pGL4.19[luc2CP/Neo] Vector荧光报告载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

pGL4.19[luc2CP/Neo] Vector荧光报告载体

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net

pGL4.19荧光报告载体，带有luc2CP萤火虫酶荧光报告基因，Neo-G418筛选标记，Amp抗性

The pGL4.19[luc2CP/Neo] Vector(a–d) encodes the luciferase reporter gene luc2CP (Photinus pyralis) and isdesigned for high expression and reduced anomalous transcription. This vector also contains a mammalian selectablemarker for neomycin resistance in which the number of transcription factor-binding sites has been reduced and mammaliancodon usage optimized. This vector is engineered with fewer consensus regulatory sequences for reduced background and adecreased risk of anomolous transcription and has a synthetic reporter gene, which is codon-optimized for mammalianexpression.The pGL4.19[luc2CP/Neo] Vector is a basic vector with no promoter. However, the vector contains a multiple cloning regionthat allows cloning of a promoter of choice. The luc2CP reporter gene contains two protein destabilization sequences: hCL1and hPEST. The protein encoded by luc2CP responds more quickly and with greater magnitude to changes in transcriptionalactivity than the luc2 gene, its more stable counterpart.

Map图谱