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pMito-iRFP713人细胞色素c氧化酶亚基VIII荧光表达质粒 -BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

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  • 货  号:pMito-iRFP713人细胞色素c氧化酶亚基VIII荧光表达质粒
  • 产  地:北京
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质粒基本信息

质粒名称:pMito-iRFP713


目录编号:45465
存储实验室:Vladislav Verkhusha

载体骨架基本信息
载体名称:pN1
载体出品公司:Clontech
载体抗性:卡那霉素
空载体大小:4038 bp
完整质粒大小:4989 bp
载体修饰:人细胞色素c氧化酶亚基VIII(human cytochrome C oxidase subunit VIII)
载体类型:哺乳细胞表达
筛选标记:新霉素(G418)
高拷贝/低拷贝:高拷贝
生长条件:37 ℃

插入基因信息
插入基因名称:iRFP713
物种来源:光合细菌 Rhodopseudomonas palustris
插入大小:951 bp
基因突变:T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2
GenBank编号JN247409
启动子CMV
标签/融合蛋白:人细胞色素c氧化酶亚基VIII(N端)
克隆方法:限制性内切酶
5' 克隆位点:AgeI(未破坏)
3' 克隆位点:NotI (未破坏)
5' 测序引物CMV-F
3' 测序引物SV40pA-R

文献引用方法


参考文献部分:
Near-infrared fluorescent proteins for multicolor in vivo imaging. Shcherbakova DM, Verkhusha VV. Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521. 10.1038/nmeth.2521 PubMed 23770755

质粒图谱信息

pMito-iRFP713质粒图谱

质粒简介


质粒序列

LOCUS       pMito-iRFP713	4989 bp  circular	DNA	SYN
DEFINITION  pMito-iRFP713
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biovector.net
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|www.biovector.net|
COMMENT     VNTNAME|pMito-iRFP713|
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="pMito-iRFP713"
                     /mol_type="other DNA"
     promoter        10..562
                     /label="CMV_immearly_promoter"
     misc_feature    65..352
                     /label="CAG_enhancer"
     CDS             complement(447..1034)
                     /label="ORF frame 2"
     misc_feature    519..539
                     /label="CMV_fwd_primer"
     promoter        520..589
                     /label="CMV_promoter"
     CDS             597..1655
                     /label="ORF frame 3"
     misc_feature    1868..1887
                     /label="EBV_rev_primer"
     rep_origin      complement(2037..2343)
                     /label="f1_origin"
     promoter        2422..2450
                     /label="AmpR_promoter"
     misc_feature    complement(2516..2536)
                     /label="pBABE_3_primer"
     misc_feature    complement(2522..2737)
                     /label="SV40_enhancer"
     promoter        2534..2802
                     /label="SV40_promoter"
     rep_origin      2701..2778
                     /label="SV40_origin"
     misc_feature    2763..2782
                     /label="SV40pro_F_primer"
     CDS             2885..3679
                     /label="ORF frame 2"
     gene            2888..3676
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(3194..3730)
                     /label="ORF frame 3"
     terminator      3854..4123
                     /label="TK_PA_terminator"
     rep_origin      4271..4890
                     /label="pBR322_origin"
ORIGIN
    1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
   61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
  121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
  181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
  241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
  301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
  361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
  421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
  481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
  541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgt
  601 ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc
  661 cgcgcgccaa gatccattcg ttgggggatc caccggtcgc caccatggcg gaaggatccg
  721 tcgccaggca gcctgacctc ttgacctgcg acgatgagcc gatccatatc cccggtgcca
  781 tccaaccgca tggactgctg ctcgccctcg ccgccgacat gacgatcgtt gccggcagcg
  841 acaaccttcc cgaactcacc ggactggcga tcggcgccct gatcggccgc tctgcggccg
  901 atgtcttcga ctcggagacg cacaaccgtc tgacgatcgc cttggccgag cccggggcgg
  961 ccgtcggagc accgatcact gtcggcttca cgatgcgaaa ggacgcaggc ttcatcggct
 1021 cctggcatcg ccatgatcag ctcatcttcc tcgagctcga gcctccccag cgggacgtcg
 1081 ccgagccgca ggcgttcttc cgccgcacca acagcgccat ccgccgcctg caggccgccg
 1141 aaaccttgga aagcgcctgc gccgccgcgg cgcaagaggt gcggaagatt accggcttcg
 1201 atcgggtgat gatctatcgc ttcgcctccg acttcagcgg cgaagtgatc gcagaggatc
 1261 ggtgcgccga ggtcgagtca aaactaggcc tgcactatcc tgcctcaacc gtgccggcgc
 1321 aggcccgtcg gctctatacc atcaacccgg tacggatcat tcccgatatc aattatcggc
 1381 cggtgccggt caccccagac ctcaatccgg tcaccgggcg gccgattgat cttagcttcg
 1441 ccatcctgcg cagcgtctcg cccgtccatc tggaattcat gcgcaacata ggcatgcacg
 1501 gcacgatgtc gatctcgatt ttgcgcggcg agcgactgtg gggattgatc gtttgccatc
 1561 accgaacgcc gtactacgtc gatctcgatg gccgccaagc ctgcgagcta gtcgcccagg
 1621 ttctggcctg gcagatcggc gtgatggaag agtgagcggc cgcgactcta gatcataatc
 1681 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
 1741 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
 1801 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
 1861 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
 1921 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
 1981 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
 2041 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
 2101 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
 2161 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
 2221 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
 2281 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
 2341 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
 2401 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
 2461 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
 2521 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
 2581 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
 2641 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
 2701 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
 2761 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
 2821 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
 2881 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
 2941 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
 3001 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
 3061 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
 3121 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
 3181 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
 3241 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
 3301 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
 3361 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
 3421 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
 3481 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
 3541 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
 3601 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
 3661 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
 3721 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
 3781 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
 3841 ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
 3901 cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat
 3961 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
 4021 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
 4081 ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat
 4141 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
 4201 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
 4261 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
 4321 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
 4381 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
 4441 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
 4501 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
 4561 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
 4621 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
 4681 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
 4741 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
 4801 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
 4861 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
 4921 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
 4981 gccatgcat
//


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