DH10B T1 phage resistant E.coli, DH10B T1R BioVector NTCC中国质粒载体菌种细胞基因保藏中心

DH10B T1 phage resistant E.coli, DH10B T1R  

Features of DH10B T1 Phage-Resistant strain:

• Permit efficient cloning of methylated DNA

• Are resistant to T1 and T5 bacteriophages

• Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal

• Deliver high-yield plasmid preparations for downstream applications

Phage-resistant representative libraries from a single transformation

The tonA mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable

libraries against contamination. Additionally, mutations in the methylation-dependent restriction system

(mcrA, mcrBC, and mrr) make DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of

both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes.

DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA

libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the

Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or

Bluo-Gal. Finally, theendA1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for

subsequent extraction and purification.

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15

ΔlacX74 recA1 endA1araD139Δ(ara, leu)7697 galU galK λ-rpsL nupG tonA

Recovery

1. Obtain an LB agar plate with the appropriate antibiotic.

2. Using a sterile pipette tip, touch the bacteria growing within the

punctured area of the stab culture. (A sterilized wire loop or sterile

toothpick can be used in place of a sterile pipette tip.)

3. Run this tip lightly over a section of the plate, as shown in the figure, to

create streak #1.

4. Using another sterile pipette tip, pass through streak #1 and spread

the bacteria over a second section of the plate, to create streak #2.

5. Using a third sterile pipette tip, pass through streak #2 and spread the bacteria over the last section of the plate, to

create streak #3.

6. Grow overnight in a 37 o C incubator (unless a different growth temperature is indicated on the plasmid datasheet).

7. In the morning, single colonies should be visible. If the bacterial growth is too dense, re-streak onto a new agar plate

to obtain single colonies.

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