p425 GPD，p416 GPD，p426 GPD，p413 TEF，p423 TEF酵母表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

Product Information for NTCC 87669

NTCC Catalog No. 87669

Designation: Yeast Shuttle Expression Vectors

Description: A set of 32 yeast expression vectors

(ATCC 87354 – 87385) differing in promoter (GPD,

TEF, ADH, CYC1), selection marker (HIS3, TRP1,

LEU2, URA3) and replicon (CEN6/ARSH4, 2 micron).

All have the CYC1 terminator.

NOTE: NTCC#87375, p425 ADH is no longer available

and is not distributed in this kit.

Depositor: Martin Funk, Medigene GmbH,

Martinsried/Munchen, Germany

Reference:

Gene 156: 119-122, 1995

Well Vector ATCC# Promoter Replicon Marker

a1 p413 GPD 87354 GPD CEN6/ARSH4 HIS3

a2 p423 GPD 87355 GPD 2 micron HIS3

a3 p414 GPD 87356 GPD CEN6/ARSH4 TRP1

a4 p424 GPD 87357 GPD 2 micron TRP1

a5 p415 GPD 87358 GPD CEN6/ARSH4 LEU2

a6 p425 GPD 87359 GPD 2 micron LEU2

a7 p416 GPD 87360 GPD CEN6/ARSH4 URA3

a8 p426 GPD 87361 GPD 2 micron URA3

a9 p413 TEF 87362 TEF CEN6/ARSH4 HIS3

a10 p423 TEF 87363 TEF 2 micron HIS3

a11 p414 TEF 87364 TEF CEN6/ARSH4 TRP1

a12 p424 TEF 87365 TEF 2 micron TRP1

b1 p415 TEF 87366 TEF CEN6/ARSH4 LEU2

b2 p425 TEF 87367 TEF 2 micron LEU2

b3 p416 TEF 87368 TEF CEN6/ARSH4 URA3

b4 p426 TEF 87369 TEF 2 micron URA3

b5 p413 ADH 87370 ADH CEN6/ARSH4 HIS3

b6 p423 ADH 87371 ADH 2 micron HIS3

b7 p414 ADH 87372 ADH CEN6/ARSH4 TRP1

b8 p424 ADH 87373 ADH 2 micron TRP1

b9 p415 ADH 87374 ADH CEN6/ARSH4 LEU2

b10 p416 ADH 87376 ADH CEN6/ARSH4 URA3

b11 p426 ADH 87377 ADH 2 micron URA3

b12 p413 CYC1 87378 CYC1 CEN6/ARSH4 HIS3

c1 p423 CYC1 87379 CYC1 2 micron HIS3

c2 p414 CYC1 87380 CYC1 CEN6/ARSH4 TRP1

c3 p424 CYC1 87381 CYC1 2 micron TRP1

c4 p415 CYC1 87382 CYC1 CEN6/ARSH4 LEU2

c5 p425 CYC1 87383 CYC1 2 micron LEU2

c6 p416 CYC1 87384 CYC1 CEN6/ARSH4 URA3

c7 p426 CYC1 87385 CYC1 2 micron URA3

Distribution host : Escherichia coli HB101

(ATCC#33694)

Shipped: Frozen glycerol stocks of Escherichia coli

containing plasmid arrayed on a microtiter plate.

Growth temperature : 370C

Growth medium : LB+ampicillin (50 ug/ml)

Propagation:

Transfer a loopful to a test tube containing 5 mL

LB+50g/ml of ampicillin broth. A loopful of culture can

also be streaked on an LB + amp agar plate. Incubate

cultures at 370 C. Isolate DNA using standard plasmid

preparation procedures.

Conditions: Store frozen plate at -800 C.

ATCC Catalog No. 87354

Designation: p413 GPD plasmid in E. coli

Description: Low copy number shuttle expression

vector.

