首页 » E.coli Origami (DE3)大肠杆菌表达宿主菌-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

E.coli Origami (DE3)大肠杆菌表达宿主菌-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

  • 价  格:¥5932
  • 货  号:E.coli Origami (DE3)大肠杆菌表达宿主菌
  • 产  地:北京
点击询问我要采购
 竭诚为您服务!
BioVector NTCC典型培养物保藏中心
联系人:Dr.Xu, Biovector NTCC Inc.

电话:400-800-2947 工作QQ:1843439339 (微信同号)

邮件:Biovector@163.com

手机:18901268599

地址:北京

已注册
 

E.coli Origami (DE3)大肠杆菌表达宿主菌

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net


Origami host strains areK-12 derivatives that have mutations in both the thioredoxin reductase (trxB)and glutathione reductase (gor) genes, which greatly enhance disulfidebond formation in theE. colicytoplasm.Studies have shown that expression in Origami(DE3) yielded 10-fold more activeprotein than in another host even though overall expression levels weresimilar. The original Origami strains are compatible with ampicillin-resistantplasmids and are ideal for use with pET-32 vectors, since the thioredoxinfusion tag further enhances the formation of disulfide bonds in the cytoplasm.ThetrxBandgormutations areselectable on kanamycin and tetracycline, respectively; therefore, thesestrains cannot be used with plasmids that can only be selected with kanamycinor tetracycline. To reduce the possibility of disulfide bond formation betweenmolecules, strains containing mutations intrxBandgorare recommendedonly for the expression of proteins that require disulfide bond formation forproper folding.

DE3
indicates thatthe host is a lysogen of λDE3, and therefore carries a chromosomal copy of theT7 RNA polymerase gene under control of thelacUV5promoter. Suchstrains are suitable for production of protein from target genes cloned in pETvectors by induction with IPTG.


Genotype:Δ(ara-leu)7697ΔlacX74 ΔphoA PvuIIphoR araD139ahpC galE galK rpsLF′[lac+lacIqpro] (DE3)gor522::Tn10trxB(KanR,StrR, TetR)


您正在向 biovector.net  发送关于产品 E.coli Origami (DE3)大肠杆菌表达宿主菌-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心 的询问

点击“立即发送”后,我们将在1个工作日内与您取得联系。