出品公司: | Invitrogen |
---|---|
载体名称: | pEBVHis-lacZ |
质粒类型: | 哺乳动物表达载体;附加体载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | RSV |
载体大小: | 13328 bp |
5' 测序引物及序列: | pREP Forward 5’ GCTCGATACAATAAACGCC 3’ |
3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
载体标签: | His tag (N-term) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Hygromycin |
克隆菌株: | DH5α等 |
宿主细胞(系): | 人类细胞;灵长类细胞;犬类细胞 |
备注: | pEBVHis-lacZ载体是附加体型载体,可以在基因组外自主复制,不需要整合和转座到基因组上,无需包装成病毒,即可实现稳定表达。其它附加体载体有: pREP4 PCEP4 pREP7 pREP10 pEBNA-DEST pEBVHisA pEBVHisB pEBVHisC pREP4/CAT pEBVHis-lacZ |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
- BioVector NTCC典型培养物保藏中心
- 联系人:Dr.Xu, Biovector NTCC Inc.
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- 已注册
pEBVHis-lacZ
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 13328 bp ds-DNA circular SYN 13-AUG-2016
DEFINITION synthetic circular DNA
KEYWORDS pEBVHis-lacz
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13328)
AUTHORS BioVector NTCC Inc.
FEATURES Location/Qualifiers
source 1..13328
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 276..410
/note="SV40 poly(A)"
/note="SV40 polyadenylation signal"
CDS complement(453..3500)
/codon_start=1
/gene="lacZ"
/product="beta-galactosidase"
/note="lacZ"
/translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
LNGEWRFAWFPVPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
SWSPSVSAEFQLSAGRYHYQLVWCQK"
CDS complement(3519..3542)
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/note="Xpress(TM) tag"
/translation="DLYDDDDK"
CDS complement(3546..3578)
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS complement(3582..3599)
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
promoter complement(3615..3877)
/note="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
polyA_signal 4647..4695
/note="HSV TK poly(A)"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
CDS complement(4760..5785)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
promoter complement(5825..5970)
/note="HSV TK promoter"
/note="herpes simplex virus thymidine kinase promoter"
rep_origin complement(6587..7175)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7346..8206)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8207..8311)
/gene="bla"
/note="AmpR promoter"
rep_origin 11065..12854
/note="oriP"
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
ORIGIN
1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
421 tgccggcctc gatcgagcgg ccgctagcaa gcttattttt gacaccagac caactggtaa
481 tggtagcgac cggcgctcag ctggaattcc gccgatactg acgggctcca ggagtcgtcg
541 ccaccaatcc ccatatggaa accgtcgata ttcagccatg tgccttcttc cgcgtgcagc
601 agatggcgat ggctggtttc catcagttgc tgttgactgt agcggctgat gttgaactgg
661 aagtcgccgc gccactggtg tgggccataa ttcaattcgc gcgtcccgca gcgcagaccg
721 ttttcgctcg ggaagacgta cggggtatac atgtctgaca atggcagatc ccagcggtca
781 aaacaggcgg cagtaaggcg gtcgggatag ttttcttgcg gccctaatcc gagccagttt
841 acccgctctg ctacctgcgc cagctggcag ttcaggccaa tccgcgccgg atgcggtgta
901 tcgctcgcca cttcaacatc aacggtaatc gccatttgac cactaccatc aatccggtag
961 gttttccggc tgataaataa ggttttcccc tgatgctgcc acgcgtgagc ggtcgtaatc
1021 agcaccgcat cagcaagtgt atctgccgtg cactgcaaca acgctgcttc ggcctggtaa
1081 tggcccgccg ccttccagcg ttcgacccag gcgttagggt caatgcgggt cgcttcactt
1141 acgccaatgt cgttatccag cggtgcacgg gtgaactgat cgcgcagcgg cgtcagcagt
1201 tgttttttat cgccaatcca catctgtgaa agaaagcctg actggcggtt aaattgccaa
1261 cgcttattac ccagctcgat gcaaaaatcc atttcgctgg tggtcagatg cgggatggcg
1321 tgggacgcgg cggggagcgt cacactgagg ttttccgcca gacgccactg ctgccaggcg
1381 ctgatgtgcc cggcttctga ccatgcggtc gcgttcggtt gcactacgcg tactgtgagc
1441 cagagttgcc cggcgctctc cggctgcggt agttcaggca gttcaatcaa ctgtttacct
1501 tgtggagcga catccagagg cacttcaccg cttgccagcg gcttaccatc cagcgccacc
1561 atccagtgca ggagctcgtt atcgctatga cggaacaggt attcgctggt cacttcgatg
1621 gtttgcccgg ataaacggaa ctggaaaaac tgctgctggt gttttgcttc cgtcagcgct
1681 ggatgcggcg tgcggtcggc aaagaccaga ccgttcatac agaactggcg atcgttcggc
1741 gtatcgccaa aatcaccgcc gtaagccgac cacgggttgc cgttttcatc atatttaatc
1801 agcgactgat ccacccagtc ccagacgaag ccgccctgta aacggggata ctgacgaaac
1861 gcctgccagt atttagcgaa accgccaaga ctgttaccca tcgcgtgggc gtattcgcaa
