pWPI二代双顺反子慢病毒荧光表达载体Cre-Lox同源重组系统质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pWPI二代双顺反子慢病毒荧光表达载体Cre-Lox同源重组系统质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心                      

pWPI二代双顺反子慢病毒荧光表达载体Cre-Lox同源重组系统质粒，同时表达目的基因和EGFP绿色荧光标记基因，助力转化后示踪，骨架载体pWPXL,EF1-alpha启动子。

(Empty Backbone) 2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.   This bicistronic vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells. The EGFP marker cDNA has been inserted downstream of EMCV IRES.A popular cloning strategy is to clone into the PmeI site upstream of the EMCV IRES. You can also clone into the PacI or SwaI site.If you want to put another fluorescent protein after the IRES, note that you need to make sure that the open reading frame of the second gene is precisely fused to 11th ATG of IRES (i.e., 11th ATG of IRES is a start of your cDNA).BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心www.biovector.net      [Supplier来源]  http://www.biovector.net