出品公司: | Invitrogen |
---|---|
载体名称: | pCMV-RFP-C-HA |
质粒类型: | 哺乳动物表达载体;荧光报告载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 4723 bp |
5' 测序引物及序列: | CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’ |
3' 测序引物及序列: | BGH rev 5''TAGAAGGCACAGTCGAGG3'' |
载体标签: | RFP、HA (C ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | DH5α等 |
宿主细胞(系): | 哺乳动物细胞系 |
备注: | -- |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
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pCMV-RFP-C-HA
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported bp ds-DNA circular SYN 12-AUG-2016 KEYWORDS pCMV-RFP-C-HA REFERENCE 1 (bases 1 to 4723) AUTHORS Biofeng Lab. TITLE Direct Submission FEATURES Location/Qualifiers source 1..4723 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 67..370 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 371..574 /note="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" promoter 625..643 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 650..652 /note="ATG" misc_feature 659..696 /note="MCS" gene 707..1399 /note="RFP" CDS 1400..1426 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /translation="YPYDVPDYA" polyA_signal 1456..1581 /note="bGH poly(A)" /note="bovine growth hormone polyadenylation signal" rep_origin complement(1771..2077) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2121..2225 /gene="bla" /note="bla promoter" promoter 2425..2584 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 2435..2570 /note="SV40 ori" /note="SV40 origin of replication" CDS 2619..3413 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3649..3692 /note="HSV TK poly(A)" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3998..4641 /direction=RIGHT /note="pUC" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 tagttattaa tcccgggagt aatcaattac ggggtcatta gttcatagcc catatatgga 61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 541 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc 601 ttactggctt atcgaaattc tagataatac gactcactat agggcgaata tggccacggg 661 cgcgccctgc aggctagcct cgagggatcc gatatcgcgg ccgcttcttt ccaaacaagt 721 tttgcctcat gatgtgaaaa tgagatacca tatggatgga tgtgttaacg gtcacagttt 781 cactattgag ggagaaggtg ctggtaaacc ttatgaaggc aaaaagatct tggaactgcg 841 tgtaacaaag ggaggaccac ttccattcgc ctttgacatt ttgtccagcg ttttcactta 901 tggtaacagg tgtttctgtg agtatccaga ggatatgccc gactacttta agcaatcatt 961 gcccgagggt cactcttggg agagaacttt aatgtttgag gatggaggat gcggtactgc 1021 ttcagcccat atatcgttgg acaaaaactg tttcgtgcat aagtctacgt ttcatggtgt 1081 gaattttcct gctaatggtc cggtcatgca gaaaaaggca atgaattggg agcctagctc 1141 tgaactgatc accgcatgtg atggtattct caaaggcgac gttaccatgt ttttgttgct 1201 ggaaggtggt cacagattaa agtgccaatt cacaacatcc tacaaggctc ataaagcagt 1261 gaagatgcca ccaaatcaca ttatcgagca tgtcctagtc aaaaaggaag ttgctgacgg 1321 ttttcaaatc caggaacacg ctgttgcaaa acacttcaca gtagatgtta aggaaactcg 1381 cggccgcgaa ttcagatctt acccctacga cgtgcccgac tacgccgtta acttatgata 1441 aggccggcct gatcaaaaat cagcctcgac tgtgccttct agttgccagc catctgttgt 1501 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 1561 ataaaatgag gaaattgcat ctgtacatct agcattcagt tgtggtttgt ccaaactcat 1621 caatttatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt 1681 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 1741 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 1801 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 1861 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 1921 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 1981 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 2041 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg 2101 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 2161 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 2221 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc 2281 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 2341 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 2401 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 2461 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 2521 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 2581 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc 2641 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga 2701 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt 2761 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat 2821 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg 2881 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg 2941 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc 3001 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga 3061 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag 3121 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc 3181 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg 3241 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata 3301 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg 3361 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac 3421 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc 3481 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat 3541 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac 3601 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca 3661 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg 3721 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt 3781 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc 3841 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca 3901 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 3961 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 4021 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 4081 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 4141 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 4201 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 4261 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 4321 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 4381 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 4441 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 4501 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 4561 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 4621 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 4681 gttatcccct gattctgtgg ataaccgtat taccgccatg cat //
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