1921 aggatcagcg ggcgcgtctc tccaggtagc gaaagccatt ttttgatgga ccatttcggc
1981 acagccggga agggctggtc ttcatccacg cgcgcgtaca tcgggcaaat aatatcggtg
2041 gccgtggtgt cggctccgcc gccttcatac tgcaccgggc gggaaggatc gacagatttg
2101 atccagcgat acagcgcgtc gtgattagcg ccgtggcctg attcattccc cagcgaccag
2161 atgatcacac tcgggtgatt acgatcgcgc tgcaccattc gcgttacgcg ttcgctcatc
2221 gccggtagcc agcgcggatc atcggtcaga cgattcattg gcaccatgcc gtgggtttca
2281 atattggctt catccaccac atacaggccg tagcggtcgc acagcgtgta ccacagcgga
2341 tggttcggat aatgcgaaca gcgcacggcg ttaaagttgt tctgcttcat cagcaggata
2401 tcctgcacca tcgtctgctc atccatgacc tgaccatgca gaggatgatg ctcgtgacgg
2461 ttaacgcctc gaatcagcaa cggcttgccg ttcagcagca gcagaccatt ttcaatccgc
2521 acctcgcgga aaccgacatc gcaggcttct gcttcaatca gcgtgccgtc ggcggtgtgc
2581 agttcaacca ccgcacgata gagattcggg atttcggcgc tccacagttt cgggttttcg
2641 acgttcagac gtagtgtgac gcgatcggca taaccaccac gctcatcgat aatttcaccg
2701 ccgaaaggcg cggtgccgct ggcgacctgc gtttcaccct gccataaaga aactgttacc
2761 cgtaggtagt cacgcaactc gccgcacatc tgaacttcag cctccagtac agcgcggctg
2821 aaatcatcat taaagcgagt ggcaacatgg aaatcgctga tttgtgtagt cggtttatgc
2881 agcaacgaga cgtcacggaa aatgccgctc atccgccaca tatcctgatc ttccagataa
2941 ctgccgtcac tccaacgcag caccatcacc gcgaggcggt tttctccggc gcgtaaaaat
3001 gcgctcaggt caaattcaga cggcaaacga ctgtcctggc cgtaaccgac ccagcgcccg
3061 ttgcaccaca gatgaaacgc cgagttaacg ccatcaaaaa taattcgcgt ctggccttcc
3121 tgtagccagc tttcatcaac attaaatgtg agcgagtaac aacccgtcgg attctccgtg
3181 ggaacaaacg gcggattgac cgtaatggga taggttacgt tggtgtagat gggcgcatcg
3241 taaccgtgca tctgccagtt tgaggggacg acgacagtat cggcctcagg aagatcgcac
3301 tccagccagc tttccggcac cgcttctggt accggaaacc aggcaaagcg ccattcgcca
3361 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
3421 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
3481 tcacgacgtt gtaaaacgac gggatctatc attggatcct tatcgtcatc gtcgtacaga
3541 tcccgaccca tttgctgtcc accagtcatg ctagccatac catgatgatg atgatgatga
3601 gaacccccca tggaggtgca caccaatgtg gtgaatggtc aaatggcgtt tattgtatcg
3661 agctaggcac ttaaatacaa ttatctctgc aatgcggaat tcagtggttc gtccaatcca
3721 tgtcagacct gtctgttgcc ttcctaataa ggcacgatcg taccacctta cttccaccaa
3781 tcggcatgca cggtgctttt tctctccttg taaggcatgt tgctaactca tcgttaccat
3841 gttgcaagac tacaagtgta ttgcataaga ctacatttcc ccctccctat gcaaaagcga
3901 aactactata tcctgagggg actcctaacc gcgtacaacc gaagccccgc tgggcgccta
3961 aacacaccct agtcccctca gatacgcgta tatctggccc gtacatcgcg aagcagcgca
4021 aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta
4081 gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac
4141 tggcttaact atgcggcagc agagcagatt gtactgagag tgcaccatac aagctcagga
4201 tctgcgatga taagctgtca aacatgagaa ttggtcgacc actgggcgcc agaaatccgc
4261 gcggtggttt ttgggggtcg ggggtgtttg gcagccacag acgcccggtg ttcgtgtcgc
4321 gccagtacat gcggtccatg cccaggccat ccaaaaacca tgggtctgtc tgctcagtcc
4381 agtcgtggac cagaccccac gcaacgccca aaataataac ccccacgaac cataaaccat
4441 tccccatggg ggaccccgtc cctaacccac ggggccagtg gctatggcag ggcctgccgc
4501 cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa
4561 ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca
4621 gccctgggac cgaaccccgc gtttatgaac aaacgaccca acacccgtgc gttttattct
4681 gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag
4741 ttagcctccc ccatctcccc tattcctttg ccctcggacg agtgctgggg cgtcggtttc
4801 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
4861 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
4921 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
4981 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga
5041 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
5101 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
5161 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
5221 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
5281 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
5341 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
5401 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg
5461 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
5521 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg
5581 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
5641 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
5701 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
