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Stbl4大肠杆菌菌株,化学感受态细胞-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

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Stbl4 E.coli strain, Chemically Competent Cells

Stbl4大肠杆菌菌株,化学感受态细胞

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net

产品套装编号:NTCC161186

储存条件:-80保存保存时间:12


Stbl4™ cells are E. coli cells which can be transformed by

electroporation (1,2). These cells can only be transformed by

electroporation and are not transformed by "heat shock". These cells are

suitable for the generation of cDNA libraries using plasmid-derived

vectors. The lacZΔM15 marker provides α-complementation of the

β-galactosidase gene from pUC or similar vectors and therefore can be

used for blue/white screening of colonies on agar plates containing Xgal

or Bluo-gal and IPTG. The mcrA mutation and the mcrBC-hsdRMSmrr

deletion allow cloning of genomic sequences which are methylated.

Stbl4™ is a derivative of Stbl2™ (3) and is suitable for the cloning of

unstable inserts such as retroviral sequences or direct repeats (3,4,5).

Unlike Stbl2™, Stbl4™ is lon+. For optimal performance, expression in

S.O.C. Medium as well as incubation on antibiotic plates should be

done at 30°C*. Stbl4™ cells are capable of being transformed efficiently

with large plasmids and can also serve as a host for M13mp cloning

vectors (see Note 1).

*To maximize stabilization of direct repeats and retroviral sequences,

incubate Stbl4™cells and perform expression studies at 30°C.


Genotype

mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 gyrA96 gal- thi-1 supE44 λ- relA1

Δ(lac-proAB)/F′ proAB+ lacIqZΔM15 Tn10 (TetR)


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