OVCAR-3人上皮性卵巢癌细胞株-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

Organism

Homo  sapiens, human

Tissue

ovary

Cell Type

epithelial

Product Format

frozen

Morphology

epithelial

Culture  Properties

adherent

Biosafety  Level

1

Disease

adenocarcinoma

Age

60 years

Gender

female

Ethnicity

Caucasian

Applications

This cell line is a suitable transfection host.

 NIH:OVCAR-3 is an appropriate model system in which to study drug resistance  in ovarian cancer, and the presence of hormone receptors should be useful for  the evaluation of hormonal therapy.

Karyotype

The cell line is aneuploid human female, with  chromosome counts in the sub to near-triploid range. Several normal  chromosomes (N11, N13, N14, N15, N16, N17, and N22) are clearly  under-represented. Many of these missing chromosomes are represented in the large  number of cytogenetically altered chromosomes identified as marker  chromosomes. In addition to the marker chromosomes, there are a large number  of other structurally abnormal and unassignable chromosomes that are not  recognized as markers. Random loss and gain of chromosomes from cell to cell  are noted in the exact chromosome counts and in the analysis of the  karyotypes.

Images

Derivation

The NIH:OVCAR-3 line was established in 1982 by T.C.  Hamilton, et al. from the malignant ascites of a patient with progressive  adenocarcinoma of the ovary.

Clinical Data

Caucasian

female

60 years

Receptor  Expression

Androgen receptor, positive; estrogen receptor,  positive; progesterone receptor, positive

Tumorigenic

Yes

Effects

Yes, Forms colonies in soft agar

Yes, in nude mice inoculated subcutaneously with 10(7)  cells

(Tumors developed within 21 days at 100% frequency  (5/5).)

Comments

Forms colonies in soft agar and has an abnormal  karyotype.

Resistant to clinically relevant concentrations of  adriamycin, melphalan and cisplatin.

Both cultured cells and xenografts exhibit androgen and  estrogen receptors.

Xenograft models have been used to show that treatment  with 17 beta estradiol can induce progesterone receptors in this human  ovarian carcinoma.

Subculturing

Volumes used in this protocol are for 75 sq cm flasks;  proportionally reduce or increase amount of dissociation medium for culture  vessels of other sizes. Corning® T-75 flasks (catalog #430641) are  recommended for subculturing this product.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with  Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v)  Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains  trypsin inhibitor.

3.Add 2.0 to 3.0 mL of Trypsin-EDTA  solution to flask and observe cells under an inverted microscope until cell  layer is dispersed (usually within 5 to 15 minutes).
 Note: To avoid clumping do not agitate the cells by hitting or shaking the  flask while waiting for the cells to detach. Cells that are difficult to  detach may be placed at 37°C to facilitate dispersal.

4.Add 2.0 to 3.0 mL of complete growth  medium and aspirate cells by gently pipetting

5.Resuspend the cell pellet in fresh growth  medium. Add appropriate aliquots of the cell suspension to new culture  vessels.

6.Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of  1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO,  5%

Storage temperature: liquid nitrogen vapor  temperature

Culture  Conditions

Temperature: 37°C

 Atmosphere: air, 95%; carbon dioxide (CO2), 5%

STR Profile

Amelogenin: X

CSF1PO: 11,12

D13S317: 12

D16S539: 12

D5S818: 11,12

D7S820: 10

THO1: 9,9.3

TPOX: 8

vWA: 17

Isoenzymes

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 1

PGM3, 1

References

Hamilton TC, et al. Characterization of a human ovarian  carcinoma cell line (NIH:OVCAR-3) with androgen and estrogen receptors.  Cancer Res. 43: 5379-5389, 1983. PubMed: 6604576

Rogan AM, et al. Reversal of adriamycin resistance by  verapamil in human ovarian cancer. Science 224: 994-996, 1984. PubMed:6372095