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BS1365大肠杆菌宿主菌 BioVector NTCC质粒载体菌种细胞基因保藏中心

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BS1365大肠杆菌宿主菌

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心


Genotype:

BS1365 (BS591 F’ kan (BS591: recA1 endA1 gyrA96 thi-1 D lacU169 supE44hsdR17 [lambda imm434 nin5 X1-cre])


Description:    

This is an E. coli strainexpressing cre recombinase, originally created by Brian Sauer,

described in Sauer, B.and N. Henderson, The cyclization of linear DNA in Escherichia coli bysite-specific recombination. Gene, 1988. 70: p. 331-341.


Recovery

1.Obtainan LB agar plate with the appropriate antibiotic.

2.Usinga sterile pipette tip, touch the bacteria growing within the punctured area ofthe stab culture. (A sterilized wire loop or sterile toothpick can be used inplace of a sterile pipette tip.)

3.Runthis tip lightly over a section of the plate, as shown in the figure, to createstreak #1.

4.Usinganother sterile pipette tip, pass through streak #1 and spread the bacteriaover a second section of the plate, to create streak #2.

5.Usinga third sterile pipette tip, pass through streak #2 and spread the bacteriaover the last section of the plate, to create streak #3.

6.Growovernight in a 37 o C incubator (unless a different growthtemperature is indicated on the plasmid datasheet).

7.Inthe morning, single colonies should be visible. If the bacterial growth is toodense, re-streak onto a new agar plate to obtain single colonies.


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