MSP1830 plasmid BioVector NTCC中国质粒载体菌种细胞基因保藏中心 70708

• Vector backbone
  pCAG-CFP
  (Search Vector Database)
• Backbone manufacturer
  Cepko lab (Addgene plasmid# 11179)
• Vector type
  Mammalian Expression, CRISPR

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

GENE/INSERT

• Gene/Insert name
  mammalian codon-optimized KKH variant Staphylococcus aureus Cas9(E782K/N968K/R1015H)-NLS-3xFlag
• Species
  S. aureus
• Mutation
  E782K, N968K and R1015H in SaCas9

• PromoterCAG

• Tags / Fusion Proteins

  • NLS (C terminal on insert)

  • 3x FLAG (C terminal on insert)

  
  

CLONING INFORMATION

• Cloning methodUnknown

• 5′ sequencing primeroBK280 (5'-CCTACAGCTCCTGGGCAACG-3')