| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pEBVHisA |
| 质粒类型: | 哺乳动物表达载体；附加体载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | RSV |
| 载体大小: | 10029 bp |
| 5' 测序引物及序列: | pREP Forward 5’ GCTCGATACAATAAACGCC 3’ |
| 3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
| 载体标签: | His tag (N-term) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Hygromycin |
| 克隆菌株: | DH5α等 |
| 宿主细胞（系）: | 人类细胞；灵长类细胞；犬类细胞 |
| 备注: | pEBVHis-lacZ载体是附加体型载体，可以在基因组外自主复制，不需要整合和转座到基因组上，无需包装成病毒，即可实现稳定表达。其它附加体载体有： pREP4 PCEP4 pREP7 pREP10 pEBNA-DEST pEBVHisA pEBVHisB pEBVHisC pREP4/CAT pEBVHis-lacZ |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
- BioVector NTCC典型培养物保藏中心
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- 已注册
pEBVHisA
pEBVHisA载体基本信息
pEBVHisA载体质粒图谱和多克隆位点信息
pEBVHisA载体简介
索取pEBVHis A,B,C载体说明书及蛋白表达与纯化的操作手册请E.Mail我们，或者在线留言。
索取pEBVHis A,B,C载体说明书及蛋白表达与纯化的操作手册请E.Mail我们，或者在线留言。
pEBVHisA载体序列
LOCUS Exported 10029 bp ds-DNA circular SYN 13-AUG-2016
KEYWORDS pEBVHisA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10029)
AUTHORS BioVector NTCC Inc.
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..10029
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 275..409
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
misc_feature 415..497
/note="MCS"
CDS complement(507..530)
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/note="Xpress(TM) tag"
/translation="DLYDDDDK"
CDS complement(534..566)
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS complement(570..587)
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
promoter complement(603..864)
/note="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
polyA_signal 1630..1678
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
CDS complement(1720..2757)
/codon_start=1
/product="hygromycin B phosphotransferase"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
MGEAN"
promoter complement(2800..2945)
/note="HSV TK promoter"
/note="herpes simplex virus thymidine kinase promoter"
rep_origin complement(3376..3964)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4135..4995)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4996..5100)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 tctagagtcg acctcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
61 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
121 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagccgga tcataatcag
181 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
241 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg atccggcctt
421 gccggcctcg atcgagcggc cgctagcaag cttcgaattc ccatatggta ccagctgcag
481 atctcgagct cggatcccca tcgatcctta tcgtcatcgt cgtacagatc ccgacccatt
541 tgctgtccac cagtcatgct agccatacca tgatgatgat gatgatgaga accccccatg
601 gaggtgcaca ccaatgtggt gaatggtcaa atggcgttta ttgtatcgag ctaggcactt
661 aaatacaata tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacccgtc
721 tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg
781 tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac
841 aagagtattg cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc
901 tgaggggact cctaaccgcg tacaaccgaa gccccgcttt tcgcctaaac acaccctagt
961 cccctcagat acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
1021 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
1081 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
1141 cggcatcaga gcagattgta ctgagagtgc accatacgga tctgcgatga taagctgtca
1201 aacatgagaa ttggtcgact agcttggcac gccagaaatc cgcgcggtgg tttttggggg
1261 tcgggggtgt ttggcagcca cagacgcccg gtgttcgtgt cgcgccagta catgcggtcc
1321 atgcccaggc catccaaaaa ccatgggtct gtctgctcag tccagtcgtg gacctgaccc
1381 cacgcaacgc ccaaaataat aacccccacg aaccataaac cattccccat gggggacccc
1441 gtccctaacc cacggggcca gtggctatgg cagggcctgc cgccccgacg ttggctgcga
1501 gccctgggcc ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt
1561 attggcccca atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc
1621 cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt tattgccgtc
1681 atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct cccccatctc
1741 ccgatccgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
1801 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
1861 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
1921 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
1981 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
2041 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
2101 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
