Vmax Express Competent Cells

Recombinant Protein Expression: Fast Growth and High Yields of Soluble Protein

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Vmax™ Expressis a novel, fast-growing bacterial strain designed and optimized for high-level recombinant protein expression. This rationally engineered, next-generation prokaryotic protein expression system can serve as a replacement for slow growingE. colisystems that are prone to low yields and the expression of proteins as insoluble inclusion bodies. Vmax™ cells are derived from the marine microorganism,Vibrio natriegens. This gram-negative, non-pathogenic bacterium has a doubling time twice that ofE. coliand robust transcription and translation systems that support this rapid growth rate. This means that Vmax™ Express can produce greater amounts of biomass and generate large amounts of protein in a shorter time period.

With a protein expression workflow similar toE. coli, Vmax™ Express is compatible with plasmids and antibiotics commonly used with bacterial expression systems such asE. coliBL21(DE3). The Vmax™ Express workflow, fast growth, and ability to express a wide range of proteins make it ideal for routine protein expression. Additionally, Vmax™ Express can help overcome common recombinant protein expression challenges such as low yields or the expression of insoluble, or inactive proteins. Built with a tightly controlled, IPTG-inducible T7 promoter system, Vmax™ Express cells can be cultured using routine growth medium, as well as commercial auto-induction media, or ourVmax™ Enriched Growth Media(recommended for rapid growth and greatest accumulation of biomass).

In contrast toE. coli, Vmax™ Express cells grow rapidly at both 30°C and 37°C and tolerate the induction of protein expression over a wide range of starting ODs and IPTG concentrations. In addition, Vmax™ Express cells can be incubated for up to 24 hours post induction to maximize the amount of soluble protein produced. This flexible protein expression workflow and rapid doubling time allows you to go from transformed cells or a glycerol stock to a large-scale culture ready for analysis or protein purification in as few as three standard workdays compared to four days withE. coli.

Summary:

• Fastest growth rate of any known bacterial host with a doubling time of ~14 minutes

• Produces up to twice the biomass ofE. coliwith up to 4X the protein

• Enables expression of high levels of soluble recombinant proteins

• Tightly regulated, IPTG-inducible T7 promoter system

• Compatible with commonly usedE. coliexpression vectors, antibiotics, and growth media

• Protein expression workflow similar to that ofE. coli

• Tolerates induction over wide range OD’s (0.3 to 1.0) and IPTG concentrations (0.5 to 1.0 mM)

• Efficient protein expression up to 24-hours post-induction

• Rapid results - Go from cells to large-scale culture ready for protein purification in three days

E. coliand Vmax™ Express Comparison

Protein Expression Strain Performance Attribute	E. ColiStrains	Vmax™ Express
Compatible with standard plasmid origins of replication (e.g. pMB1, ColE1, pUC, p15A)	✔	✔
Able to use common inexpensive growth media	✔	✔
Plasmid selection uses common antibiotic resistance markers (Amp, Tet, Kan, Cm)	✔	✔
Tightly controlled IPTG Inducible T7 transcription system	✔	✔
Doubling time of ~14 minutes	✖	✔
Biomass >14 OD after 24 hours growth	✖	✔
Flexibility to induce expression at range of OD600	✖	✔
Rapid growth at both 30° and 37°C*	✖	✔
Culture ready for protein purification and analysis in 3 days	✖	✔

*Optimum incubation temperature for Vmax™ Express in liquid media is 30°C. However, the Vmax™ Express growth rate at 37°C exceeds that ofE. coli.