5761 tcgcggtgag ttcaggcttt ttcatatctc attgcccggg atctgcggca cgctgttgac
5821 gctgttaagc gggtcgctgc agggtcgctc ggtgttcgag gccacacgcg tcaccttaat
5881 atgcgaagtg gacctgggac cgcgccgccc cgactgcatc tgcgtgttcg aattcgccaa
5941 tgacaagacg ctgggcgggg tttgtgtcat catagaacta aagacatgca aatatatttc
6001 ttccggggac accgccagca aacgcgagca acgggccacg gggatgaagc agggcatggc
6061 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
6121 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
6181 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
6241 cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
6301 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt
6361 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct
6421 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg
6481 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg
6541 cgcagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
6601 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
6661 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
6721 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
6781 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
6841 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
6901 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
6961 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
7021 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
7081 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
7141 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
7201 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
7261 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
7321 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
7381 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
7441 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
7501 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
7561 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
7621 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
7681 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
7741 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
7801 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
7861 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
7921 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
7981 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
8041 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
8101 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
8161 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
8221 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
8281 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
8341 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
8401 ttcgtcttca agaattctca tgtttgacag cttatcatcg cgataagctg atcctcacag
8461 gccgcaccca gcttttcttc cgttgcccca gtagcatctc tgtctggtga ccttgaagag
8521 gaagaggagg ggtcccgaga atccccatcc ctaccgtcca gcaaaaaggg ggacgaggaa
8581 tttgaggcct ggcttgaggc tcaggacgca aatcttgagg atgttcagcg ggagttttcc
8641 gggctgcgag taattggtga tgaggacgag gatggttcgg aggatgggga attttcagac
8701 ctggatctgt ctgacagcga ccatgaaggg gatgagggtg ggggggctgt tggagggggc
8761 aggagtctgc actccctgta ttcactgagc gtcgtctaat aaagatgtct attgatctct
8821 tttagtgtga atcatgtctg acgaggggcc aggtacagga cctggaaatg gcctaggaga
8881 gaagggagac acatctggac cagaaggctc cggcggcagt ggacctcaaa gaagaggggg
8941 tgataaccat ggacgaggac ggggaagagg acgaggacga ggaggcggaa gaccaggagc
9001 cccgggcggc tcaggatcag ggccaagaca tagagatggt gtccggagac cccaaaaacg
9061 tccaagttgc attggctgca aagggaccca cggtggaaca ggagcaggag caggagcggg
9121 aggggcagga gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg
9181 aggggcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc
9241 aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg
9301 ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg
9361 agcaggagga ggggcaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc
9421 aggagcagga ggaggggcag gagcaggagg ggcaggaggg gcaggagggg caggagcagg
9481 aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag gaggggcagg
9541 agcaggaggg gcaggagcag gaggggcagg agcaggaggg gcaggagggg caggagcagg
9601 aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag gaggggcagg
9661 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggggcag gaggggcagg