2161 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
2221 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
2281 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
2341 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
2401 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
2461 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
2521 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
2581 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
2641 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
2701 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
2761 tcattgcccc ccgggatctg cggcacgctg ttgacgctgt taagcgggtc gctgcagggt
2821 cgctcggtgt tcgaggccac acgcgtcacc ttaatatgcg aagtggacct cggaccgcgc
2881 cgccccgact gcatctgcgt gttcgaattc gccaatgaca agacgctggg cggggtttgt
2941 gtcatcatag aactaaagac atgcaaatat atttcttccg gggacaccgc cagcaaacgc
3001 gagcaacggg ccacggggat gaagcagggc ggcacctcgc taacggattc accactccaa
3061 gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga
3121 acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg gggccgacgc
3181 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
3241 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
3301 agatgacgac catcagggac agcttcaagg ccagcaaaag gccaggaacc gtaaaaaggc
3361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
3421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
3481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
3541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
3601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
3661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
3721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
3781 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
3841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
3901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
3961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
4021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
4081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
4141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
4201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
4261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
4321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
4381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
4441 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
4501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
4561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
4621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
4681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
4741 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
4801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
4861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
4921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
4981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
5041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
5101 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
5161 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattctcat gtttgacagc
5221 ttatcatcgc gataagctga tcctcacagg ccgcacccag cttttcttcc gttgccccag
5281 tagcatctct gtctggtgac cttgaagagg aagaggaggg gtcccgagaa tccccatccc
5341 taccgtccag caaaaagggg gacgaggaat ttgaggcctg gcttgaggct caggacgcaa
5401 atcttgagga tgttcagcgg gagttttccg ggctgcgagt aattggtgat gaggacgagg
5461 atggttcgga ggatggggaa ttttcagacc tggatctgtc tgacagcgac catgaagggg
5521 atgagggtgg gggggctgtt ggagggggca ggagtctgca ctccctgtat tcactgagcg
5581 tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca
5641 ggtacaggac ctggaaatgg cctaggagag aagggagaca catctggacc agaaggctcc
5701 ggcggcagtg gacctcaaag aagagggggt gataaccatg gacgaggacg gggaagagga
5761 cgaggacgag gaggcggaag accaggagcc ccgggcggct caggatcagg gccaagacat
5821 agagatggtg tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa agggacccac
5881 ggtggaacag gagcaggagc aggagcggga ggggcaggag caggaggggc aggagcagga
5941 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagg ggcaggagca
6001 ggaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggagga
6061 ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca
6121 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggagca
6181 ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggaggg
6241 gcaggagggg caggaggggc aggagcagga ggggcaggag caggaggagg ggcaggaggg
6301 gcaggagggg caggagcagg aggggcagga gcaggagggg caggagcagg aggggcagga
6361 gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca
