pRedCas9大肠杆菌RED重组CRISPR/Cas9系统质粒71541-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pRedCas9 plasmid 71541

-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pRedCas9大肠杆菌CRISPR/Cas9介导的可诱导型Red重组系统基因组编辑质粒，组成型表达Cas9，诱导型表达Red重组系统，诱导型表达质粒curing系统，可用于CRISPR介导的大肠杆菌基因组编辑。

Map图谱：

Description:

Constitutiveexpression of Cas9, inducible expression of the Red recombineering system, andinducible expression of the plasmid curing system for CRISPR mediated genomeediting of E. coli.

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• Vector backbone

pSC101ts

• Vector type

Bacterial Expression

• Bacterial Resistance(s)

Spectinomycin

• Growth Temperature

30°C

• Growth Strain(s)

DH5alpha

GENE/INSERT1

• Gene/Insert name

Cas9

• Species

S. pyogenes

GENE/INSERT2

• Gene/Insert name

lambda red genes

CLONINGINFORMATION FOR GENE/INSERT 2

• 5′ sequencing primerM13pUC-Rev

GENE/INSERT3

• Gene/Insert name

plasmid curing system

• Alt name

gRNA targeting the bla gene

• Promoter  pBAD

• 5′ sequencing primerpBAD-F