Vector size (kb): 5.876

Type of vector: plasmid

Markers: ampR, HIS3

Cloning sites: SalI ClaI EcoRV EcoRI SmaI BamHI

SpeI XbaI

Features (with orientation and position when

available):

Marker: HIS3, ->, 200-1395

Terminator: CYC1, ->, 2077-2337

MCS: SalI…XbaI, ->, 2343-2401

Promoter for expression: GPD, <-, 2401-3081

Marker: ampR, <-, 4328-5258

Replicon: CEN6/ARSH4, ->, 5363-5876

Restriction digests of the vector give the following sizes

(in kb): SacI/XbaI – 5.2, 0.7; EcoRI – 5.9; BamHI – 5.9.

-------ATCC staff

ATCC Catalog No. 87355

Designation: p423 GPD plasmid in E. coli

Description: High copy number shuttle expression

vector.

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 2 -

Vector size (kb): 6.709

Type of vector: plasmid

Markers: ampR, HIS3

Cloning sites: XhoI SalI ClaI EcoRV EcoRI SmaI

BamHI SpeI

Features (with orientation and position when

available):

Marker: HIS3, ->, 200-1395

Terminator: CYC1, ->, 2077-2337

MCS: XhoI…XbaI, ->, 2337-2401

Promoter for expression: GPD, <-, 2401-3081

Marker: ampR, <-, 4233-5163

Replicon: 2 micron, ->, 5262-6602

Restriction digests of the vector give the following sizes

(in kb): SacI/XbaI – 3.2, 2.8, 0.7; EcoRI – 6.7; BamHI –

6.7. -------ATCC staff

ATCC Catalog No. 87356

Designation: p414 GPD plasmid in E. coli

Description: Low copy number shuttle expression

vector.

Vector size (in kb): 5.694

Type of vector: plasmid

Markers: ampR, TRP1

Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI

SpeI

Features (with orientation and position when

available):

Marker: TRP1, ->, 200-1213

Terminator: CYC1, ->, 1895-2155

MCS: XhoI…XbaI, ->, 2155-2219

Promoter for expression: GPD, <-, 2219-2899

Marker: ampR, <-, 4146-5076

Replicon: CEN6/ARSH4, ->, 5181-5694

Restriction digests of the vector give the following sizes

(in kb): SacI/XbaI – 3.2, 1.9, 0.7; EcoRI – 5.8; BamHI –

5.8. ------------ATCC staff

ATCC Catalog No. 87357

Designation: p424 GPD plasmid in E. coli

Description: High copy number shuttle expression

vector.

Vector size (in kb): 6.527

Type of vector: plasmid

Markers: ampR, TRP1

Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI

SpeI

Features (with orientation and position when

available):

Marker: TRP1, ->

Terminator: CYC1, ->

Promoter for expression: GPD, <-

Marker: ampR, <-

Replicon: 2 micron, ->

Restriction digests of the vector give the following sizes

(in kb): SacI/XbaI – 3.2, 1.7, 1.0, 0.68; EcoRI – 6.5;

BamHI – 6.5. -------ATCC staff

ATCC Catalog No. 87358

Designation: p415 GPD plasmid in E. coli

Description: Low copy number shuttle expression

vector.

Vector size(in kb): 6.927

Type of vector: plasmid

Markers: ampR, LEU2

Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI

XbaI

Features (with orientation and position when

available):

Marker: LEU2, ->, 200-2446

Terminator: CYC1, ->, 3128-3388

MCS: XhoI…XbaI, ->, 3388-3452

Promoter for expression: GPD, <-, 3452-4132

Marker: ampR, <-, 5379-6309

Replicon: CEN6/ARSH4, ->, 6141-6927

Restriction digests of the vector give the following sizes

(in kb): SacI/XbaI – 6.2, 0.7; EcoRI – 4.6, 2.3; BamHI –

6.9. -----------ATCC staff

ATCC Catalog No. 87359

Designation: p425 GPD plasmid in E. coli

Description: High copy number shuttle expression

vector.

