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pET2 Shuttle promoter-probe vector BioVector NTCC质粒载体菌种细胞基因保藏中心

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pET2 Shuttle promoter-probe vector  BioVector NTCC质粒载体菌种细胞基因保藏中心                    




LOCUS       AJ885178                7509 bp    DNA     circular SYN 15-APR-2005DEFINITION  Shuttle promoter-probe vector pET2.ACCESSION   AJ885178VERSION     AJ885178.1KEYWORDS    aminoglycoside phosphotransferase; aph gene; cat gene;            chloramphenicol acetyltransferase; rep gene; replicase.SOURCE      Shuttle promoter-probe vector pET2  ORGANISM  Shuttle promoter-probe vector pET2            other sequences; artificial sequences; vectors.REFERENCE   1  AUTHORS   Eikmanns,B.J., Kleinertz,E., Liebl,W. and Sahm,H.  TITLE     A family of Corynebacterium glutamicum/Escherichia coli shuttle            vectors for cloning, controlled gene expression, and promoter            probing  JOURNAL   Gene 102 (1), 93-98 (1991)   PUBMED   1864513REFERENCE   2  AUTHORS   Vasicova,P., Abrhamova,Z., Nesvera,J., Patek,M., Sahm,H. and            Eikmanns,B.  TITLE     Integrative and autonomously replicating vectors for analysis of            promoters in Corynebacterium glutamicum  JOURNAL   Biotech. Tech. 12, 743-746 (1998)REFERENCE   3  (bases 1 to 7509)  AUTHORS   Wendisch,V.F.  TITLE     Direct Submission  JOURNAL   Submitted (23-FEB-2005) Wendisch V.F., Institute of Biotechnology            1, Research Center Juelich, Juelich, D-52425, GERMANYFEATURES             Location/Qualifiers     source          1..7509                     /organism="Shuttle promoter-probe vector pET2"                     /mol_type="other DNA"                     /db_xref="taxon:315474"                     /lab_host="Escherichia coli/Corynebacterium glutamicum"                     /focus     source          47..1496                     /organism="Cloning vector pKK232-8"                     /mol_type="other DNA"                     /db_xref="taxon:74582"                     /lab_host="Escherichia coli"     source          1497..7020                     /organism="Shuttle-expression vector pEKEx2"                     /mol_type="other DNA"                     /db_xref="taxon:272747"                     /lab_host="Corynebacterium glutamicum"     source          7021..7266                     /organism="Corynebacterium glutamicum"                     /mol_type="other DNA"                     /db_xref="taxon:1718"     source          7267..7509                     /organism="Escherichia coli"                     /mol_type="other DNA"                     /db_xref="taxon:562"     misc_feature    1..46                     /note="multiple cloning site"     gene            108..767                     /gene="cat"     CDS             108..767                     /gene="cat"                     /EC_number="2.3.1.28"                     /function="chloramphenicol resistance"                     /experiment="experimental evidence, no additional details                     recorded"                     /citation=[1]                     /codon_start=1                     /transl_table=11                     /product="chloramphenicol acetyltransferase"                     /protein_id="CAI59794.1"                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG                     A"     gene            2733..3548                     /gene="aph"     CDS             2733..3548                     /gene="aph"                     /function="kanamycine resistance"                     /experiment="experimental evidence, no additional details                     recorded"                     /codon_start=1                     /transl_table=11                     /product="aminoglycoside phosphotransferase"                     /protein_id="CAI59795.1"                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"     gene            5164..6447                     /gene="rep"     CDS             5164..6447                     /gene="rep"                     /function="plasmid replication in Corynebacterium                     glutamicum"                     /experiment="experimental evidence, no additional details                     recorded"                     /codon_start=1                     /transl_table=11                     /product="replicase"                     /protein_id="CAI59796.1"                     /translation="MNTSKEPQVNEGSKVTRARAWRRQNVMYKITNSKALAGCHRWRR                     DEAVAVSWSSNGASQFEGLQNSHSRWGSPLAELEVMGERRIELAIATKNHLAAGGALM                     MFVGTVRHNRSQSFAQVEAGIKTAYSSMVKTSQWKKERARYGVEHTYSDYEVTDSWAN                     