9721 agcaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggagg
9781 ggcaggagca ggaggggcag gagcaggagg tggaggccgg ggtcgaggag gcagtggagg
9841 ccggggtcga ggaggtagtg gaggccgggg tcgaggaggt agtggaggcc gccggggtag
9901 aggacgtgaa agagccaggg ggggaagtcg tgaaagagcc agggggagag gtcgtggacg
9961 tggagaaaag aggcccagga gtcccagtag tcagtcatca tcatccgggt ctccaccgcg
10021 caggccccct ccaggtagaa ggccattttt ccaccctgta ggggaagccg attattttga
10081 ataccaccaa gaaggtggcc cagatggtga gcctgacgtg cccccgggag cgatagagca
10141 gggccccgca gatgacccag gagaaggccc aagcactgga ccccggggtc agggtgatgg
10201 aggcaggcgc aaaaaaggag ggtggtttgg aaagcatcgt ggtcaaggag gttccaaccc
10261 gaaatttgag aacattgcag aaggtttaag agctctcctg gctaggagtc acgtagaaag
10321 gactaccgac gaaggaactt gggtcgccgg tgtgttcgta tatggaggta gtaagacctc
10381 cctttacaac ctaaggcgag gaactgccct tgctattcca caatgtcgtc ttacaccatt
10441 gagtcgtctc ccctttggaa tggcccctgg acccggccca caacctggcc cgctaaggga
10501 gtccattgtc tgttatttca tggtcttttt acaaactcat atatttgctg aggttttgaa
10561 ggatgcgatt aaggaccttg ttatgacaaa gcccgctcct acctgcaata tcagggtgac
10621 tgtgtgcagc tttgacgatg gagtagattt gcctccctgg tttccaccta tggtggaagg
10681 ggctgccgcg gagggtgatg acggagatga cggagatgaa ggaggtgatg gagatgaggg
10741 tgaggaaggg caggagtgat gtaacttgtt aggagacgcc ctcaatcgta ttaaaagccg
10801 tgtattcccc cgcactaaag aataaatccc cagtagacat catgcgtgct gttggtgtat
10861 ttctggccat ctgtcttgtc accattttcg tcctcccaac atggggcaat tgggcatacc
10921 catgttgtca cgtcactcag ctccgcgctc aacaccttct cgcgttggaa aacattagcg
10981 acatttacct ggtgagcaat cagacatgcg acggctttag cctggcctcc ttaaattcac
11041 ctaagaatgg gagcaaccag catgcaggaa aaggacaagc agcgaaaatt cacgccccct
11101 tgggaggtgg cggcatatgc aaaggatagc actcccactc tactactggg tatcatatgc
11161 tgactgtata tgcatgagga tagcatatgc tacccggata cagattagga tagcatatac
11221 tacccagata tagattagga tagcatatgc tacccagata tagattagga tagcctatgc
11281 tacccagata taaattagga tagcatatac tacccagata tagattagga tagcatatgc
11341 tacccagata tagattagga tagcctatgc tacccagata tagattagga tagcatatgc
11401 tacccagata tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc
11461 cagatataaa ttaggatagc atatactacc ctaatctcta ttaggatagc atatgctacc
11521 cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc
11581 cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc
11641 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc
11701 cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccatg
11761 gcaacattag cccaccgtgc tctcagcgac ctcgtgaata tgaggaccaa caaccctgtg
11821 cttggcgctc aggcgcaagt gtgtgtaatt tgtcctccag atcgcagcaa tcgcgcccct
11881 atcttggccc gcccacctac ttatgcaggt attccccggg gtgccattag tggttttgtg
11941 ggcaagtggt ttgaccgcag tggttagcgg ggttacaatc agccaagtta ttacaccctt
12001 attttacagt ccaaaaccgc agggcggcgt gtgggggctg acgcgtgccc ccactccaca
12061 atttcaaaaa aaagagtggc cacttgtctt tgtttatggg ccccattggc gtggagcccc
12121 gtttaatttt cgggggtgtt agagacaacc agtggagtcc gctgctgtcg gcgtccactc
12181 tctttcccct tgttacaaat agagtgtaac aacatggttc acctgtcttg gtccctgcct
12241 gggacacatc ttaataaccc cagtatcata ttgcactagg attatgtgtt gcccatagcc
12301 ataaattcgt gtgagatgga catccagtct ttacggcttg tccccacccc atggatttct
12361 attgttaaag atattcagaa tgtttcattc ctacactagt atttattgcc caaggggttt
12421 gtgagggtta tattggtgtc atagcacaat gccaccactg aaccccccgt ccaaatttta
12481 ttctgggggc gtcacctgaa accttgtttt cgagcacctc acatacacct tactgttcac
12541 aactcagcag ttattctatt agctaaacga aggagaatga agaagcaggc gaagattcag
12601 gagagttcac tgcccgctcc ttgatcttca gccactgccc ttgtgactaa aatggttcac
12661 taccctcgtg gaatcctgac cccatgtaaa taaaaccgtg acagctcatg gggtgggaga
12721 tatcgctgtt ccttaggacc cttttactaa ccctaattcg atagcatatg cttcccgttg
12781 ggtaacatat gctattgaat tagggttagt ctggatagta tatactacta cccgggaagc
12841 atatgctacc cgtttagggt taacaagggg gccttataaa cactattgct aatgccctct
12901 tgagggtccg cttatcggta gctacacagg cccctctgat tgacgttggt gtagcctccc
12961 gtagtcttcc tgggcccctg ggaggtacat gtcccccagc attggtgtaa gagcttcagc
13021 caagagttac acataaaggc aatgttgtgt tgcagtccac agactgcaaa gtctgctcca
13081 ggatgaaagc cactcagtgt tggcaaatgt gcacatccat ttataaggat gtcaactaca
13141 gtcagagaac ccctttgtgt ttggtccccc cccgtgtcac atgtggaaca gggcccagtt
13201 ggcaagttgt accaaccaac tgaagggatt acatgcactg ccccgaatac aaaacaaaag