Vector size (in kb): 7.760

Type of vector: plasmid

Markers: ampR, LEU2

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 3 -

Cloning sites: XhoI SalI HindIII

PstI SmaI BamHI SpeI

Features (with orientation and position when

available):

Marker: LEU2, ->, 200-2446

Terminator: CYC1, ->, 3128-3388

MCS: XhoI…XbaI, ->, 3388-3452

Promoter for expression: GPD, <-, 3452-4132

Marker: ampR, <-, 5284-6214

Replicon: 2 micron, ->, 6313-7653

Restriction digests of the vector give the following sizes

(in kb): SacI/XbaI – 3.9, 3.2, 0.7; EcoRI – 5.5, 2.3;

BamHI – 7.8. --------------ATCC staff

ATCC Catalog No. 87360

Designation: p416 GPD plasmid in E. coli

Description: Low copy number shuttle expression

vector.

Vector size (in kb): 5.804

Type of vector: plasmid

Markers: ampR, URA3

Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI

BamHI SpeI XbaI

Features (with orientation and position when

available):

Marker: URA3, ->

Terminator: CYC1, ->

Promoter for expression: GPD, <-

Marker: ampR, <-

Replicon: CEN6/ARSH4, ->

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 5.13, 0.68; EcoRI – 5.8;

BamHI – 5.8. ------------ATCC staff

ATCC Catalog No. 87361

Designation: p426 GPD plasmid in E. coli

Description: High copy number shuttle expression

vector.

Vector size (in kb): 6.637

Type of vector: plasmid

Markers: ampR, URA3

Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI

BamHI SpeI

Features (with orientation and position when

available):

Marker: URA3, ->, 200-1300

Terminator: CYC1, ->, 2004-2264

MCS: XhoI…XbaI, ->, 2265-2339

Promoter for expression: GPD, <-, 2339-3019

Marker: ampR, <-, 4138-5068

Replicon: 2 micron, ->, 5167-6507

Restriction digests of the vector give the following sizes

(in kb): SacI/XbaI – 3.2, 2.8, 0.7; EcoRI – 6.6; BamHI –

6.6. ---------ATCC staff

ATCC Catalog No. 87362

Designation: p413 TEF plasmid in E. coli

Description: Low copy number shuttle expression

vector.

Vector size (in kb): 5.598

Type of vector: plasmid

Markers: ampR, HIS3

Cloning sites: XhoI SalI ClaI EcoRV EcoRI SmaI

BamHI SpeI XbaI

Features (with orientation and position when

available):

Marker: HIS3, ->, 200-1395

Terminator: CYC1, ->, 2077-2337

MCS: XhoI…XbaI, ->, 2337-2400

Promoter for expression: TEF, <-, 2401-2803

Marker: ampR, <-, 4050-4980

Replicon: CEN6/ARSH4, ->, 5084-5598

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 5.2, 0.4; XhoI – 5.6; EcoRI –

5.6; BamHI – 5.6. -----------------ATCC staff

ATCC Catalog No. 87363

Designation: p423 TEF plasmid in E. coli

Description: High copy number shuttle expression

vector.

Vector size (in kb): 6.431

Type of vector: plasmid

Markers: ampR, HIS3

Cloning sites: XhoI SalI ClaI EcoRV EcoRI SmaI

BamHI SpeI

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 4 -

Features (with orientation and position when

available):

Marker: HIS3, ->, 200-1395

Terminator: CYC1, ->, 2077-2337

MCS: XhoI…XbaI, ->, 2337-2401

Promoter for expression: TEF, <-, 2401-2803

Marker: ampR, <-, 3954-4884

Replicon: 2 micron, ->, 4984-6324

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.2, 2.8, 0.42; BamHI – 6.4;

XhoI – 6.4. -------------ATCC staff

ATCC Catalog No. 87364

Designation: p414 TEF plasmid in E. coli

Description: Low copy number shuttle expression

vector.

