pSpCas9n(BB)-2A-Puro BioVector NTCC中国质粒载体菌种细胞基因保藏中心

• Vector backbone
  PX462
  (Search Vector Database)

• Total vector size (bp)9200

• Vector type
  Mammalian Expression, CRISPR
• Selectable markers
  Puromycin

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  Stbl3
• Copy number
  Unknown

GENE/INSERT

• Gene/Insert name
  hSpCas9n-2A-Puro
• Alt name
  SpCas9n-2A-Puro
• Alt name
  Cas9n
• Alt name
  PX462
• Species
  Synthetic
• Insert Size (bp)
  6000
• Mutation
  Cas9 D10A nickase mutant

• PromoterCbh

• Tags / Fusion Proteins

  • 3XFLAG (N terminal on insert)

  • 2A-Puro (C terminal on insert)

  
  

CLONING INFORMATION

• Cloning methodRestriction Enzyme

• 5′ cloning siteAgeI (not destroyed)

• 3′ cloning siteEcoRI (not destroyed)

• 5′ sequencing primerTTTATGGCGAGGCGGCGG