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E.coli DH10B T1 Phage-Resistant strain大肠杆菌菌株感受态细胞DH10B T1R-BioVector NTCC Inc.

  • 价  格:¥17930
  • 货  号:E.coli DH10B T1 Phage-Resistant strain,DH10B T1R
  • 产  地:北京
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E.coli DH10B T1 Phage-Resistant strain, competent cells

E.coli DH10B T1R

-BioVector NTCC Inc.


Features of DH10B T1 Phage-Resistant strain

• Permit efficient cloning of methylated DNA

• Are resistant to T1 and T5 bacteriophages

• Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal

• Deliver high-yield plasmid preparations for downstream applications


Phage-resistant representative libraries from a single transformation

The tonA mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable

libraries against contamination. Additionally, mutations in the methylation-dependent restriction system

(mcrA, mcrBC, and mrr) make DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of

both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes.

DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA

libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the

Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or

Bluo-Gal. Finally, theendA1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for

subsequent extraction and purification.


Genotype:

F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15

ΔlacX74 recA1 endA1araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG tonA


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