Citrobacter freundii 弗罗因德氏枸橼酸杆菌8090-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

Citrobacter freundii弗罗因德氏枸橼酸杆菌

(ATCC 8090)

Designation: [ATCC 13316, NCTC 9750]

Deposited Name: Citrobacter freundii (Braak) Werkman and Gillen

Product Description: Type strain. Reference strain. Quality control strain for Sensititre products.

Produces restriction endonuclease CfrAI.

Medium

ATCC® Medium 3: Nutrient agar or nutrient broth

Growth Conditions

Temperature: 37°C

Atmosphere: Aerobic

Propagation Procedure

1. Open vial according to enclosed instructions.

2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or

1.0 mL pipette. Rehydrate the entire pellet.

3. Aseptically transfer this aliquot back into the broth tube. Mix well.

4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.

5. Incubate the tubes and plate at 37°C for 24 to 48 hours.