pKK233-2原核表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pKK233-2原核表达载体

Map图谱：

Sequence序列：

LOCUS       X70478                  4593 bp    DNA     linear   SYN 11-JUN-1993
DEFINITION  Plasmid pKK233-2 expression vector (from Pharmacia).
ACCESSION   X70478
VERSION     X70478.1
KEYWORDS    expression vector; pKK233-2.
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1
  AUTHORS   Kozak,M.
  TITLE     Comparison of initiation of protein synthesis in procaryotes,
            eucaryotes, and organelles
  JOURNAL   Microbiol. Rev. 47 (1), 1-45 (1983)
   PUBMED   6343825
REFERENCE   2
  AUTHORS   Amann,E. and Brosius,J.
  TITLE     'ATG vectors' for regulated high-level expression of cloned genes
            in Escherichia coli
  JOURNAL   Gene 40 (2-3), 183-190 (1985)
   PUBMED   3007288
REFERENCE   3
  AUTHORS   Straus,D. and Gilbert,W.
  TITLE     Chicken triosephosphate isomerase complements an Escherichia coli
            deficiency
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 82 (7), 2014-2018 (1985)
   PUBMED   3885220
REFERENCE   4
  AUTHORS   Shimizu,Y., Takabayashi,E., Yano,S.Y., Shimizu,N., Yamada,K. and
            Gushima,H.
  TITLE     Molecular cloning and expression in Escherichia coli of the cDNA
            coding for rat lipocortin I (calpactin II)
  JOURNAL   Gene 65 (1), 141-147 (1988)
   PUBMED   2969352
REFERENCE   5  (bases 1 to 4593)
  AUTHORS   Thomas,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAY-1993) Thomas C., Dept. Microbiology and
            Immunology, University of Adelaide, Australia:
            e-mail:cthomas@microb.adelaide.edu.au
COMMENT     The sequence data are compiled from direct sequencing data and data
            extracted from published sources. These were assembled by Pharmacia
            to form the final sequence.
            
            Taken directly from Pharmacia Catalog:
            Description: The pKK233-2 Expression Vector Kit is designed for
            direct cloning of eukaryotic genes in E. coli: The plasmid contains
            the highly expressed trc promoter (17 base pair spacing between the
            trp -35 region and the lac UV5-10 region), the lacZ ribosome
            binding site and ATG initiation codon (2). To prevent unstable
            replication, the strong rrnB transcription terminator has been
            introduced downstream of the Multiple Cloning Site. Digestion with
            Nco I exposes the start codon for direct ligation and expression of
            foreign proteins. Eukaryotic gene fragments lacking a prokaryotic
            ribosome binding site and/or an ATG can be inserted in the correct
            reading frame by using the three different Nco I linkers included
            in the kit. The Nco I recognition sequence, CCATGG, commonly occurs
            at the initiation codon of eukaryotic genes, allowing direct
            ligation to the vector (2,3). The pKK233-2 vedor has been
            successfully used to express truncated forms of the cI repressor
            protein from lambda (2). Herpes Simplex Type 1 glycoprotein D (2),
            rat lipocortin (4) and chicken triose phosphate isomerase (5).
FEATURES             Location/Qualifiers
     source          1..4593                     /organism="synthetic construct"                     /mol_type="other DNA"                     /db_xref="taxon:32630"ORIGIN      
        1 ccggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgccgttgc
       61 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
      121 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
      181 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
      241 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc
      301 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
      361 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
      421 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
      481 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
      541 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
      601 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
      661 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
      721 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac
      781 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
      841 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
      901 ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat
      961 caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg
     1021 tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg
     1081 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
     1141 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
     1201 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
     1261 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
     1321 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
     1381 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac
     1441 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc
     1501 attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa
     1561 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
     1621 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
     1681 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1741 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     1801 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     1861 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     1921 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     1981 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2041 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2101 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2161 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2221 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2281 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2341 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2401 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2461 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2521 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2581 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     2641 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2701 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2761 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2821 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2881 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2941 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3001 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3061 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3121 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3181 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3241 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3301 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3361 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3421 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3481 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     3541 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3601 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3661 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3721 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3781 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3841 atacatattt gaatgtattt agaaaaataa acaaaaagag tttgtagaaa cgcaaaaagg
     3901 ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct
     3961 gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc
     4021 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     4081 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg gagaccccac
     4141 actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac
     4201 cgcgctactg ccgccaggca aactgtttta tcagaccgct tctgcgttct gatttaatct
     4261 gtatcaggct gaaaatcttc tctcatccgc caaaacagcc aagcttggct gcagccatgg
     4321 tctgtttcct gtgtgaaatt gttatccgct cacaattcca cacattatac gagccggatg
     4381 attaattgtc aacagctcat ttcagaatat ttgccagaac cgtttatatg tcggcgcaaa
     4441 aaacattatc cagaacggga gtgcgccttg agcgacacga attatgcagt gatttacgac
     4501 ctgcacagcc aatccacagc ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt
     4561 gcagtcgatg ataagctgtc aaacatgaga att
//