6421 ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg
6481 gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
6541 ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggggcagg agcaggaggt
6601 ggaggccggg gtcgaggagg cagtggaggc cggggtcgag gaggtagtgg aggccggggt
6661 cgaggaggta gtggaggccg ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt
6721 gaaagagcca gggggagagg tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt
6781 cagtcatcat catccgggtc tccaccgcgc aggccccctc caggtagaag gccatttttc
6841 caccctgtag gggaagccga ttattttgaa taccaccaag aaggtggccc agatggtgag
6901 cctgacgtgc ccccgggagc gatagagcag ggccccgcag atgacccagg agaaggccca
6961 agcactggac cccggggtca gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga
7021 aagcatcgtg gtcaaggagg ttccaacccg aaatttgaga acattgcaga aggtttaaga
7081 gctctcctgg ctaggagtca cgtagaaagg actaccgacg aaggaacttg ggtcgccggt
7141 gtgttcgtat atggaggtag taagacctcc ctttacaacc taaggcgagg aactgccctt
7201 gctattccac aatgtcgtct tacaccattg agtcgtctcc cctttggaat ggcccctgga
7261 cccggcccac aacctggccc gctaagggag tccattgtct gttatttcat ggtcttttta
7321 caaactcata tatttgctga ggttttgaag gatgcgatta aggaccttgt tatgacaaag
7381 cccgctccta cctgcaatat cagggtgact gtgtgcagct ttgacgatgg agtagatttg
7441 cctccctggt ttccacctat ggtggaaggg gctgccgcgg agggtgatga cggagatgac
7501 ggagatgaag gaggtgatgg agatgagggt gaggaagggc aggagtgatg taacttgtta
7561 ggagacgccc tcaatcgtat taaaagccgt gtattccccc gcactaaaga ataaatcccc
7621 agtagacatc atgcgtgctg ttggtgtatt tctggccatc tgtcttgtca ccattttcgt
7681 cctcccaaca tggggcaatt gggcataccc atgttgtcac gtcactcagc tccgcgctca
7741 acaccttctc gcgttggaaa acattagcga catttacctg gtgagcaatc agacatgcga
7801 cggctttagc ctggcctcct taaattcacc taagaatggg agcaaccagc aggaaaagga
7861 caagcagcga aaattcacgc ccccttggga ggtggcggca tatgcaaagg atagcactcc
7921 cactctacta ctgggtatca tatgctgact gtatatgcat gaggatagca tatgctaccc
7981 ggatacagat taggatagca tatactaccc agatatagat taggatagca tatgctaccc
8041 agatatagat taggatagcc tatgctaccc agatataaat taggatagca tatactaccc
8101 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc
8161 agatatagat taggatagca tatgctaccc agatatagat taggatagca tatgctatcc
8221 agatatttgg gtagtatatg ctacccagat ataaattagg atagcatata ctacccagat
8281 atagattagg atagcatatg ctacccagat atagattagg atagcctatg ctacccagat
8341 ataaattagg atagcatata ctacccagat atagattagg atagcatatg ctacccagat
8401 atagattagg atagcctatg ctacccagat atagattagg atagcatatg ctatccagat
8461 atttgggtag tatatgctac ccatggcaac attagcccac cgtgctctca gcgacctcgt
8521 gaatatgagg accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg taatttgtcc
8581 tccagatcgc agcaatcgcg cccctatctt ggcccgccca cctacttatg caggtattcc
8641 ccggggtgcc attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt agcggggtta
8701 caatcagcca agttattaca cccttatttt acagtccaaa accgcagggc ggcgtgtggg
8761 ggctgacgcg tgcccccact ccacaatttc aaaaaaaaga gtggccactt gtctttgttt
8821 atgggcccca ttggcgtgga gccccgttta attttcgggg gtgttagaga caaccagtgg
8881 agtccgctgc tgtcggcgtc cactctcttt ccccttgtta caaatagagt gtaacaacat
8941 ggttcacctg tcttggtccc tgcctgggac acatcttaat aaccccagta tcatattgca
9001 ctaggattat gtgttgccca tagccataaa ttcgtgtgag atggacatcc agtctttacg
9061 gcttgtcccc accccatgga tttctattgt taaagatatt cagaatgttt cattcctaca
9121 ctagtattta ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc acaatgccac
9181 cactgaaccc cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt gttttcgagc
9241 acctcacata caccttactg ttcacaactc agcagttatt ctattagcta aacgaaggag
9301 aatgaagaag caggcgaaga ttcaggagag ttcactgccc gctccttgat cttcagccac
9361 tgcccttgtg actaaaatgg ttcactaccc tcgtggaatc ctgaccccat gtaaataaaa
9421 ccgtgacagc tcatggggtg ggagatatcg ctgttcctta ggaccctttt actaacccta
9481 attcgatagc atatgcttcc cgttgggtaa catatgctat tgaattaggg ttagtctgga
9541 tagtatatac tactacccgg gaagcatatg ctacccgttt agggttaaca agggggcctt
9601 ataaacacta ttgctaatgc cctcttgagg gtccgcttat cggtagctac acaggcccct
9661 ctgattgacg ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg tacatgtccc
9721 ccagcattgg tgtaagagct tcagccaaga gttacacata aaggcaatgt tgtgttgcag
9781 tccacagact gcaaagtctg ctccaggatg aaagccactc agtgttggca aatgtgcaca
9841 tccatttata aggatgtcaa ctacagtcag agaacccctt tgtgtttggt ccccccccgt
9901 gtcacatgtg gaacagggcc cagttggcaa gttgtaccaa ccaactgaag ggattacatg
9961 cactgccccg cgaagaaggg gcagagatgt cgtagtcagg tttagttcgt ccggggcggg
10021 gatcgatcc
//
LOCUS Exported 10029 bp ds-DNA circular SYN 13-AUG-2016
KEYWORDS pEBVHisA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10029)
AUTHORS BioVector NTCC Inc.
