pGK1.0-Fut8 BioVector NTCC中国质粒载体菌种细胞基因保藏中心

pGK1.0-Fut8 BioVector NTCC中国质粒载体菌种细胞基因保藏中心 pGK1.0-Fut8 Cat No.:BioVector591612 Backbone vector: pGK1.0 ■基因名称：Fut8 Gene ID：100751648 英文全称：fucosyltransferase 8 (alpha (1,6) fucosyltransferase) 物种来源：CHO ■Function Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAcresidue, next to the peptide chains in N-glycans. ■Pathway Protein modification; protein glycosylation. ■Sequence similarities Belongs to the glycosyltransferase 23 family. Contains 1 SH3 domain. ■Cellular localization Golgi apparatus > Golgi stack membrane. Membrane-bound form in transcisternae of Golgi. ■Database links Entrez Gene: 100751648 CHO Standard heat-shock transformation of chemically competent bacteria 1. Take competent cells out of -80°C and thaw on ice. 2. Take agar plates (containing the appropriate antibiotic) out of 4°C to warm up to room temperature or place in 37°C incubator. 3. Mix 5μl of DNA into 100μL competent cells in a microcentrifuge or falcon tube. GENTLY mix by flicking the bottom of the tube with your finger a few times. 4. Place the competent cell/DNA mixture on ice for 20-30min. 5. Heat shock each transformation tube by placing the bottom 2/3 of the tube into a 42°C water bath for 42 seconds. Put the tubes back on ice for 2 min. 6. Add 250-500μl SOC media (without antibiotic) and grow in 37°C shaking incubator for 45min. 7. Plate all of the transformation onto a 10cm LB agar plate containing the appropriate antibiotic. 8. Incubate plates at 37°C overnight. [Supplier来源] http://www.biovector.net