BL21(DE3) E. coli大肠杆菌表达宿主菌-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BL21(DE3) E. coli大肠杆菌表达宿主菌

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net

Description

BL21(DE3)E. coliare ideal foruse with bacteriophage T7 promoter-based expression systems (e.g.,pRSET, pCR¤T7, and pET). BL21(DE3) carry the lambda DE3 lysogen. Recombinantproteins that are non-toxic toE. coliare generallyexpressed at higher levels in BL21(DE3) cells than in BL21(DE3)pLysS orBL21(DE3)pLysE. However, the basal expression levels of heterologous genes aresignificantly higher in BL21(DE3) than in BL21(DE3)pLysS or BL21(DE3)pLysE.

Genotype

F – ompT hsdSB (rB– mB–) gal dcm(DE3)