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..10029
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 275..409
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
misc_feature 415..497
/note="MCS"
CDS complement(507..530)
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/note="Xpress(TM) tag"
/translation="DLYDDDDK"
CDS complement(534..566)
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS complement(570..587)
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
promoter complement(603..864)
/note="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
polyA_signal 1630..1678
/note="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
CDS complement(1720..2757)
/codon_start=1
/product="hygromycin B phosphotransferase"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
MGEAN"
promoter complement(2800..2945)
/note="HSV TK promoter"
/note="herpes simplex virus thymidine kinase promoter"
rep_origin complement(3376..3964)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4135..4995)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4996..5100)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 tctagagtcg acctcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
61 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
121 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagccgga tcataatcag
181 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
241 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg atccggcctt
421 gccggcctcg atcgagcggc cgctagcaag cttcgaattc ccatatggta ccagctgcag
481 atctcgagct cggatcccca tcgatcctta tcgtcatcgt cgtacagatc ccgacccatt
541 tgctgtccac cagtcatgct agccatacca tgatgatgat gatgatgaga accccccatg
601 gaggtgcaca ccaatgtggt gaatggtcaa atggcgttta ttgtatcgag ctaggcactt
661 aaatacaata tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacccgtc
721 tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg
781 tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac
841 aagagtattg cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc
901 tgaggggact cctaaccgcg tacaaccgaa gccccgcttt tcgcctaaac acaccctagt
961 cccctcagat acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
1021 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
1081 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
1141 cggcatcaga gcagattgta ctgagagtgc accatacgga tctgcgatga taagctgtca
1201 aacatgagaa ttggtcgact agcttggcac gccagaaatc cgcgcggtgg tttttggggg
1261 tcgggggtgt ttggcagcca cagacgcccg gtgttcgtgt cgcgccagta catgcggtcc
1321 atgcccaggc catccaaaaa ccatgggtct gtctgctcag tccagtcgtg gacctgaccc
1381 cacgcaacgc ccaaaataat aacccccacg aaccataaac cattccccat gggggacccc
1441 gtccctaacc cacggggcca gtggctatgg cagggcctgc cgccccgacg ttggctgcga
1501 gccctgggcc ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt
1561 attggcccca atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc
1621 cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt tattgccgtc
1681 atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct cccccatctc
1741 ccgatccgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
1801 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
1861 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
1921 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
1981 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
2041 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
2101 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
2161 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
2221 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
2281 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
2341 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
2401 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
2461 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
2521 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
2581 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
2641 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
2701 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
2761 tcattgcccc ccgggatctg cggcacgctg ttgacgctgt taagcgggtc gctgcagggt
2821 cgctcggtgt tcgaggccac acgcgtcacc ttaatatgcg aagtggacct cggaccgcgc