GWHLHRNMLLFLDRPLSDDELKAFEDSMFSRWSAGVVKAGMDAPLREHGVKLDQVSTW                     GGDAAKMATYLAKGMSQELTGSATKTASKGSYTPFQMLDMLADQSDAGEDMDAVLVAR                     WREYEVGSKNLRSSWSRGAKRALGIDYIDADVRREMEEELYKLAGLEAPERVESTRVA                     VALVKPDDWKLIQSDFAVRQYVLDCVDKAKDVAAAQRVANEVLASLGVDSTPCMIVMD                     DVDLDAVLPTHGDAIKRDLNAAVFAGNEQTILRTH"ORIGIN              1 ctgcaggtcg actctagagg atccccgggt accgagctcg gtacccagct tgagtaggac       61 aaatccgccg agcttcgacg agattttcag gagctaagga agctaaaatg gagaaaaaaa      121 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat      181 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt      241 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc      301 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat      361 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc      421 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg      481 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg      541 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca      601 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga      661 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc      721 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca      781 gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga      841 atggcagaaa ttcgtcgagg cggcacctcg ctaacggatt caccactcca agaattggag      901 ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca      961 tcgcgtccgc catctccagc agccgcacgc ggcgcatctc ggctgttttg gcggatgaga     1021 gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa     1081 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa     1141 acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc     1201 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt     1261 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc     1321 aacggcccgg agggtggcgg gcaggacgcc ataaactgcc aggcatcaaa ttaagcagaa     1381 ggccatcctg acggatggcc tttttgcgtt tctacaaact cttcctgtcg tcatatctac     1441 aagccatccc cccacagata cggtaaacta gcctcgtttt tgcatcagga aagcagaatt     1501 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa     1561 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac     1621 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca     1681 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc     1741 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc     1801 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc     1861 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag     1921 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc     1981 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt     2041 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct     2101 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg     2161 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct     2221 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat     2281 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg     2341 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa     2401 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt     2461 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc     2521 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt     2581 atcaaaaagg atcttcacct agatcctttt gggggggggg ggaaagccac gttgtgtctc     2641 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt     2701 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt     2761 gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc     2821 gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc     2881 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg     2941 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta     3001 ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca ttccaggtat     3061 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc     3121 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg     3181 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc     3241 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg ccattctcac     3301 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga     3361 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg     3421 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa     3481 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt     3541 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac     3601 gggacggcgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca     3661 tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc     3721 cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc     3781 gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgcccccc     3841 cccccctagc