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pWPXLd慢病毒绿色荧光表达载体-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

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  • 货  号:pWPXLd慢病毒绿色荧光表达载体
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pWPXLd慢病毒绿色荧光表达载体

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net


EF1-alpha启动子,Amp抗性。

pWPXLd is essentially pWPXL without the LoxP site in the 3' LTR.

pWPT or pWPXL can be used for constitutive transgene expression.
pWPXL contains the EF-1alpha promoter + intron that gives you high expression, as RNA loves to be spliced (it goes more efficiently out of the nucleus). pWPT contains only the EF-1alpha promoter

Unique restriction sites at key positions will allow you to change promoter and transgene. PmeI is a popular site for cloning. You can also use PacI and SwaI.

Please note that ClaI in these vectors is blocked by Dam methylation.
The plasmids need to be grown in a Dam- bacteria strain, if you wish to use ClaI for cloning.


Map图谱




  • Vector backbone
    pWPXLd
    (Search Vector Database)
  • Backbone size (bp)10455

  • Vector type
    Mammalian Expression, Lentiviral

GROWTH IN BACTERIA

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
  • Copy number
    High Copy

GENE/INSERT

  • Gene/Insert name
    None
  • Tag / Fusion Protein

    • EGFP


CLONING INFORMATION

  • Cloning methodRestriction Enzyme

  • 5′ cloning siteMCS - see map (not destroyed)

  • 3′ cloning siteMCS - see map (not destroyed)

  • 5′ sequencing primerSee map



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