首页 » E.coli XL2-Blue MRF′ strain, competent cells-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

E.coli XL2-Blue MRF′ strain, competent cells-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

  • 价  格:¥7920
  • 货  号:XL2-Blue MRF′大肠杆菌菌株感受态细胞
  • 产  地:北京
点击询问我要采购
 竭诚为您服务!
BioVector NTCC典型培养物保藏中心
联系人:Dr.Xu, Biovector NTCC Inc.点击这里给我发消息

电话:+86-010-53513060. 工作QQ:1843439339

邮件:Biovector@163.com

手机:18901268599

地址:北京

已注册
 

E.coli XL2-Blue MRF′ strain, competent cells

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心


Genotype andBackground

Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1supE44 thi-1 recA1 gyrA96 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr) Amy Camr].

(Genes listed signify mutant alleles. Genes on the F′episome, however, are wild-type unless indicated otherwise.)


XL2-Blue MRF′ ultracompetent cells* are high-efficiencyderivatives of XL1-Blue MRF' supercompetent cells. Using ultracompetent celltechnology, it has achieved transformation efficiencies greater than 5 × 10 9 cfu/μg of pUC18DNA, making these cells an ideal choice for high-efficiency cloning. The XL-2Blue MRF' (Minus Restriction) strain is deficient in all known restrictionsystems (McrA-, McrCB-, McrF-, Mrr-, HsdR-), preventingcleavage of cloned DNA that carries cytosine and/or adenine methylation, whichis often present in eukaryotic DNA and cDNA. XL2-Blue MRF′ cells arerestriction-minus [Δ(mcrA)183, Δ(mcrCB-hsdSMR-mrr)173], and areendonuclease (endA), and recombination (recA) deficient. The lacIqZΔM15 gene on the F′episome allows blue-white screening for recombinant plasmids.


            Antibiotic Resistance

XL2-Blue MRF' cells are tetracycline and chloramphenicol resistant.


您正在向 biovector.net  发送关于产品 E.coli XL2-Blue MRF′ strain, competent cells-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 的询问

点击“立即发送”后,我们将在1个工作日内与您取得联系。