Vector size (in kb): 5.416

Type of vector: plasmid

Markers: ampR, TRP1

Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI

SpeI

Features (with orientation and position when

available):

Marker: TRP1, ->, 200-1213

Terminator: CYC1, ->, 1895-2155

MCS: XhoI…XbaI, ->, 2161-2219

Promoter for expression: TEF, <-, 2219-2621

Marker: ampR, <-, 3868-4798

Replicon: CEN6/ARSH4, ->, 4902-5416

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.1, 1.7, 0.42; EcoRI – 5.6;

BamHI – 5.6. -------------ATCC staff

ATCC Catalog No. 87365

Designation: p424 TEF plasmid in E. coli

Description: High copy number shuttle expression

vector.

Vector size (in kb): 6.249

Type of vector: plasmid

Markers: ampR, TRP1

Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI

SpeI

Features (with orientation and position when

available):

Marker: TRP1, ->, 200-1213

Terminator: CYC1, ->, 1895-2155

MCS: XhoI…XbaI, ->, 2155-2219

Promoter for expression: TEF, <-, 2219-2621

Marker: ampR, <-, 3772-4702

Replicon: 2 micron, ->, 4802-6142

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.2, 1.7, 1.0, 0.44; BamHI –

6.2; EcoRI – 6.2. ----------ATCC staff

ATCC Catalog No. 87366

Designation: p415 TEF plasmid in E. coli

Description: Low copy number shuttle expression

vector.

Vector size (in kb): 6.649

Type of vector: plasmid

Markers: ampR, LEU2

Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI

XbaI

Features (with orientation and position when

available):

Marker: LEU2, ->, 200-2446

Terminator: CYC1, ->, 3128-3388

MCS: XhoI…XbaI, ->, 3388-3452

Promoter for expression: TEF, <-, 3452-3854

Marker: ampR, <-, 5101-6031

Replicon: CEN6/ARSH4, ->, 6135-6649

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 6.2, 0.42; XhoI – 6.6; BamHI

– 6.6. -------------ATCC staff

ATCC Catalog No. 87367

Designation: p425 TEF plasmid in E. coli

Description: High copy number shuttle expression

vector.

Vector size (in kb): 5.526

Type of vector: plasmid

Markers: ampR, LEU2

Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 5 -

Features (with orientation and position when

available):

Marker: LEU2, ->, 200-2446

Terminator: CYC1, ->, 3128-3388

MCS: XhoI…SpeI, ->, 3388-3445

Promoter for expression: TEF, <-, 3452-3854

Marker: ampR, <-, 5005-5935

Replicon: 2 micron, ->, 6035-7375

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.8, 3.2, 0.42; BamHI – 7.8;

EcoRI – 5.0, 2.4. --------------ATCC staff

ATCC Catalog No. 87368

Designation: p416 TEF plasmid in E. coli

Description: Low copy number shuttle expression

vector

Vector size(in kb): 5.526

Type of vector: plasmid

Markers: ampR, URA3

Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI

BamHI SpeI XbaI

Features (with orientation and position when

available):

Marker: URA3, ->, 200-1323

Terminator: CYC1, ->, 2005-2265

MCS: XhoI…XbaI, ->, 2265-2328

Promoter for expression: TEF, <-, 2329-2731

Marker: ampR, <-, 3978-4908

Replicon: CEN6/ARSH4, ->, 5012-5526

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 5.2, 0.42; BamHI – 5.6;

EcoRI – 5.6. ---------------------ATCC staff

ATCC Catalog No. 87369

Designation: p426 TEF plasmid in E. coli

Description: High copy number shuttle expression

vector.

Vector size (in kb): 6.359

Type of vector: plasmid

Markers: ampR, URA3

Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI

BamHI SpeI XbaI

Features (with orientation and position when

available):

Marker: URA3, ->, 200-1300

Terminator: CYC1, ->, 2004-2264

MCS: XhoI…XbaI, ->, 2265-2339

Promoter for expression: TEF, <-, 2339-2741

Marker: ampR, <-, 3859-4789

Replicon: 2 micron, ->, 4889-6229

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.2, 2.7, 0.44; BamHI – 6.5;

XhoI – 6.5. -------------ATCC staff

ATCC Catalog No. 87370

Designation: p413 ADH plasmid in E. coli

Description: Low copy number shuttle expression

vector. The wild type ADH promoter is active when

cells are grown in glucose media but can be repressed

2-10 fold on non-fermentable carbon sources.

