DH5α E.coli strain, competent cells,DH5 alpha，DH5a菌株感受态细胞-BioVector NTCC Inc.

E.coli DH5α strain, competent cells

-BioVector NTCC Inc.

ChromosomalGenotype

fhuA2 lac(del)U169 phoA glnV44 Φ80'lacZ(del)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Description

Transforms with high efficiency. Like manycloning strains, DH5 alpha has several features that make it useful forrecombinant DNA methods.

§TheendA1mutation inactivates an intracellularendonuclease that degrades plasmid DNA in many miniprep methods.

§ThehsdR17mutation eliminates the restrictionendonuclease of the EcoKI restriction-modification system, so DNA lacking theEcoKI methylation will not be degraded. DNA prepared from hsdR strains that arewt forhsdMwill be methylated and can be used totransform wtE. coliK-12 strains.

§Δ(lacZ)M15 is thealpha acceptorallele needed for blue-white screeningwith many lacZ based vectors.

§recA eliminates homologous recombination. This makes thestrain somewhat sickly, but reduces deletion formation and plasmidmultimerization

§glnV44 is the systematic name for SupE44, anamber suppressor

References