BioVector® YIC-M153 细胞株（小鼠巨噬细胞） / BioVector® YIC-M153 Cell Line (Mouse Macrophage Cell Line)

一、 背景与来源说明 / Background & Origin

BioVector® YIC-M153（小鼠巨噬细胞株）是一种来源于小鼠（Mus musculus）组织/骨髓系的巨噬细胞样表达细胞模型（Mouse Macrophage Cell Line）。

巨噬细胞在宿主先天免疫（Innate Immunity）、炎性细胞因子级联反应、病原体吞噬消灭及抗原提呈过程中发挥核心作用。BioVector® YIC-M153 细胞株继承了典型小鼠巨噬细胞的免疫应答特性，能够高效响应脂多糖（LPS）、细胞因子（如 IFN-$\gamma$、TNF-$\alpha$）及多种病原相关分子模式（PAMPs/DAMPs）刺激。该细胞株常被用作小鼠免疫学研究、炎症信号通路探究、药物抗炎活性筛选及吞噬功能检测的细胞实验载体。

二、 细胞生物学特征 / Biological Characteristics

• 物种与来源 / Taxonomy & Source：小鼠（Mus musculus），免疫/造血系统（Macrophage Lineage）

• 生物安全等级 / Biosafety Level：BSL-1

• 生长特性 / Growth Properties：半贴壁/贴壁生长（Adherent / Semi-adherent），呈典型巨噬细胞样（Macrophage-like）多角形、伸出伪足（Pseudopodia）或圆形细胞形态

• 关键标志物 / Key Immunological Markers：

  • 巨噬细胞表面标记：F4/80(+)、CD11b(+)、CD68(+)

  • 功能特性：具备活跃的吞噬（Phagocytosis）能力与胞饮作用

• 功能特性 / Functional Attributes：

  • 炎症应答：经 LPS 或 IFN-$\gamma$ 刺激后可显著上调表达 iNOS、COX-2，并大量释放 TNF-$\alpha$、IL-6、IL-1$\beta$ 及 NO（一氧化氮）

  • 信号通路：广泛用于 NF-$\kappa$B、MAPK (p38, ERK, JNK) 及 NLRP3 炎性复合物信号通路分析

• 主要应用 / Key Applications：小鼠先天免疫与炎症机制研究、天然产物及新药抗炎活性筛选、吞噬与杀菌功能分析、免疫毒理学评估

三、 培养条件与传代指南 / Culture & Handling Protocols

1. 推荐培养基 / Complete Growth Medium

• 基础培养基：BioVector® High-Glucose DMEM 或 BioVector® RPMI-1640 Medium

• 添加成分：10% 灭活 BioVector® Fetal Bovine Serum (FBS) + 1% 双抗（Penicillin-Streptomycin）

• 培养环境：37 摄氏度，5% $\text{CO}_2$，高湿度培养箱

2. 复苏与传代操作 / Thawing & Subculture

1. 复苏：将冻存管快速置于 37 摄氏度水浴中摇晃融化（1–2 分钟）。加入 8–10 mL 预热的 BioVector® Complete Growth Medium，120 x g 离心 5 分钟，弃上清后重悬接种于培养瓶中。

2. 消化与吹打传代：

   • 细胞汇合度达到 70%–80% 时即可传代。

   • 特殊注意：巨噬细胞贴壁较紧密，建议使用 0.25% Trypsin-EDTA 孵育 2–4 分钟，或使用无酶细胞消化液/冷 PBS 浸泡后，用无菌细胞刮（Cell Scraper）或移液枪轻轻吹打脱落，避免剧烈机械力损伤细胞膜。

   • 按 1:2 至 1:4 比例传代。

3. 冻存保存 / Cryopreservation

• 冻存液配方：90% BioVector® FBS + 10% DMSO 或 70% BioVector® Basal Medium + 20% BioVector® FBS + 10% DMSO

• 保存条件：程序降温后长期保存于液氮（-196 摄氏度）。

四、 技术指标简表 / Technical Data Summary

BioVector® YIC-M153 细胞株（小鼠巨噬细胞） / BioVector® YIC-M153 Cell Line (Mouse Macrophage Cell Line)

1. Background and Origin

BioVector® YIC-M153 is a continuous mouse macrophage cell line derived from Mus musculus.

Macrophages play indispensable roles in innate immunity, inflammatory cascade activation, pathogen phagocytosis, and antigen presentation. The BioVector® YIC-M153 cell line retains characteristic macrophage biological responses, reacting robustly to lipopolysaccharide (LPS), pro-inflammatory cytokines (such as IFN-$\gamma$ and TNF-$\alpha$), and various pathogen-associated molecular patterns (PAMPs/DAMPs). It serves as a standard cellular platform for investigating murine immunology, inflammatory signal transduction, anti-inflammatory drug screening, and phagocytic activity.

2. Biological Characteristics

• Taxonomy & Source: Mus musculus, Hematopoietic/Macrophage Lineage

• Biosafety Level: BSL-1

• Growth Properties: Adherent / semi-adherent; displays macrophage-like polygonal, pseudopodia-extending, or rounded morphology

• Key Immunological Markers:

  • Macrophage Surface Markers: F4/80(+), CD11b(+), CD68(+)

  • Functional Activity: Active receptor-mediated phagocytosis and pinocytosis

• Functional Attributes:

  • Inflammatory Cascades: Responds to LPS and IFN-$\gamma$ by upregulating iNOS and COX-2, leading to significant release of NO, TNF-$\alpha$, IL-6, and IL-1$\beta$

  • Signaling Pathways: Widely utilized for dissecting NF-$\kappa$B, MAPK, and NLRP3 inflammasome pathways

• Primary Applications: Innate immune mechanism studies, anti-inflammatory drug discovery, phagocytosis and microbicidal assays, and immunotoxicity screening

3. Culture and Handling Protocols

Recommended Growth Medium

• Basal Medium: BioVector® High-Glucose DMEM or BioVector® RPMI-1640 Medium

• Supplements: 10% heat-inactivated BioVector® Fetal Bovine Serum (FBS) + 1% Penicillin-Streptomycin

• Culture Conditions: 37 degrees Celsius, 5% $\text{CO}_2$, humidified incubator

Thawing and Subculture Protocol

1. Thawing: Rapidly thaw in a 37 degrees Celsius water bath. Add 8–10 mL pre-warmed BioVector® Complete Growth Medium, centrifuge at 120 x g for 5 minutes, resuspend, and seed into culture flasks.

2. Passaging:

   • Subculture at 70–80% confluence.

   • Handling Note: Macrophages adhere firmly to plastic. Treat with 0.25% Trypsin-EDTA for 2–4 minutes at 37 degrees Celsius, or use non-enzymatic cell dissociation buffer / cold DPBS combined with a gentle cell scraper to harvest cells without damaging membrane receptors.

   • Split ratio: 1:2 to 1:4.

Cryopreservation

• Freezing Medium: 90% BioVector® FBS + 10% DMSO or 70% BioVector® Basal Medium + 20% BioVector® FBS + 10% DMSO

• Storage: Liquid nitrogen (-196 degrees Celsius).

4. Technical Data Summary

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