PX551
(Plasmid 60957)

-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pX551腺相关病毒CRISPR/Cas9基因编辑系统载体，mecp2启动子

pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

Map图谱：

Sequence序列：

>PX551 sequence 7400 bps

cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctt

tggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcc

tgcggcctctagaaagcttAGCTGAATGGGGTCCGCCTCTTTTCCCTGCCTAAACAGACAGGAACTCCTG

CCAATTGAGGGCGTCACCGCTAAGGCTCCGCCCCAGCCTGGGCTCCACAACCAATGAAGGGTAATCTCGA

CAAAGAGCAAGGGGTGGGGCGCGGGCGCGCAGGTGCAGCAGCACACAGGCTGGTCGGGAGGGCGGGGCGC

GACGTCTGCCGTGCGGGGTCCCGGCATCGGTTGCGCGCaccggtgccaccatgtacccatacgatgttcc

agattacgcttcgccgaagaaaaagcgcaaggtcgaagcgtccgacaagaagtacagcatcggcctggac

atcggcaccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaagg

tgctgggcaacaccgaccggcacagcatcaagaagaacctgatcggagccctgctgttcgacagcggcga

aacagccgaggccacccggctgaagagaaccgccagaagaagatacaccagacggaagaaccggatctgc

tatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagt

ccttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggc

ctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgac

ctgcggctgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacc

tgaaccccgacaacagcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcga

ggaaaaccccatcaacgccagcggcgtggacgccaaggccatcctgtctgccagactgagcaagagcaga

cggctggaaaatctgatcgcccagctgcccggcgagaagaagaatggcctgttcggcaacctgattgccc

tgagcctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaactgcagctgag

caaggacacctacgacgacgacctggacaacctgctggcccagatcggcgaccagtacgccgacctgttt

ctggccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacaccgagatcacca

aggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaagc

tctcgtgcggcagcagctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgcc

ggctacattgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatgg

acggcaccgaggaactgctcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaa

cggcagcatcccccaccagatccacctgggagagctgcacgccattctgcggcggcaggaagatttttac

ccattcctgaaggacaaccgggaaaagatcgagaagatcctgaccttccgcatcccctactacgtgggcc

ctctggccaggggaaacagcagattcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaa

cttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataag

aacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgtataacgagc

tgaccaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggc

catcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaag

aaaatcgagtgcttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacat

accacgatctgctgaaaattatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctgga

agatatcgtgctgaccctgacactgtttgaggacagagagatgatcgaggaacggctgaaaacctatgcc

cacctgttcgacgacaaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagcc

ggaagctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaagtccgacgg

cttcgccaacagaaacttcatgcagctgatccacgacgacagcctgacctttaaagaggacatccagaaa

gcccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggccggcagccccgccatta

agaagggcatcctgcagacagtgaaggtggtggacgagctcgtgaaagtgatgggccggcacaagcccga

gaacatcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagaga

atgaagcggatcgaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaaca

cccagctgcagaacgagaagctgtacctgtactacctgcagaatgggcgggatatgtacgtggaccagga

actggacatcaaccggctgtccgactacgatgtggaccatatcgtgcctcagagctttctgaaggacgac

tccatcgacaacaaggtgctgaccagaagcgacaagaaccggggcaagagcgacaacgtgccctccgaag

aggtcgtgaagaagatgaagaactactggcggcagctgctgaacgccaagctgattacccagagaaagtt

cgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggcttcatcaagagacag

ctggtggaaacccggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaagtacg

acgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccg

gaaggatttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaac

gccgtcgtgggaaccgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactaca

aggtgtacgacgtgcggaagatgatcgccaagagcgagcaggaaatcggcaaggctaccgccaagtactt

cttctacagcaacatcatgaactttttcaagaccgagattaccctggccaacggcgagatccggaagcgg

cctctgatcgagacaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgc

ggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagacaggcggcttcagcaa

agagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactgggaccctaagaag

tacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaagt

ccaagaaactgaagagtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaa

tcccatcgactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaag

tactccctgttcgagctggaaaacggccggaagagaatgctggcctctgccggcgaactgcagaagggaa

acgaactggccctgccctccaaatatgtgaacttcctgtacctggccagccactatgagaagctgaaggg

ctcccccgaggataatgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatc

gagcagatcagcgagttctccaagagagtgatcctggccgacgctaatctggacaaagtgctgtccgcct

acaacaagcaccgggataagcccatcagagagcaggccgagaatatcatccacctgtttaccctgaccaa

tctgggagcccctgccgccttcaagtactttgacaccaccatcgaccggaagaggtacaccagcaccaaa

gaggtgctggacgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctc

agctgggaggcgacagccccaagaagaagagaaaggtggaggccagctaagaattcAATAAAAGATCTTT

ATTTTCATTAGATCTGTGTGTTGGTTTTTTGTGTgcggccgcaggaacccctagtgatggagttggccac

tccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcc

cgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctcctta

cgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcatta

agcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctt

tcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccc

tttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgt

agtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggac

tcttgttccaaactggaacaacactcaaccctatctcgggctattcttttgatttataagggattttgcc

gatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatatta

acgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgac

acccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgt

gaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggc

ctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcactt

ttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcat

gagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgt

gtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaag

taaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagat

ccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcg

gtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttgg

ttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgc

cataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaacc

gcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagcca

taccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactgg

cgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggacca

cttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtggaagcc

gcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggag

tcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaa

ctgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatct

aggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtc

agaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaa

acaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaagg

taactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccactt

caagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggc

gataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaa

cggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtga

gctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcgga

acaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgcc

acctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaa

cgcggcctttttacggttcctggccttttgctggccttttgctcacatgt