Vector size (in kb): 6.696

Type of vector: plasmid

Markers: ampR, HIS3

Cloning sites: XhoI SalI ClaI EcoRV EcoRI SmaI

BamHI SpeI XbaI

Features (with orientation and position when

available):

Marker: HIS3, ->, 200-1395

Terminator: CYC1, ->, 2077-2337

MCS: XhoI…XbaI, ->, 2337-2400

Promoter for expression: ADH, <-, 2401-3901

Marker: ampR, <-, 5148-6078

Replicon: CEN6/ARSH4, ->, 6183-6696

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 5.2, 1.6; BamHI – 6.8; XhoI –

6.8. ------------------ATCC staff

ATCC Catalog No. 87371

Designation: p423 ADH plasmid in E. coli

Description: High copy number shuttle expression

vector. The wild type ADH promoter is active when

cells are grown in glucose media but can be repressed

2-10 fold on non-fermentable carbon sources.

Vector size (in kb): 7.529

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 6 -

Type of vector: plasmid

Markers: ampR, HIS3

Cloning sites: XhoI SalI ClaI EcoRV EcoRI SmaI

BamHI SpeI

Features (with orientation and position when

available):

Marker: HIS3, ->, 200-1395

Terminator: CYC1, ->, 2077-2337

MCS: XhoI…XbaI, ->, 2076-2401

Promoter for expression: ADH, <-, 2401-3901

Marker: ampR, <-, 5053-5983

Replicon: 2 micron, ->, 6082-7422

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.2, 2.8, 1.5; BamHI – 7.6;

EcoRI – 7.6. -------------ATCC staff

ATCC Catalog No. 87372

Designation: p414 ADH plasmid in E. coli

Description: Low copy number shuttle expression

vector. The wild type ADH promoter is active when

cells are grown in glucose media but can be repressed

2-10 fold on non-fermentable carbon sources.

Vector size: 6.514

Type of vector: plasmid

Markers: ampR, TRP1

Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI

SpeI

Features (with orientation and position when

available):

Marker: TRP1, ->, 200-1213

Terminator: CYC1, ->, 1895-2155

MCS: XhoI…XbaI, ->, 2155-2219

Promoter for expression: ADH, <-, 2219-3719

Marker: ampR, <-, 4966-5896

Replicon: CEN6/ARSH4, ->, 6001-6514

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.2, 1.8, 1.5; BamHI – 6.6;

EcoRI – 6.6; XhoI – 6.6. -------------ATCC staff

ATCC Catalog No. 87373

Designation: p424 ADH plasmid in E. coli

Description: High copy number shuttle expression

vector. The wild type ADH promoter is active when

cells are grown in glucose media but can be repressed

2-10 fold on non-fermentable carbon sources.

Vector size(in kb): 7.347

Type of vector: plasmid

Markers: ampR, TRP1

Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI

SpeI

Features (with orientation and position when

available):

Marker: TRP1, ->, 200-1213

Terminator: CYC1, ->, 1895-2155

MCS: XhoI…XbaI, ->, 2155-2219

Promoter for expression: ADH, <-, 2219-3719

Marker: ampR, <-, 4871-5801

Replicon: CEN6/ARSH4, ->, 5900-7240

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.2, 1.7, 1.5, 1.0; BamHI –

7.4; EcoRI – 7.4. ---------ATCC staff

ATCC Catalog No. 87374

Designation: p415 ADH plasmid in E. coli

Description: Low copy number shuttle expression

vector. The wild type ADH promoter is active when

cells are grown in glucose media but can be repressed

2-10 fold on non-fermentable carbon sources.