13261 cgctcctcgt accagcgaag aaggggcaga gatgccgtag tcaggtttag ttcgtccggc
13321 ggcggggc
//
LOCUS Exported 13328 bp ds-DNA circular SYN 13-AUG-2016 DEFINITION synthetic circular DNA KEYWORDS pEBVHis-lacz SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13328) AUTHORS BioVector NTCC Inc. FEATURES Location/Qualifiers source 1..13328 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 276..410 /note="SV40 poly(A)" /note="SV40 polyadenylation signal" CDS complement(453..3500) /codon_start=1 /gene="lacZ" /product="beta-galactosidase" /note="lacZ" /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS LNGEWRFAWFPVPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD SWSPSVSAEFQLSAGRYHYQLVWCQK" CDS complement(3519..3542) /codon_start=1 /product="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site" /note="Xpress(TM) tag" /translation="DLYDDDDK" CDS complement(3546..3578) /codon_start=1 /product="leader peptide from bacteriophage T7 gene 10" /note="T7 tag (gene 10 leader)" /note="promotes efficient translation in E. coli" /translation="MASMTGGQQMG" CDS complement(3582..3599) /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" promoter complement(3615..3877) /note="RSV promoter" /note="Rous sarcoma virus enhancer/promoter" polyA_signal 4647..4695 /note="HSV TK poly(A)" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" CDS complement(4760..5785) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /note="HygR" /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK E" promoter complement(5825..5970) /note="HSV TK promoter" /note="herpes simplex virus thymidine kinase promoter" rep_origin complement(6587..7175) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7346..8206) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(8207..8311) /gene="bla" /note="AmpR promoter" rep_origin 11065..12854 /note="oriP" /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" ORIGIN 1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct 61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc 181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga 241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct 421 tgccggcctc gatcgagcgg ccgctagcaa gcttattttt gacaccagac caactggtaa 481 tggtagcgac cggcgctcag ctggaattcc gccgatactg acgggctcca ggagtcgtcg 541 ccaccaatcc ccatatggaa accgtcgata ttcagccatg tgccttcttc cgcgtgcagc 601 agatggcgat ggctggtttc catcagttgc tgttgactgt agcggctgat gttgaactgg 661 aagtcgccgc gccactggtg tgggccataa ttcaattcgc gcgtcccgca gcgcagaccg 721 ttttcgctcg ggaagacgta cggggtatac atgtctgaca atggcagatc ccagcggtca 781 aaacaggcgg cagtaaggcg gtcgggatag ttttcttgcg gccctaatcc gagccagttt 841 acccgctctg ctacctgcgc cagctggcag ttcaggccaa tccgcgccgg atgcggtgta 901 tcgctcgcca cttcaacatc aacggtaatc gccatttgac cactaccatc aatccggtag 961 gttttccggc tgataaataa ggttttcccc tgatgctgcc acgcgtgagc ggtcgtaatc 1021 agcaccgcat cagcaagtgt atctgccgtg cactgcaaca acgctgcttc ggcctggtaa 1081 tggcccgccg ccttccagcg ttcgacccag gcgttagggt caatgcgggt cgcttcactt 1141 acgccaatgt cgttatccag cggtgcacgg gtgaactgat cgcgcagcgg cgtcagcagt 1201 tgttttttat cgccaatcca catctgtgaa agaaagcctg actggcggtt aaattgccaa 1261 cgcttattac ccagctcgat gcaaaaatcc atttcgctgg tggtcagatg cgggatggcg 1321 tgggacgcgg cggggagcgt cacactgagg ttttccgcca gacgccactg ctgccaggcg 1381 ctgatgtgcc cggcttctga ccatgcggtc gcgttcggtt gcactacgcg tactgtgagc 1441 cagagttgcc cggcgctctc cggctgcggt agttcaggca gttcaatcaa ctgtttacct 1501 tgtggagcga catccagagg cacttcaccg cttgccagcg gcttaccatc cagcgccacc 1561 atccagtgca ggagctcgtt atcgctatga cggaacaggt attcgctggt cacttcgatg 1621 gtttgcccgg ataaacggaa ctggaaaaac tgctgctggt gttttgcttc cgtcagcgct 1681 ggatgcggcg tgcggtcggc aaagaccaga ccgttcatac agaactggcg atcgttcggc 1741 gtatcgccaa aatcaccgcc gtaagccgac cacgggttgc cgttttcatc atatttaatc 1801 agcgactgat ccacccagtc ccagacgaag ccgccctgta aacggggata ctgacgaaac 1861 gcctgccagt atttagcgaa accgccaaga ctgttaccca tcgcgtgggc gtattcgcaa 1921 aggatcagcg ggcgcgtctc tccaggtagc gaaagccatt ttttgatgga ccatttcggc 1981 acagccggga agggctggtc ttcatccacg cgcgcgtaca tcgggcaaat aatatcggtg 2041 gccgtggtgt cggctccgcc gccttcatac tgcaccgggc gggaaggatc gacagatttg 2101 atccagcgat acagcgcgtc gtgattagcg ccgtggcctg attcattccc cagcgaccag 2161 atgatcacac tcgggtgatt acgatcgcgc