pDR244枯草芽孢杆菌基因敲除载体 BioVector NTCC中国质粒载体菌种细胞基因保藏中心
- 价 格:¥29532
- 货 号:pDR244枯草芽孢杆菌基因敲除载体
- 产 地:北京
- BioVector NTCC典型培养物保藏中心
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pDR244枯草芽孢杆菌基因敲除载体 BioVector NTCC中国质粒载体菌种细胞基因保藏中心 Loop-out Plasmid for Generating Markerless Deletions
in the Bacillus subtilis BKE Knockout Collection. cre/lox-Mediated Loop-out Protocol for pDR244
(1) Transform pDR224 into one of the Bacillus subtilis BKE collection or any other strain harboring a
loxP- anked antibiotic resistance cassette with selection for spectinomycin resistance at 30°C
(2) Transfer several transformant colonies to plain LB plates and incubate overnight at 42°C
(3) Screen resulting colonies for plasmid curing (spectinomycin sensitivity) and the antibiotic
resitance cassette (erythromycin sensitivity).
(4) Streak for fresh single colonies and then confirm loss of the cassette by PCR. BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net [Supplier来源] http://www.biovector.net
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