Vector size (kb): 7.747

Type of vector: plasmid

Markers: ampR, LEU2

Cloning sites: XhoI SalI HindIII PstI SmaI BamHI SpeI

XbaI

Features (with orientation and position when

available):

Marker: LEU2, ->, 200-2446

Terminator: CYC1, ->, 3128-3388

MCS: XhoI…XbaI, ->, 3388-3452

Promoter for expression: ADH, <-, 3452-4952

Marker: ampR, <-, 6199-7129

Replicon: CEN6/ARSH4, ->, 7234-7747

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 6.0, 1.5; EcoRI – 5.4, 2.3;

KpnI – 5.8, 10.7. -----------ATCC staff

ATCC Catalog No. 87376

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 7 -

Designation: p416 ADH

plasmid in E. coli

Description: Low copy number shuttle expression

vector. The wild type ADH promoter is active when

cells are grown in glucose media but can be repressed

2-10 fold on non-fermentable carbon sources.

Vector size (in kb): 6.624

Type of vector: plasmid

Markers: ampR, URA3

Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI

BamHI SpeI XbaI

Features (with orientation and position when

available):

Marker: URA3, ->, 200-1323

Terminator: CYC1, ->, 2005-2265

MCS: XhoI…XbaI, ->, 2265-2328

Promoter for expression: ADH, <-, 2329-3829

Marker: ampR, <-, 5076-6006

Replicon: CEN6/ARSH4, ->, 6111-6624

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 5.1, 1.5; KpnI – 6.6; EcoRI –

6.6. ------------ATCC staff

ATCC Catalog No. 87377

Designation: p426 ADH plasmid in E. coli

Description: High copy number shuttle expression

vector. The wild type ADH promoter is active when

cells are grown in glucose media but can be repressed

2-10 fold on non-fermentable carbon sources

Vector size (in kb): 7.457

Type of vector: plasmid

Markers: ampR, URA3

Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI

BamHI SpeI

Features (with orientation and position when

available):

Marker: URA3, ->, 200-1300

Terminator: CYC1, ->, 2004-2264

MCS: XhoI…XbaI, ->, 2265-2339

Promoter for expression: ADH, <-, 2339-3839

Marker: ampR, <-, 4958-5888

Replicon: CEN6/ARSH4, ->, 5987-7327

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.2, 2.8, 1.5; EcoRI – 7.4;

XbaI – 4.6, 2.7. ------------ATCC staff

ATCC Catalog No. 87378

Designation: p413 CYC1 plasmid in E. coli

Description: Low copy number shuttle expression

vector. Contains a truncated version of the CYC1

promoter that is very weak and is no longer inducible

because of a deletion of most of the UAS2 sequence.

Vector size (in kb): 5.483

Type of vector: plasmid

Markers: ampR, HIS3

Cloning sites: SalI ClaI EcoRV EcoRI SmaI BamHI

SpeI XbaI

Features (with orientation and position when

available):

Marker: HIS3, ->, 200-1395

Terminator: CYC1, ->, 2007-2337

MCS: SalI…XbaI, ->, 2343 - 2400

Promoter for expression: CYC1, <-, 2400-2687

Marker: ampR, <-, 3935-4865

Replicon: CEN6/ARSH4, ->, 4969-5483

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 5.2, 0.3; EcoRI – 5.5; KpnI –

4.5, 0.9. ---------ATCC staff

ATCC Catalog No. 87379

Designation: p423 CYC1 plasmid in E. coli

Description: High copy number shuttle expression

vector. Contains a truncated version of the CYC1

promoter that is very weak and is no longer inducible

because of a deletion of most of the UAS2 sequence.

Vector size (in kb): 6.316

Type of vector: plasmid

Markers: ampR, HIS3

Cloning sites: SalI ClaI EcoRV EcoRI SmaI BamHI

SpeI

Features (with orientation and position when

available):

Marker: HIS3, ->, 200-1395

Terminator: CYC1, ->, 2077-2337

MCS: XhoI…XbaI, ->, 2343-2400

Promoter for expression: CYC1, <-, 2400-2687

Marker: ampR, <-, 3819-4749

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 8 -

Replicon: 2 micron, ->, 4869-

6209

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.2, 2.8, 0.35; XbaI – 3.4,

2.9; HindIII – 4.8, 1.4. --------------ATCC staff

ATCC Catalog No. 87380

Designation: p414 CYC1 plasmid in E. coli

Description: Low copy number shuttle expression

vector. Contains a truncated version of the CYC1

promoter that is very weak and is no longer inducible

because of a deletion of most of the UAS2 sequence.

