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pSpCas9n(BB)-2A-Puro BioVector NTCC中国质粒载体菌种细胞基因保藏中心

  • 价  格:¥7932
  • 货  号:pSpCas9n(BB)-2A-Puro plasmid 48141
  • 产  地:北京
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pSpCas9n(BB)-2A-Puro

BioVector NTCC中国质粒载体菌种细胞基因保藏中心                            [Supplier来源]  http://www.biovector.net


描述:

pSpCas9n(BB)-2A-Puro (PX462)基因编辑CRISPR-Cas9载体,U6启动子+gRNA,puro嘌呤霉素细胞筛选标记,Amp抗性。

Note that this is the plasmid used in the original publication. The Puro gene in this plasmid has a point mutation that renders it less effective in some cell lines. The new version of the plasmid has corrected that point mutation, thus improving Puro selection.


  • Vector backbone
    PX462
    (Search Vector Database)
  • Total vector size (bp)9200

  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

GENE/INSERT

  • Gene/Insert name
    hSpCas9n-2A-Puro
  • Alt name
    SpCas9n-2A-Puro
  • Alt name
    Cas9n
  • Alt name
    PX462
  • Species
    Synthetic
  • Insert Size (bp)
    6000
  • Mutation
    Cas9 D10A nickase mutant
  • PromoterCbh

  • Tags / Fusion Proteins

    • 3XFLAG (N terminal on insert)

    • 2A-Puro (C terminal on insert)


CLONING INFORMATION

  • Cloning methodRestriction Enzyme

  • 5′ cloning siteAgeI (not destroyed)

  • 3′ cloning siteEcoRI (not destroyed)

  • 5′ sequencing primerTTTATGGCGAGGCGGCGG


Map图谱


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