Klebsiella pneumoniae肺炎克雷伯菌BAA1705-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

Klebsiella pneumoniae肺炎克雷伯菌

Designation: ART 2008133 [D05,

1338]

Product Description: KPC reference strain. This strain is a Klebsiella pneumoniae carbapenemase (KPC)

producer. Modified Hodge Test (MHT) positive control designation. blaKPC positive by PCR.

Medium

ATCC® Medium 18: Trypticase Soy Agar/Broth

Growth Conditions

Temperature: 37°C

Atmosphere: Aerobic

Propagation Procedure

1. Open vial according to enclosed instructions.

2. Using a single tube of #18 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or

1.0 mL pipette. Rehydrate the entire pellet.

3. Aseptically transfer this aliquot back into the broth tube. Mix well.

4. Use several drops of the suspension to inoculate a #18 agar slant and/or plate.

5. Incubate the tubes and plate at 37°C for 24 hours.

blaKPC positive by PCR

This item is not ideal for individual antibiotic resistance testing. For a culture with a consistent detailed

antibiotic pattern, we recommend ordering the Klebsiella pneumoniae QC strains listed in the CLSI manual.