Vector size (in kb): 5.301

Type of vector: plasmid

Markers: ampR, TRP1

Cloning sites: SalI ClaI EcoRI PstI SmaI BamHI SpeI

Features (with orientation and position when

available):

Marker: TRP1, ->

Terminator: CYC1, ->

MCS: XhoI…XbaI, ->

Promoter for expression: CYC1, <-

Marker: ampR, <-

Replicon: CEN6/ARSH4, ->

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.4, 1.7, 0.3; BamHI – 5.6;

EcoRI – 5.6. ------------ATCC staff

ATCC Catalog No. 87381

Designation: p424 CYC1 plasmid in E. coli

Description: High copy number shuttle expression

vector. Contains a truncated version of the CYC1

promoter that is very weak and is no longer inducible

because of a deletion of most of the UAS2 sequence.

Vector size (in kb): 6.134

Type of vector: plasmid

Markers: ampR, TRP1

Cloning sites: XhoI SalI ClaI HindIII EcoRI SmaI

BamHI SpeI

Features (with orientation and position when

available):

Marker: TRP1, ->

Terminator: CYC1, ->

MCS: XhoI…XbaI, ->

Promoter for expression: CYC1, <-

Marker: ampR, <-

Replicon: 2 micron, ->

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.6, 1.8, 1.1, 0.3; XbaI – 3.5,

1.6, 0.94; BamHI – 6.6; EcoRI – 6.6; SacI – 6.2.

---------------ATCC staff

ATCC Catalog No. 87382

Designation: p415 CYC1 plasmid in E. coli

Description: Low copy number shuttle expression

vector. Contains a truncated version of the CYC1

promoter that is very weak and is no longer inducible

because of a deletion of most of the UAS2 sequence.

Vector size (in kb): 6.534

Type of vector: plasmid

Markers: ampR, LEU2

Cloning sites: SalI HindIII PstI SmaI BamHI SpeI XbaI

Features (with orientation and position when

available):

Marker: LEU2, ->, 200-2446

Terminator: CYC1, ->, 3128-3388

MCS: XhoI…XbaI, ->, 3394-3452

Promoter for expression: CYC1, <-, 3452-3739

Marker: ampR, <-, 4986-5916

Replicon: CEN6/ARSH4, ->, 6000-6514

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 6.2, 0.3; KpnI – 5.0, 1.6;

EcoRI – 4.3, 2.3. ----------ATCC staff

ATCC Catalog No. 87383

Designation: p425 CYC1 plasmid in E. coli

Description: High copy number shuttle expression

vector. Contains a truncated version of the CYC1

promoter that is very weak and is no longer inducible

because of a deletion of most of the UAS2 sequence.

Vector size (in kb): 7.367

Type of vector: plasmid

Markers: ampR, LEU2

Cloning sites: SalI HindIII PstI SmaI BamHI SpeI

Features (with orientation and position when

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 9 -

available):

Marker: LEU2, ->, 200-2446

Terminator: CYC1, ->, 3128-3388

MCS: XhoI…XbaI, ->, 3394-3452

Promoter for expression: CYC1, <-, 3452-3739

Marker: ampR, <-, 4870-5800

Replicon: 2 micron, ->, 5920-7260

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.9, 3.2, 0.35; XbaI – 3.9,

3.5; KpnI – 6.0, 1.4. -----------ATCC staff

ATCC Catalog No. 87384

Designation: p416 CYC1 plasmid in E. coli

Description: Low copy number shuttle expression

vector. Contains a truncated version of the CYC1

promoter that is very weak and is no longer inducible

because of a deletion of most of the UAS2 sequence.