2881 cgccccgact gcatctgcgt gttcgaattc gccaatgaca agacgctggg cggggtttgt
2941 gtcatcatag aactaaagac atgcaaatat atttcttccg gggacaccgc cagcaaacgc
3001 gagcaacggg ccacggggat gaagcagggc ggcacctcgc taacggattc accactccaa
3061 gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga
3121 acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg gggccgacgc
3181 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
3241 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
3301 agatgacgac catcagggac agcttcaagg ccagcaaaag gccaggaacc gtaaaaaggc
3361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
3421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
3481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
3541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
3601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
3661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
3721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
3781 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
3841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
3901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
3961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
4021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
4081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
4141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
4201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
4261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
4321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
4381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
4441 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
4501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
4561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
4621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
4681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
4741 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
4801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
4861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
4921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
4981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
5041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
5101 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
5161 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattctcat gtttgacagc
5221 ttatcatcgc gataagctga tcctcacagg ccgcacccag cttttcttcc gttgccccag
5281 tagcatctct gtctggtgac cttgaagagg aagaggaggg gtcccgagaa tccccatccc
5341 taccgtccag caaaaagggg gacgaggaat ttgaggcctg gcttgaggct caggacgcaa
5401 atcttgagga tgttcagcgg gagttttccg ggctgcgagt aattggtgat gaggacgagg
5461 atggttcgga ggatggggaa ttttcagacc tggatctgtc tgacagcgac catgaagggg
5521 atgagggtgg gggggctgtt ggagggggca ggagtctgca ctccctgtat tcactgagcg
5581 tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca
5641 ggtacaggac ctggaaatgg cctaggagag aagggagaca catctggacc agaaggctcc
5701 ggcggcagtg gacctcaaag aagagggggt gataaccatg gacgaggacg gggaagagga
5761 cgaggacgag gaggcggaag accaggagcc ccgggcggct caggatcagg gccaagacat
5821 agagatggtg tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa agggacccac
5881 ggtggaacag gagcaggagc aggagcggga ggggcaggag caggaggggc aggagcagga
5941 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagg ggcaggagca
6001 ggaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggagga
6061 ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca
6121 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggagca
6181 ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggaggg
6241 gcaggagggg caggaggggc aggagcagga ggggcaggag caggaggagg ggcaggaggg
6301 gcaggagggg caggagcagg aggggcagga gcaggagggg caggagcagg aggggcagga
6361 gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca
6421 ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg
6481 gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
6541 ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggggcagg agcaggaggt
6601 ggaggccggg gtcgaggagg cagtggaggc cggggtcgag gaggtagtgg aggccggggt
6661 cgaggaggta gtggaggccg ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt
