 mM Sodium Pyruvate, and 50 uM beta-mercaptoethanol.

• Incubation: 37°C, 5% CO2, suspension culture.


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3. Applications

• Cytotoxicity Assays: Standard effector for chromium release or flow-based killing assays against K562 or Raji cells.

• Immunotherapy Screening: Testing the efficacy of Bispecific Killer Cell Engagers (BiKEs) or TriKEs.

• Signal Transduction: Studying the phosphorylation of Syk, Zap70, and PLC-gamma upon activating receptor ligation.

4. Key Usage Notes

• Growth Kinetics: NK3.3 cells grow more slowly compared to malignant lines like NK-92. Patience is required during the initial expansion phase after thawing.

• Maintenance: Regularly monitor CD16 (Fc-gamma RIII) expression via flow cytometry if the cells are being used for ADCC assays, as expression levels can fluctuate.

注意 / Note: NK3.3 应在生物安全二级 (BSL-2) 条件下操作。该细胞系对操作过程中的物理压力较为敏感，复苏后建议通过缓慢添加培养基的方式进行过渡。NK3.3 should be handled under Biosafety Level 2 (BSL-2) conditions. This cell line is sensitive to physical stress; BioVector® recommends a gradual medium transition and gentle handling during the post-thaw recovery period.

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