pRK5-HA-Ubiquitin-WT BioVector-017608 BioVector NTCC质粒载体菌种细胞基因保藏中心

pRK5-HA-Ubiquitin-WT  BioVector-017608 BioVector NTCC质粒载体菌种细胞基因保藏中心                      

Mammalian expression of HA tagged ubiquitin. The wild-type ubiquitin sequence in this plasmid is: MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG.    Vector backbone pRK5-HA (Search Vector Database)Backbone size w/o insert (bp) 4800Vector type Mammalian ExpressionGROWTH IN BACTERIABacterial Resistance(s) AmpicillinGrowth Temperature 37°CGrowth Strain(s) DH5alphaCopy number High CopyGENE/INSERTGene/Insert name Ubiquitin CAlt name UbSpecies H. sapiens (human)Insert Size (bp) 220Entrez Gene UBC (a.k.a. HMG20)Tag / Fusion ProteinHA (N terminal on backbone)CLONING INFORMATIONCloning method Restriction Enzyme5′ cloning site SalI (not destroyed)3′ cloning site NotI (not destroyed)5′ sequencing primer SP6      [Supplier来源]  http://www.biovector.net