6721 gaaagagcca gggggagagg tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt
6781 cagtcatcat catccgggtc tccaccgcgc aggccccctc caggtagaag gccatttttc
6841 caccctgtag gggaagccga ttattttgaa taccaccaag aaggtggccc agatggtgag
6901 cctgacgtgc ccccgggagc gatagagcag ggccccgcag atgacccagg agaaggccca
6961 agcactggac cccggggtca gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga
7021 aagcatcgtg gtcaaggagg ttccaacccg aaatttgaga acattgcaga aggtttaaga
7081 gctctcctgg ctaggagtca cgtagaaagg actaccgacg aaggaacttg ggtcgccggt
7141 gtgttcgtat atggaggtag taagacctcc ctttacaacc taaggcgagg aactgccctt
7201 gctattccac aatgtcgtct tacaccattg agtcgtctcc cctttggaat ggcccctgga
7261 cccggcccac aacctggccc gctaagggag tccattgtct gttatttcat ggtcttttta
7321 caaactcata tatttgctga ggttttgaag gatgcgatta aggaccttgt tatgacaaag
7381 cccgctccta cctgcaatat cagggtgact gtgtgcagct ttgacgatgg agtagatttg
7441 cctccctggt ttccacctat ggtggaaggg gctgccgcgg agggtgatga cggagatgac
7501 ggagatgaag gaggtgatgg agatgagggt gaggaagggc aggagtgatg taacttgtta
7561 ggagacgccc tcaatcgtat taaaagccgt gtattccccc gcactaaaga ataaatcccc
7621 agtagacatc atgcgtgctg ttggtgtatt tctggccatc tgtcttgtca ccattttcgt
7681 cctcccaaca tggggcaatt gggcataccc atgttgtcac gtcactcagc tccgcgctca
7741 acaccttctc gcgttggaaa acattagcga catttacctg gtgagcaatc agacatgcga
7801 cggctttagc ctggcctcct taaattcacc taagaatggg agcaaccagc aggaaaagga
7861 caagcagcga aaattcacgc ccccttggga ggtggcggca tatgcaaagg atagcactcc
7921 cactctacta ctgggtatca tatgctgact gtatatgcat gaggatagca tatgctaccc
7981 ggatacagat taggatagca tatactaccc agatatagat taggatagca tatgctaccc
8041 agatatagat taggatagcc tatgctaccc agatataaat taggatagca tatactaccc
8101 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc
8161 agatatagat taggatagca tatgctaccc agatatagat taggatagca tatgctatcc
8221 agatatttgg gtagtatatg ctacccagat ataaattagg atagcatata ctacccagat
8281 atagattagg atagcatatg ctacccagat atagattagg atagcctatg ctacccagat
8341 ataaattagg atagcatata ctacccagat atagattagg atagcatatg ctacccagat
8401 atagattagg atagcctatg ctacccagat atagattagg atagcatatg ctatccagat
8461 atttgggtag tatatgctac ccatggcaac attagcccac cgtgctctca gcgacctcgt
8521 gaatatgagg accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg taatttgtcc
8581 tccagatcgc agcaatcgcg cccctatctt ggcccgccca cctacttatg caggtattcc
8641 ccggggtgcc attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt agcggggtta
8701 caatcagcca agttattaca cccttatttt acagtccaaa accgcagggc ggcgtgtggg
8761 ggctgacgcg tgcccccact ccacaatttc aaaaaaaaga gtggccactt gtctttgttt
8821 atgggcccca ttggcgtgga gccccgttta attttcgggg gtgttagaga caaccagtgg
8881 agtccgctgc tgtcggcgtc cactctcttt ccccttgtta caaatagagt gtaacaacat
8941 ggttcacctg tcttggtccc tgcctgggac acatcttaat aaccccagta tcatattgca
9001 ctaggattat gtgttgccca tagccataaa ttcgtgtgag atggacatcc agtctttacg
9061 gcttgtcccc accccatgga tttctattgt taaagatatt cagaatgttt cattcctaca
9121 ctagtattta ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc acaatgccac
9181 cactgaaccc cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt gttttcgagc
9241 acctcacata caccttactg ttcacaactc agcagttatt ctattagcta aacgaaggag
9301 aatgaagaag caggcgaaga ttcaggagag ttcactgccc gctccttgat cttcagccac
9361 tgcccttgtg actaaaatgg ttcactaccc tcgtggaatc ctgaccccat gtaaataaaa
9421 ccgtgacagc tcatggggtg ggagatatcg ctgttcctta ggaccctttt actaacccta
9481 attcgatagc atatgcttcc cgttgggtaa catatgctat tgaattaggg ttagtctgga
9541 tagtatatac tactacccgg gaagcatatg ctacccgttt agggttaaca agggggcctt
9601 ataaacacta ttgctaatgc cctcttgagg gtccgcttat cggtagctac acaggcccct
9661 ctgattgacg ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg tacatgtccc
9721 ccagcattgg tgtaagagct tcagccaaga gttacacata aaggcaatgt tgtgttgcag
9781 tccacagact gcaaagtctg ctccaggatg aaagccactc agtgttggca aatgtgcaca
9841 tccatttata aggatgtcaa ctacagtcag agaacccctt tgtgtttggt ccccccccgt
9901 gtcacatgtg gaacagggcc cagttggcaa gttgtaccaa ccaactgaag ggattacatg
9961 cactgccccg cgaagaaggg gcagagatgt cgtagtcagg tttagttcgt ccggggcggg
10021 gatcgatcc
//BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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