tgcaccattc gcgttacgcg ttcgctcatc 2221 gccggtagcc agcgcggatc atcggtcaga cgattcattg gcaccatgcc gtgggtttca 2281 atattggctt catccaccac atacaggccg tagcggtcgc acagcgtgta ccacagcgga 2341 tggttcggat aatgcgaaca gcgcacggcg ttaaagttgt tctgcttcat cagcaggata 2401 tcctgcacca tcgtctgctc atccatgacc tgaccatgca gaggatgatg ctcgtgacgg 2461 ttaacgcctc gaatcagcaa cggcttgccg ttcagcagca gcagaccatt ttcaatccgc 2521 acctcgcgga aaccgacatc gcaggcttct gcttcaatca gcgtgccgtc ggcggtgtgc 2581 agttcaacca ccgcacgata gagattcggg atttcggcgc tccacagttt cgggttttcg 2641 acgttcagac gtagtgtgac gcgatcggca taaccaccac gctcatcgat aatttcaccg 2701 ccgaaaggcg cggtgccgct ggcgacctgc gtttcaccct gccataaaga aactgttacc 2761 cgtaggtagt cacgcaactc gccgcacatc tgaacttcag cctccagtac agcgcggctg 2821 aaatcatcat taaagcgagt ggcaacatgg aaatcgctga tttgtgtagt cggtttatgc 2881 agcaacgaga cgtcacggaa aatgccgctc atccgccaca tatcctgatc ttccagataa 2941 ctgccgtcac tccaacgcag caccatcacc gcgaggcggt tttctccggc gcgtaaaaat 3001 gcgctcaggt caaattcaga cggcaaacga ctgtcctggc cgtaaccgac ccagcgcccg 3061 ttgcaccaca gatgaaacgc cgagttaacg ccatcaaaaa taattcgcgt ctggccttcc 3121 tgtagccagc tttcatcaac attaaatgtg agcgagtaac aacccgtcgg attctccgtg 3181 ggaacaaacg gcggattgac cgtaatggga taggttacgt tggtgtagat gggcgcatcg 3241 taaccgtgca tctgccagtt tgaggggacg acgacagtat cggcctcagg aagatcgcac 3301 tccagccagc tttccggcac cgcttctggt accggaaacc aggcaaagcg ccattcgcca 3361 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag 3421 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag 3481 tcacgacgtt gtaaaacgac gggatctatc attggatcct tatcgtcatc gtcgtacaga 3541 tcccgaccca tttgctgtcc accagtcatg ctagccatac catgatgatg atgatgatga 3601 gaacccccca tggaggtgca caccaatgtg gtgaatggtc aaatggcgtt tattgtatcg 3661 agctaggcac ttaaatacaa ttatctctgc aatgcggaat tcagtggttc gtccaatcca 3721 tgtcagacct gtctgttgcc ttcctaataa ggcacgatcg taccacctta cttccaccaa 3781 tcggcatgca cggtgctttt tctctccttg taaggcatgt tgctaactca tcgttaccat 3841 gttgcaagac tacaagtgta ttgcataaga ctacatttcc ccctccctat gcaaaagcga 3901 aactactata tcctgagggg actcctaacc gcgtacaacc gaagccccgc tgggcgccta 3961 aacacaccct agtcccctca gatacgcgta tatctggccc gtacatcgcg aagcagcgca 4021 aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg ccttgttgta 4081 gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg gagcagatac 4141 tggcttaact atgcggcagc agagcagatt gtactgagag tgcaccatac aagctcagga 4201 tctgcgatga taagctgtca aacatgagaa ttggtcgacc actgggcgcc agaaatccgc 4261 gcggtggttt ttgggggtcg ggggtgtttg gcagccacag acgcccggtg ttcgtgtcgc 4321 gccagtacat gcggtccatg cccaggccat ccaaaaacca tgggtctgtc tgctcagtcc 4381 agtcgtggac cagaccccac gcaacgccca aaataataac ccccacgaac cataaaccat 4441 tccccatggg ggaccccgtc cctaacccac ggggccagtg gctatggcag ggcctgccgc 4501 cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg gtggggaaaa 4561 ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga cagagtgcca 4621 gccctgggac cgaaccccgc gtttatgaac aaacgaccca acacccgtgc gttttattct 4681 gtctttttat tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag 4741 ttagcctccc ccatctcccc tattcctttg ccctcggacg agtgctgggg cgtcggtttc 4801 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga 4861 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg 4921 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa 4981 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga 5041 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga 5101 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc 5161 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg 5221 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg 5281 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat 5341 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct 5401 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg 5461 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat 5521 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg 5581 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc 5641 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg 5701 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg 5761 tcgcggtgag ttcaggcttt ttcatatctc attgcccggg atctgcggca cgctgttgac 5821 gctgttaagc gggtcgctgc agggtcgctc ggtgttcgag gccacacgcg tcaccttaat 5881 atgcgaagtg gacctgggac cgcgccgccc cgactgcatc