pNL4-3.Luc.R-E-质粒HIV骨架病毒报告载体Lucferase萤火虫酶荧光表达质粒-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pNL4-3.Luc.R-E-质粒HIV骨架病毒报告载体Lucferase萤火虫酶荧光表达质粒

Firefly luciferase gene was inserted into the pNL4-3 nef gene. Two frameshifts (5′ Env

and Vpr aa 26) render this clone Env - and Vpr-.

Competent for a single round of replication. Requires cotransfection with env

expression vector to produce infectious virus.

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References:

He J, Choe S, Walker R, Di Marzio P, Morgan DO, Landau NR. Human

immunodeficiency virus type 1 viral protein R (Vpr) arrests cells in the G2 phase of

the cell cycle by inhibiting p34cdc2 activity. J Virol 69: 6705–6711, 1995.Abstract

Connor RI, Chen BK, Choe S, Landau NR. Vpr is required for efficient replication of

human immunodeficiency virus type-1 in mononuclear phagocytes. Virology 206:

935–944, 1995.Abstract

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心