Vector size (in kb): 5.411

Type of vector: plasmid

Markers: ampR, URA3

Cloning sites: SalI ClaI HindIII EcoRI SmaI BamHI

SpeI XbaI

Features (with orientation and position when

available):

Marker: URA3, ->, 200-1323

Terminator: CYC1, ->, 2005-2265

MCS: SalI…XbaI, ->, 2271-2328

Promoter for expression: CYC1, <-, 2329-2616

Marker: ampR, <-, 3863-4793

Replicon: CEN6/ARSH4, ->, 4887-5391

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 5.1, 0.3; KpnI – 5.4; EcoRI –

5.4. ----------ATCC staff

ATCC Catalog No. 87385

Designation: p426 CYC1 plasmid in E. coli

Description: High copy number shuttle expression

vector. Contains a truncated version of the CYC1

promoter that is very weak and is no longer inducible

because of a deletion of most of the UAS2 sequence.

Vector size (in kb): 6.244

Type of vector: plasmid

Markers: ampR, URA3

Cloning sites: SalI ClaI HindIII EcoRI SmaI BamHI

SpeI

Features (with orientation and position when

available):

Marker: URA3, ->, 200-1300

Terminator: CYC1, ->, 2004-2264

MCS: SalI…XbaI, ->, 2271-2339

Promoter for expression: CYC1, <-, 2339-2626

Marker: ampR, <-, 3724-4654

Replicon: 2 micron, ->, 4774-6114

Restriction digests of the vector give the following

sizes (in kb): SacI/XbaI – 3.2, 2.75. 0.35; XbaI – 3.4,

2.9; EcoRI – 6.3.

Biosafety Level: 1

Appropriate safety procedures should always be used with

this material. Laboratory safety is discussed in the current

publication of the Biosafety in Microbiological and Biomedical

Laboratories, from the U.S. Department of Health and

Human Services Centers for Disease Control and Prevention

and National Institutes for Health.

ATCC Warranty

The viability of ATCC products is warranted for 30 days from

the date of shipment. If you feel there is a problem with this

product, contact Technical Services by phone at 800-638-

6597 or 703-365-2700 or by e-mail at tech@atcc.org. Or you

may contact your local distributor.

Disclaimers

This product is intended for laboratory research purposes

only. It is not intended for use in humans.

While ATCC uses reasonable efforts to include accurate and

up-to-date information on this product sheet, ATCC makes

no warranties or representations as to its accuracy. Citations

from scientific literature and patents are provided for

informational purposes only. ATCC does not warrant that

such information has been confirmed to be accurate.

This product is sent with the condition that you are

responsible for its safe storage, handling, and use. ATCC is

not liable for any damages or injuries arising from receipt

and/or use of this product. While reasonable effort is made to

insure authenticity and reliability of strains on deposit, ATCC

is not liable for damages arising from the misidentification or

misrepresentation of cultures.

Please see the enclosed Material Transfer Agreement (MTA)

for further details regarding the use of this product. The

MTA is also available on our Web site at www.atcc.org.

© ATCC 2009. All rights reserved.

ATCC® is a registered trademark of the American Type Culture

Collection.

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 10 -

[Updated 5/25/10 LMH]

Maps of Constructs included in ATCC 87669

ATCC 87354

ATCC 87356

ATCC 87355

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 11 -

ATCC 87357

ATCC 87358

ATCC 87360

ATCC 87362

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 12 -

ATCC 87359

ATCC 87361

ATCC 87363

ATCC 87364

ATCC 87366

ATCC 87368

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 13 -

ATCC 87365

ATCC 87367

ATCC 87369

ATCC 87371

ATCC 87373

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 14 -

ATCC 87370

ATCC 87372

ATCC 87374

ATCC 87376

ATCC 87378

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 15 -

ATCC 87380

ATCC 87377

ATCC 87379

ATCC 87381

Product Information Sheet for ATCC 87669

American Type Culture Collection 800-638-6597 or 703-365-2700

P.O. Box 1549 Fax: 703-365-2750

Manassas, VA 20108 USA E-mail: tech@atcc.org

www.atcc.org Or contact your local distributor.

- 16 -

ATCC 87382

ATCC 87384

ATCC 87383

ATCC 87385