tgcgtgttcg aattcgccaa 5941 tgacaagacg ctgggcgggg tttgtgtcat catagaacta aagacatgca aatatatttc 6001 ttccggggac accgccagca aacgcgagca acgggccacg gggatgaagc agggcatggc 6061 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc 6121 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc 6181 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag 6241 cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac 6301 atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt 6361 gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct 6421 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg 6481 caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg 6541 cgcagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 6601 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 6661 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 6721 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 6781 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 6841 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 6901 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 6961 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 7021 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 7081 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 7141 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7201 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 7261 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 7321 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 7381 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 7441 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 7501 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 7561 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 7621 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac 7681 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 7741 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 7801 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 7861 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 7921 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc 7981 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 8041 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 8101 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 8161 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 8221 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 8281 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 8341 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 8401 ttcgtcttca agaattctca tgtttgacag cttatcatcg cgataagctg atcctcacag 8461 gccgcaccca gcttttcttc cgttgcccca gtagcatctc tgtctggtga ccttgaagag 8521 gaagaggagg ggtcccgaga atccccatcc ctaccgtcca gcaaaaaggg ggacgaggaa 8581 tttgaggcct ggcttgaggc tcaggacgca aatcttgagg atgttcagcg ggagttttcc 8641 gggctgcgag taattggtga tgaggacgag gatggttcgg aggatgggga attttcagac 8701 ctggatctgt ctgacagcga ccatgaaggg gatgagggtg ggggggctgt tggagggggc 8761 aggagtctgc actccctgta ttcactgagc gtcgtctaat aaagatgtct attgatctct 8821 tttagtgtga atcatgtctg acgaggggcc aggtacagga cctggaaatg gcctaggaga 8881 gaagggagac acatctggac cagaaggctc cggcggcagt ggacctcaaa gaagaggggg 8941 tgataaccat ggacgaggac ggggaagagg acgaggacga ggaggcggaa gaccaggagc 9001 cccgggcggc tcaggatcag ggccaagaca tagagatggt gtccggagac cccaaaaacg 9061 tccaagttgc attggctgca aagggaccca cggtggaaca ggagcaggag caggagcggg 9121 aggggcagga gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg 9181 aggggcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggaggggc 9241 aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg 9301 ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg 9361 agcaggagga ggggcaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc 9421 aggagcagga ggaggggcag gagcaggagg ggcaggaggg gcaggagggg caggagcagg 9481 aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag gaggggcagg 9541 agcaggaggg gcaggagcag gaggggcagg agcaggaggg gcaggagggg caggagcagg 9601 aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag gaggggcagg 9661 aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggggcag gaggggcagg 9721 agcaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggagg 9781 ggcaggagca ggaggggcag gagcaggagg tggaggccgg ggtcgaggag gcagtggagg 9841 ccggggtcga ggaggtagtg gaggccgggg