ttgtctacgt ctgatgcttt gaatcggacg gacttgccga tcttgtatgc     3901 ggtgattttt ccctcgtttg cccacttttt aatggtggcc ggggtgagag ctacgcgggc     3961 ggcgacctgc tgcgctgtga tccaatattc ggggtcgttc actggttccc ctttctgatt     4021 tctggcatag aagaaccccc gtgaactgtg tggttccggg ggttgctgat ttttgcgaga     4081 cttctcgcgc aattccctag cttaggtgaa aacaccatga aacactaggg aaacacccat     4141 gaaacaccca ttagggcagt agggcggctt cttcgtctag ggcttgcatt tgggcggtga     4201 tctggtcttt agcgtgtgaa agtgtgtcgt aggtggcgtg ctcaatgcac tcgaacgtca     4261 cgtcatttac cgggtcacgg tgggcaaaga gaactagtgg gttagacatt gttttcctcg     4321 ttgtcggtgg tggtgagctt ttctagccgc tcggtaaacg cggcgatcat gaactcttgg     4381 aggttttcac cgttctgcat gcctgcgcgc ttcatgtcct cacgtagtgc caaaggaacg     4441 cgtgcggtga ccacgacggg cttagccttt gcctgcgctt ctagtgcttc gatggtggct     4501 tgtgcctgcg cttgctgcgc ctgtagtgcc tgttgagctt cttgtagttg ctgttctagc     4561 tgtgccttgg ttgccatgct ttaagactct agtagctttc ctgcgatatg tcatgcgcat     4621 gcgtagcaaa cattgtcctg caactcattc attatgtgca gtgctcctgt tactagtcgt     4681 acatactcat atttacctag tctgcatgca gtgcatgcac atgcagtcat gtcgtgctaa     4741 tgtgtaaaac atgtacatgc agattgctgg gggtgcaggg ggcggagcca ccctgtccat     4801 gcggggtgtg gggcttgccc cgccggtaca gacagtgagc accggggcac ctagtcgcgg     4861 ataccccccc taggtatcgg acacgtaacc ctcccatgtc gatgcaaatc tttaacattg     4921 agtacgggta agctggcacg catagccaag ctaggcggcc accaaacacc actaaaaatt     4981 aatagttcct agacaagaca aacccccgtg cgagctacca actcatatgc acgggggcca     5041 cataacccga aggggtttca attgacaacc atagcactag ctaagacaac gggcacaaca     5101 cccgcacaaa ctcgcactgc gcaaccccgc acaacatcgg gtctaggtaa cactgaaata     5161 gaagtgaaca cctctaagga accgcaggtc aatgagggtt ctaaggtcac tcgcgctagg     5221 gcgtggcgta ggcaaaacgt catgtacaag atcaccaata gtaaggctct ggcggggtgc     5281 cataggtggc gcagggacga agctgttgcg gtgtcctggt cgtctaacgg tgcttcgcag     5341 tttgagggtc tgcaaaactc tcactctcgc tgggggtcac ctctggctga attggaagtc     5401 atgggcgaac gccgcattga gctggctatt gctactaaga atcacttggc ggcgggtggc     5461 gcgctcatga tgtttgtggg cactgttcga cacaaccgct cacagtcatt tgcgcaggtt     5521 gaagcgggta ttaagactgc gtactcttcg atggtgaaaa catctcagtg gaagaaagaa     5581 cgtgcacggt acggggtgga gcacacctat agtgactatg aggtcacaga ctcttgggcg     5641 aacggttggc acttgcaccg caacatgctg ttgttcttgg atcgtccact gtctgacgat     5701 gaactcaagg cgtttgagga ttccatgttt tcccgctggt ctgctggtgt ggttaaggcc     5761 ggtatggacg cgccactgcg tgagcacggg gtcaaacttg atcaggtgtc tacctggggt     5821 ggagacgctg cgaaaatggc aacctacctc gctaagggca tgtctcagga actgactggc     5881 tccgctacta aaaccgcgtc taaggggtcg tacacgccgt ttcagatgtt ggatatgttg     5941 gccgatcaaa gcgacgccgg cgaggatatg gacgctgttt tggtggctcg gtggcgtgag     6001 tatgaggttg gttctaaaaa cctgcgttcg tcctggtcac gtggggctaa gcgtgctttg     6061 ggcattgatt acatagacgc tgatgtacgt cgtgaaatgg aagaagaact gtacaagctc     6121 gccggtctgg aagcaccgga acgggtcgaa tcaacccgcg ttgctgttgc tttggtgaag     6181 cccgatgatt ggaaactgat tcagtctgat ttcgcggtta ggcagtacgt tctagattgc     6241 gtggataagg ctaaggacgt ggccgctgcg caacgtgtcg ctaatgaggt gctggcaagt     6301 ctgggtgtgg attccacccc gtgcatgatc gttatggatg atgtggactt ggacgcggtt     6361 ctgcctactc atggggacgc tattaagcgt gatctgaatg cggcggtgtt cgcgggtaat     6421 gagcagacta ttcttcgcac ccactaaaag cggcataaac cccgttcgat attttgtgcg     6481 atgaatttat ggtcaatgtc gcgggggcaa actatgatgg gtcttgttgt tgacaatggc     6541 tgatttcatc aggaatggaa ctgtcatgct gttatgtgcc tggctcctaa tcaaagctgg     6601 ggacaatggg ttgccccgtt gatctgatct agttcggatt ggcggggctt cactgtatct     6661 gggggtggca tcgtgaatag attgcacacc gtagtgggca gtgtgcacac catagtggcc     6721 atgagcacca ccacccccag ggacgccgac ggcgcgaagc tctgcgcctg gtgcggctcg     6781 gagatcaagc aatccggcgt cggccggagc cgggactact gccgccgctc ctgccgccag     6841 cgggcgtacg aggcccggcg ccagcgcgag gcgatcgtgt ccgccgtggc gtcggcagtc     6901 gctcgccgag atacgtcacg tgacgaaatg cagcagcctt ccattccgtc acgtgacgaa     6961 actcgggccg caggtcagag cacggttccg cccgctccgg ccctgccgga cccccggctg     7021 catcgcagct gatgtggctg gccgagataa ctctcagccg atttctacca ctgaggtggg     7081 agaccgcatc gtcaaggcgc tgcaaagcta aatttcaacg ccgaccccct tgccttttat     7141 ggcgaggggt ttacttttcg gtatgggttg aattggttgg tgtaaatact tgctggattg     7201 atatcgggga cgtcgtattg gcggtcaatc cagtgggacc acatgctgaa ctccctatca     7261 ctgcatgagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg     7321 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg     7381 ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca     7441 taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt     7501 ctacaaact            [Supplier来源]  http://www.biovector.net

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