tcgaggaggt agtggaggcc gccggggtag 9901 aggacgtgaa agagccaggg ggggaagtcg tgaaagagcc agggggagag gtcgtggacg 9961 tggagaaaag aggcccagga gtcccagtag tcagtcatca tcatccgggt ctccaccgcg 10021 caggccccct ccaggtagaa ggccattttt ccaccctgta ggggaagccg attattttga 10081 ataccaccaa gaaggtggcc cagatggtga gcctgacgtg cccccgggag cgatagagca 10141 gggccccgca gatgacccag gagaaggccc aagcactgga ccccggggtc agggtgatgg 10201 aggcaggcgc aaaaaaggag ggtggtttgg aaagcatcgt ggtcaaggag gttccaaccc 10261 gaaatttgag aacattgcag aaggtttaag agctctcctg gctaggagtc acgtagaaag 10321 gactaccgac gaaggaactt gggtcgccgg tgtgttcgta tatggaggta gtaagacctc 10381 cctttacaac ctaaggcgag gaactgccct tgctattcca caatgtcgtc ttacaccatt 10441 gagtcgtctc ccctttggaa tggcccctgg acccggccca caacctggcc cgctaaggga 10501 gtccattgtc tgttatttca tggtcttttt acaaactcat atatttgctg aggttttgaa 10561 ggatgcgatt aaggaccttg ttatgacaaa gcccgctcct acctgcaata tcagggtgac 10621 tgtgtgcagc tttgacgatg gagtagattt gcctccctgg tttccaccta tggtggaagg 10681 ggctgccgcg gagggtgatg acggagatga cggagatgaa ggaggtgatg gagatgaggg 10741 tgaggaaggg caggagtgat gtaacttgtt aggagacgcc ctcaatcgta ttaaaagccg 10801 tgtattcccc cgcactaaag aataaatccc cagtagacat catgcgtgct gttggtgtat 10861 ttctggccat ctgtcttgtc accattttcg tcctcccaac atggggcaat tgggcatacc 10921 catgttgtca cgtcactcag ctccgcgctc aacaccttct cgcgttggaa aacattagcg 10981 acatttacct ggtgagcaat cagacatgcg acggctttag cctggcctcc ttaaattcac 11041 ctaagaatgg gagcaaccag catgcaggaa aaggacaagc agcgaaaatt cacgccccct 11101 tgggaggtgg cggcatatgc aaaggatagc actcccactc tactactggg tatcatatgc 11161 tgactgtata tgcatgagga tagcatatgc tacccggata cagattagga tagcatatac 11221 tacccagata tagattagga tagcatatgc tacccagata tagattagga tagcctatgc 11281 tacccagata taaattagga tagcatatac tacccagata tagattagga tagcatatgc 11341 tacccagata tagattagga tagcctatgc tacccagata tagattagga tagcatatgc 11401 tacccagata tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc 11461 cagatataaa ttaggatagc atatactacc ctaatctcta ttaggatagc atatgctacc 11521 cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc 11581 cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc 11641 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc 11701 cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccatg 11761 gcaacattag cccaccgtgc tctcagcgac ctcgtgaata tgaggaccaa caaccctgtg 11821 cttggcgctc aggcgcaagt gtgtgtaatt tgtcctccag atcgcagcaa tcgcgcccct 11881 atcttggccc gcccacctac ttatgcaggt attccccggg gtgccattag tggttttgtg 11941 ggcaagtggt ttgaccgcag tggttagcgg ggttacaatc agccaagtta ttacaccctt 12001 attttacagt ccaaaaccgc agggcggcgt gtgggggctg acgcgtgccc ccactccaca 12061 atttcaaaaa aaagagtggc cacttgtctt tgtttatggg ccccattggc gtggagcccc 12121 gtttaatttt cgggggtgtt agagacaacc agtggagtcc gctgctgtcg gcgtccactc 12181 tctttcccct tgttacaaat agagtgtaac aacatggttc acctgtcttg gtccctgcct 12241 gggacacatc ttaataaccc cagtatcata ttgcactagg attatgtgtt gcccatagcc 12301 ataaattcgt gtgagatgga catccagtct ttacggcttg tccccacccc atggatttct 12361 attgttaaag atattcagaa tgtttcattc ctacactagt atttattgcc caaggggttt 12421 gtgagggtta tattggtgtc atagcacaat gccaccactg aaccccccgt ccaaatttta 12481 ttctgggggc gtcacctgaa accttgtttt cgagcacctc acatacacct tactgttcac 12541 aactcagcag ttattctatt agctaaacga aggagaatga agaagcaggc gaagattcag 12601 gagagttcac tgcccgctcc ttgatcttca gccactgccc ttgtgactaa aatggttcac 12661 taccctcgtg gaatcctgac cccatgtaaa taaaaccgtg acagctcatg gggtgggaga 12721 tatcgctgtt ccttaggacc cttttactaa ccctaattcg atagcatatg cttcccgttg 12781 ggtaacatat gctattgaat tagggttagt ctggatagta tatactacta cccgggaagc 12841 atatgctacc cgtttagggt taacaagggg gccttataaa cactattgct aatgccctct 12901 tgagggtccg cttatcggta gctacacagg cccctctgat tgacgttggt gtagcctccc 12961 gtagtcttcc tgggcccctg ggaggtacat gtcccccagc attggtgtaa gagcttcagc 13021 caagagttac acataaaggc aatgttgtgt tgcagtccac agactgcaaa gtctgctcca 13081 ggatgaaagc cactcagtgt tggcaaatgt gcacatccat ttataaggat gtcaactaca 13141 gtcagagaac ccctttgtgt ttggtccccc cccgtgtcac atgtggaaca gggcccagtt 13201 ggcaagttgt accaaccaac tgaagggatt acatgcactg ccccgaatac aaaacaaaag 13261 cgctcctcgt accagcgaag aaggggcaga gatgccgtag tcaggtttag ttcgtccggc 13321 ggcggggc //
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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